Diagnóstico e genotipagem do helicobacter pylori em amostras de biópsia gástrica, saliva e placa dental
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da USC |
Texto Completo: | http://localhost:8080/tede/handle/tede/164 |
Resumo: | Purpose: The Helicobacter pylori, a gram negative bacterium to colonize human gastric mucosa and has been shown to be strongly associated with chronic gastritis, gastric and duodenal ulcerations and a risk factor for gastric carcinoma. Several studies suggest progressive gastric mucosa damage induced by the patogen. Extensive search for Helicobacter pylori virulence factors lead to the characterizations of a vacuolating cytotoxin A (vacA) and an associated cytotoxin A (cagA). The oral cavity has been implicated as a potential H. pylori reservoir, and may therefore be involved in the reinfection of the stomach, which sometimes follows treatment of H. pylori infection. The objectives of this study were (1) to determine the presence of H. pylori in the oral cavity and (2) to investigate the relationship between oral H. pylori and subsequent gastritis. Methods: Gastric biopsies, saliva and dental plaques were obtained from dyspeptic adults. DNA was extracted and evaluated for the H. pylori and cagA presence, vacA alleles, using polymerase chain reaction and Southern blotting methods. Results: Persons with gastritis had a high frequency of Helicobacter pylori positives in the stomach and there was a statistically significant correlation between gastric biopsies and oral cavity. Conclusions: We shown a high prevalence of the H. pylori and presence with cagA gene associated of vacA s1 alelles in gastric biopsies. Our results suggest a relation between gastric infection and the bacterium in the oral cavity, with a variation of cytotoxin genotype between saliva and dental plaque suggesting a reservoir of the species and that more than one H. pylori strains may exist in the saliva, dental plaque and stomach of the same patient |
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Payão, Spencer Luiz MarquesCPF:07139122865http://lattes.cnpq.br/8583444557745535CPF:07139122865http://lattes.cnpq.br/9152072043915486Rasmussen, Lucas Trevizani2015-09-14T13:13:08Z2010-06-082009-08-28RASMUSSEN, Lucas Trevizani. Diagnosis and genotyping of Helicobacter pylori in gastric biopsy specimens, saliva and dental plaque. 2009. 78 f. Dissertação (Mestrado em Ciências da Saúde e Biológicas) - IASCJ - Universidade Sagrado Coração, Bauru, 2009.http://localhost:8080/tede/handle/tede/164Purpose: The Helicobacter pylori, a gram negative bacterium to colonize human gastric mucosa and has been shown to be strongly associated with chronic gastritis, gastric and duodenal ulcerations and a risk factor for gastric carcinoma. Several studies suggest progressive gastric mucosa damage induced by the patogen. Extensive search for Helicobacter pylori virulence factors lead to the characterizations of a vacuolating cytotoxin A (vacA) and an associated cytotoxin A (cagA). The oral cavity has been implicated as a potential H. pylori reservoir, and may therefore be involved in the reinfection of the stomach, which sometimes follows treatment of H. pylori infection. The objectives of this study were (1) to determine the presence of H. pylori in the oral cavity and (2) to investigate the relationship between oral H. pylori and subsequent gastritis. Methods: Gastric biopsies, saliva and dental plaques were obtained from dyspeptic adults. DNA was extracted and evaluated for the H. pylori and cagA presence, vacA alleles, using polymerase chain reaction and Southern blotting methods. Results: Persons with gastritis had a high frequency of Helicobacter pylori positives in the stomach and there was a statistically significant correlation between gastric biopsies and oral cavity. Conclusions: We shown a high prevalence of the H. pylori and presence with cagA gene associated of vacA s1 alelles in gastric biopsies. Our results suggest a relation between gastric infection and the bacterium in the oral cavity, with a variation of cytotoxin genotype between saliva and dental plaque suggesting a reservoir of the species and that more than one H. pylori strains may exist in the saliva, dental plaque and stomach of the same patientA bactéria Helicobacter pylori