Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/210030 |
Resumo: | Objective: This study aimed to evaluate the cytotoxicity and synergistic effect of epigallocatechin gallate (EGCG) and fosfomycin (FOSFO) on biofilms of oral bacteria associated with endodontic infections. Methodology: This study determined minimum inhibitory and bactericidal concentration (MIC/MBC) and fractionated inhibitory concentration (FIC) of EGCG and FOSFO against Enterococcus faecalis, Actinomyces israelii, Streptococcus mutans, and Fusobacterium nucleatum. Monospecies and multispecies biofilms with those bacteria formed in polystyrene microplates and in radicular dentin blocks of bovine teeth were treated with the compounds and control chlorhexidine (CHX) and evaluated by bacterial counts and microscopy analysis. Toxicity effect of the compounds was determined on fibroblasts culture by methyl tetrazolium assays. Results: The combination of EGCG + FOSFO demonstrated synergism against all bacterial species, with an FIC index ranging from 0.35 to 0.5. At the MIC/FIC concentrations, EGCG, FOSFO, and EGCG+FOSFO were not toxic to fibroblasts. EGCG+FOSFO significantly reduced monospecies biofilms of E. faecalis and A. israelli, whereas S. mutans and F. nucleatum biofilms were eliminated by all compounds. Scanning electron microscopy of multispecies biofilms treated with EGCG, EGCG+FOSFO, and CHX at 100x MIC showed evident biofilm disorganization and substantial reduction of extracellular matrix. Confocal microscopy observed a significant reduction of multispecies biofilms formed in dentin tubules with 84.85%, 78.49%, and 50.6% of dead cells for EGCG+FOSFO, EGCG, and CHX at 100x MIC, respectively. Conclusion: EGCG and fosfomycin showed a synergistic effect against biofilms of oral pathogens related to root canal infections without causing cytotoxicity. |
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Journal of applied oral science (Online) |
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Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infectionsEpigallocatechin gallateFosfomycinDrug synergismBiofilmsCytotoxicity testsObjective: This study aimed to evaluate the cytotoxicity and synergistic effect of epigallocatechin gallate (EGCG) and fosfomycin (FOSFO) on biofilms of oral bacteria associated with endodontic infections. Methodology: This study determined minimum inhibitory and bactericidal concentration (MIC/MBC) and fractionated inhibitory concentration (FIC) of EGCG and FOSFO against Enterococcus faecalis, Actinomyces israelii, Streptococcus mutans, and Fusobacterium nucleatum. Monospecies and multispecies biofilms with those bacteria formed in polystyrene microplates and in radicular dentin blocks of bovine teeth were treated with the compounds and control chlorhexidine (CHX) and evaluated by bacterial counts and microscopy analysis. Toxicity effect of the compounds was determined on fibroblasts culture by methyl tetrazolium assays. Results: The combination of EGCG + FOSFO demonstrated synergism against all bacterial species, with an FIC index ranging from 0.35 to 0.5. At the MIC/FIC concentrations, EGCG, FOSFO, and EGCG+FOSFO were not toxic to fibroblasts. EGCG+FOSFO significantly reduced monospecies biofilms of E. faecalis and A. israelli, whereas S. mutans and F. nucleatum biofilms were eliminated by all compounds. Scanning electron microscopy of multispecies biofilms treated with EGCG, EGCG+FOSFO, and CHX at 100x MIC showed evident biofilm disorganization and substantial reduction of extracellular matrix. Confocal microscopy observed a significant reduction of multispecies biofilms formed in dentin tubules with 84.85%, 78.49%, and 50.6% of dead cells for EGCG+FOSFO, EGCG, and CHX at 100x MIC, respectively. Conclusion: EGCG and fosfomycin showed a synergistic effect against biofilms of oral pathogens related to root canal infections without causing cytotoxicity.Universidade de São Paulo. Faculdade de Odontologia de Bauru2023-03-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/21003010.1590/1678-7757-2022-0282 Journal of Applied Oral Science; Vol. 31 (2023); e20220282Journal of Applied Oral Science; Vol. 31 (2023); e20220282Journal of Applied Oral Science; v. 31 (2023); e202202821678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/210030/192546Copyright (c) 2023 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDuque, CristianeSouza, Amanda Caselato AndolfattoAida, Kelly LimiPereira, Jesse AugustoCaiaffa, Karina SampaioSantos, Vanessa Rodrigues dosCosme-Silva, LeopoldoPrakki, Anuradha2023-03-29T19:02:20Zoai:revistas.usp.br:article/210030Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2023-03-29T19:02:20Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections |
title |
Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections |
spellingShingle |
Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections Duque, Cristiane Epigallocatechin gallate Fosfomycin Drug synergism Biofilms Cytotoxicity tests |
title_short |
Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections |
title_full |
Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections |
title_fullStr |
Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections |
title_full_unstemmed |
Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections |
title_sort |
Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections |
author |
Duque, Cristiane |
author_facet |
Duque, Cristiane Souza, Amanda Caselato Andolfatto Aida, Kelly Limi Pereira, Jesse Augusto Caiaffa, Karina Sampaio Santos, Vanessa Rodrigues dos Cosme-Silva, Leopoldo Prakki, Anuradha |
author_role |
author |
author2 |
Souza, Amanda Caselato Andolfatto Aida, Kelly Limi Pereira, Jesse Augusto Caiaffa, Karina Sampaio Santos, Vanessa Rodrigues dos Cosme-Silva, Leopoldo Prakki, Anuradha |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Duque, Cristiane Souza, Amanda Caselato Andolfatto Aida, Kelly Limi Pereira, Jesse Augusto Caiaffa, Karina Sampaio Santos, Vanessa Rodrigues dos Cosme-Silva, Leopoldo Prakki, Anuradha |
dc.subject.por.fl_str_mv |
Epigallocatechin gallate Fosfomycin Drug synergism Biofilms Cytotoxicity tests |
topic |
Epigallocatechin gallate Fosfomycin Drug synergism Biofilms Cytotoxicity tests |
description |
Objective: This study aimed to evaluate the cytotoxicity and synergistic effect of epigallocatechin gallate (EGCG) and fosfomycin (FOSFO) on biofilms of oral bacteria associated with endodontic infections. Methodology: This study determined minimum inhibitory and bactericidal concentration (MIC/MBC) and fractionated inhibitory concentration (FIC) of EGCG and FOSFO against Enterococcus faecalis, Actinomyces israelii, Streptococcus mutans, and Fusobacterium nucleatum. Monospecies and multispecies biofilms with those bacteria formed in polystyrene microplates and in radicular dentin blocks of bovine teeth were treated with the compounds and control chlorhexidine (CHX) and evaluated by bacterial counts and microscopy analysis. Toxicity effect of the compounds was determined on fibroblasts culture by methyl tetrazolium assays. Results: The combination of EGCG + FOSFO demonstrated synergism against all bacterial species, with an FIC index ranging from 0.35 to 0.5. At the MIC/FIC concentrations, EGCG, FOSFO, and EGCG+FOSFO were not toxic to fibroblasts. EGCG+FOSFO significantly reduced monospecies biofilms of E. faecalis and A. israelli, whereas S. mutans and F. nucleatum biofilms were eliminated by all compounds. Scanning electron microscopy of multispecies biofilms treated with EGCG, EGCG+FOSFO, and CHX at 100x MIC showed evident biofilm disorganization and substantial reduction of extracellular matrix. Confocal microscopy observed a significant reduction of multispecies biofilms formed in dentin tubules with 84.85%, 78.49%, and 50.6% of dead cells for EGCG+FOSFO, EGCG, and CHX at 100x MIC, respectively. Conclusion: EGCG and fosfomycin showed a synergistic effect against biofilms of oral pathogens related to root canal infections without causing cytotoxicity. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/210030 10.1590/1678-7757-2022-0282 |
url |
https://www.revistas.usp.br/jaos/article/view/210030 |
identifier_str_mv |
10.1590/1678-7757-2022-0282 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/210030/192546 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 31 (2023); e20220282 Journal of Applied Oral Science; Vol. 31 (2023); e20220282 Journal of Applied Oral Science; v. 31 (2023); e20220282 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221683536101376 |