coloniza aproximadamente 50% da população mundial e está associada à gênese de gastrites, úlceras e câncer gástrico. Diferentes cepas de importância clínica vem sendo descritas por pesquisadores, que sugerem diversos marcadores de virulência entre os quais podemos destacar o gene cagA e o gene vacA que em vários estudos estão relacionados a gastrites e úlceras pépticas. Há controversas sobre a permanência do H. pylori na cavidade oral, e a literatura não é clara em afirmar se a cavidade oral é um reservatório da bactéria. O presente trabalho tem como objetivos diagnosticar o Helicobacter pylori e caracterizar a presença do gene cagA e os genótipos do gene vacA através da técnica de PCR e Southern Blotting, a partir de amostras de biópsia gástrica, saliva e placa dental. Foram analisadas amostras de biópsia gástrica, saliva e placa dental coletadas de pacientes adultos dispépticos. Houve uma diferença estatisticamente significante na detecção do H. pylori por Southern blotting e as demais técnicas, também verificamos um aumento significativo de positividade para o H. pylori associado ao gene cagA e alelos s1/m1 do gene vacA em amostras de biópsia gástrica, já as amostras de saliva e placa dental apresentaram uma menor prevalência da bactéria sem associação com os marcadores de virulência, entretanto a presença do H. pylori, em amostras de saliva e placa dental reforçam a premissa de que cavidade oral seja um reservatório da bactéria, e uma possível via de transmissão e reinfecção gástrica. Além disso, evidenciamos uma colonização mista em um único hospedeiro assim como a ausência de relação efetiva entre a colonização da saliva, placa dental e mucosa gástricaMade available in DSpace on 2015-09-14T13:13:08Z (GMT). No. of bitstreams: 1 dissertacao_lucas_trevizani_rasmussen.pdf: 1428671 bytes, checksum: c1f8fc337c685ee705434ba2d9e19e3a (MD5) Previous issue date: 2009-08-28Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESPapplication/pdfporIASCJ - Universidade Sagrado CoraçãoBiologia OralUSCBRCiências da Saúde e BiológicasHELICOBACTER PYLORISALIVAPLACA DENTALCAGAVACASOUTHERN BLOTTINGHELICOBACTER PYLORISALIVADENTAL BOARDCAGAVACASOUTHERN BLOTTINGCIENCIAS DA SAUDE::ODONTOLOGIADiagnóstico e genotipagem do helicobacter pylori em amostras de biópsia gástrica, saliva e placa dentalDiagnosis and genotyping of Helicobacter pylori in gastric biopsy specimens, saliva and dental plaqueinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8043334104093724546600600600600-753428887677182963-2070498469879244349-6491868300948288337info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da USCinstname:Universidade do Sagrado Coração (USC)instacron:USCORIGINALdissertacao_lucas_trevizani_rasmussen.pdfapplication/pdf1428671http://localhost:8080/tede/bitstream/tede/164/1/dissertacao_lucas_trevizani_rasmussen.pdfc1f8fc337c685ee705434ba2d9e19e3aMD51tede/1642015-10-16 17:20:12.944oai:localhost:tede/164Biblioteca Digital de Teses e Dissertaçõeshttps://tede2.usc.br:8443/http://tede2.usc.br:8080/oai/requestbiblicorjesu@unisagrado.edu.br||normalizacao@usc.bropendoar:2015-10-16T20:20:12Biblioteca Digital de Teses e Dissertações da USC - Universidade do Sagrado Coração (USC)false |
dc.title.por.fl_str_mv |
Diagnóstico e genotipagem do helicobacter pylori em amostras de biópsia gástrica, saliva e placa dental |
dc.title.alternative.eng.fl_str_mv |
Diagnosis and genotyping of Helicobacter pylori in gastric biopsy specimens, saliva and dental plaque |
title |
Diagnóstico e genotipagem do helicobacter pylori em amostras de biópsia gástrica, saliva e placa dental |
spellingShingle |
Diagnóstico e genotipagem do helicobacter pylori em amostras de biópsia gástrica, saliva e placa dental Rasmussen, Lucas Trevizani HELICOBACTER PYLORI SALIVA PLACA DENTAL CAGA VACA SOUTHERN BLOTTING HELICOBACTER PYLORI SALIVA DENTAL BOARD CAGA VACA SOUTHERN BLOTTING CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Diagnóstico e genotipagem do helicobacter pylori em amostras de biópsia gástrica, saliva e placa dental |
title_full |
Diagnóstico e genotipagem do helicobacter pylori em amostras de biópsia gástrica, saliva e placa dental |
title_fullStr |
Diagnóstico e genotipagem do helicobacter pylori em amostras de biópsia gástrica, saliva e placa dental |
title_full_unstemmed |
Diagnóstico e genotipagem do helicobacter pylori em amostras de biópsia gástrica, saliva e placa dental |
title_sort |
Diagnóstico e genotipagem do helicobacter pylori em amostras de biópsia gástrica, saliva e placa dental |
author |
Rasmussen, Lucas Trevizani |
author_facet |
Rasmussen, Lucas Trevizani |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Payão, Spencer Luiz Marques |
dc.contributor.advisor1ID.fl_str_mv |
CPF:07139122865 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8583444557745535 |
dc.contributor.authorID.fl_str_mv |
CPF:07139122865 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9152072043915486 |
dc.contributor.author.fl_str_mv |
Rasmussen, Lucas Trevizani |
contributor_str_mv |
Payão, Spencer Luiz Marques |
dc.subject.por.fl_str_mv |
HELICOBACTER PYLORI SALIVA PLACA DENTAL CAGA VACA SOUTHERN BLOTTING |
topic |
HELICOBACTER PYLORI SALIVA PLACA DENTAL CAGA VACA SOUTHERN BLOTTING HELICOBACTER PYLORI SALIVA DENTAL BOARD CAGA VACA SOUTHERN BLOTTING CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
HELICOBACTER PYLORI SALIVA DENTAL BOARD CAGA VACA SOUTHERN BLOTTING |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Purpose: The Helicobacter pylori, a gram negative bacterium to colonize human gastric mucosa and has been shown to be strongly associated with chronic gastritis, gastric and duodenal ulcerations and a risk factor for gastric carcinoma. Several studies suggest progressive gastric mucosa damage induced by the patogen. Extensive search for Helicobacter pylori virulence factors lead to the characterizations of a vacuolating cytotoxin A (vacA) and an associated cytotoxin A (cagA). The oral cavity has been implicated as a potential H. pylori reservoir, and may therefore be involved in the reinfection of the stomach, which sometimes follows treatment of H. pylori infection. The objectives of this study were (1) to determine the presence of H. pylori in the oral cavity and (2) to investigate the relationship between oral H. pylori and subsequent gastritis. Methods: Gastric biopsies, saliva and dental plaques were obtained from dyspeptic adults. DNA was extracted and evaluated for the H. pylori and cagA presence, vacA alleles, using polymerase chain reaction and Southern blotting methods. Results: Persons with gastritis had a high frequency of Helicobacter pylori positives in the stomach and there was a statistically significant correlation between gastric biopsies and oral cavity. Conclusions: We shown a high prevalence of the H. pylori and presence with cagA gene associated of vacA s1 alelles in gastric biopsies. Our results suggest a relation between gastric infection and the bacterium in the oral cavity, with a variation of cytotoxin genotype between saliva and dental plaque suggesting a reservoir of the species and that more than one H. pylori strains may exist in the saliva, dental plaque and stomach of the same patient |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-08-28 |
dc.date.available.fl_str_mv |
2010-06-08 |
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2015-09-14T13:13:08Z |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
RASMUSSEN, Lucas Trevizani. Diagnosis and genotyping of Helicobacter pylori in gastric biopsy specimens, saliva and dental plaque. 2009. 78 f. Dissertação (Mestrado em Ciências da Saúde e Biológicas) - IASCJ - Universidade Sagrado Coração, Bauru, 2009. |
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http://localhost:8080/tede/handle/tede/164 |
identifier_str_mv |
RASMUSSEN, Lucas Trevizani. Diagnosis and genotyping of Helicobacter pylori in gastric biopsy specimens, saliva and dental plaque. 2009. 78 f. Dissertação (Mestrado em Ciências da Saúde e Biológicas) - IASCJ - Universidade Sagrado Coração, Bauru, 2009. |
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por |
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8043334104093724546 |
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600 600 600 600 |
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Biologia Oral |
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USC |
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BR |
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Ciências da Saúde e Biológicas |
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