Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections

Detalhes bibliográficos
Autor(a) principal: Duque, Cristiane
Data de Publicação: 2023
Outros Autores: Souza, Amanda Caselato Andolfatto, Aida, Kelly Limi, Pereira, Jesse Augusto, Caiaffa, Karina Sampaio, Santos, Vanessa Rodrigues dos, Cosme-Silva, Leopoldo, Prakki, Anuradha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/210030
Resumo: Objective: This study aimed to evaluate the cytotoxicity and synergistic effect of epigallocatechin gallate (EGCG) and fosfomycin (FOSFO) on biofilms of oral bacteria associated with endodontic infections. Methodology: This study determined minimum inhibitory and bactericidal concentration (MIC/MBC) and fractionated inhibitory concentration (FIC) of EGCG and FOSFO against Enterococcus faecalis, Actinomyces israelii, Streptococcus mutans, and Fusobacterium nucleatum. Monospecies and multispecies biofilms with those bacteria formed in polystyrene microplates and in radicular dentin blocks of bovine teeth were treated with the compounds and control chlorhexidine (CHX) and evaluated by bacterial counts and microscopy analysis. Toxicity effect of the compounds was determined on fibroblasts culture by methyl tetrazolium assays. Results: The combination of EGCG + FOSFO demonstrated synergism against all bacterial species, with an FIC index ranging from 0.35 to 0.5. At the MIC/FIC concentrations, EGCG, FOSFO, and EGCG+FOSFO were not toxic to fibroblasts. EGCG+FOSFO significantly reduced monospecies biofilms of E. faecalis and A. israelli, whereas S. mutans and F. nucleatum biofilms were eliminated by all compounds. Scanning electron microscopy of multispecies biofilms treated with EGCG, EGCG+FOSFO, and CHX at 100x MIC showed evident biofilm disorganization and substantial reduction of extracellular matrix. Confocal microscopy observed a significant reduction of multispecies biofilms formed in dentin tubules with 84.85%, 78.49%, and 50.6% of dead cells for EGCG+FOSFO, EGCG, and CHX at 100x MIC, respectively. Conclusion: EGCG and fosfomycin showed a synergistic effect against biofilms of oral pathogens related to root canal infections without causing cytotoxicity.
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spelling Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infectionsEpigallocatechin gallateFosfomycinDrug synergismBiofilmsCytotoxicity testsObjective: This study aimed to evaluate the cytotoxicity and synergistic effect of epigallocatechin gallate (EGCG) and fosfomycin (FOSFO) on biofilms of oral bacteria associated with endodontic infections. Methodology: This study determined minimum inhibitory and bactericidal concentration (MIC/MBC) and fractionated inhibitory concentration (FIC) of EGCG and FOSFO against Enterococcus faecalis, Actinomyces israelii, Streptococcus mutans, and Fusobacterium nucleatum. Monospecies and multispecies biofilms with those bacteria formed in polystyrene microplates and in radicular dentin blocks of bovine teeth were treated with the compounds and control chlorhexidine (CHX) and evaluated by bacterial counts and microscopy analysis. Toxicity effect of the compounds was determined on fibroblasts culture by methyl tetrazolium assays. Results: The combination of EGCG + FOSFO demonstrated synergism against all bacterial species, with an FIC index ranging from 0.35 to 0.5. At the MIC/FIC concentrations, EGCG, FOSFO, and EGCG+FOSFO were not toxic to fibroblasts. EGCG+FOSFO significantly reduced monospecies biofilms of E. faecalis and A. israelli, whereas S. mutans and F. nucleatum biofilms were eliminated by all compounds. Scanning electron microscopy of multispecies biofilms treated with EGCG, EGCG+FOSFO, and CHX at 100x MIC showed evident biofilm disorganization and substantial reduction of extracellular matrix. Confocal microscopy observed a significant reduction of multispecies biofilms formed in dentin tubules with 84.85%, 78.49%, and 50.6% of dead cells for EGCG+FOSFO, EGCG, and CHX at 100x MIC, respectively. Conclusion: EGCG and fosfomycin showed a synergistic effect against biofilms of oral pathogens related to root canal infections without causing cytotoxicity.Universidade de São Paulo. Faculdade de Odontologia de Bauru2023-03-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/21003010.1590/1678-7757-2022-0282 Journal of Applied Oral Science; Vol. 31 (2023); e20220282Journal of Applied Oral Science; Vol. 31 (2023); e20220282Journal of Applied Oral Science; v. 31 (2023); e202202821678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/210030/192546Copyright (c) 2023 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDuque, CristianeSouza, Amanda Caselato AndolfattoAida, Kelly LimiPereira, Jesse AugustoCaiaffa, Karina SampaioSantos, Vanessa Rodrigues dosCosme-Silva, LeopoldoPrakki, Anuradha2023-03-29T19:02:20Zoai:revistas.usp.br:article/210030Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2023-03-29T19:02:20Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections
title Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections
spellingShingle Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections
Duque, Cristiane
Epigallocatechin gallate
Fosfomycin
Drug synergism
Biofilms
Cytotoxicity tests
title_short Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections
title_full Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections
title_fullStr Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections
title_full_unstemmed Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections
title_sort Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections
author Duque, Cristiane
author_facet Duque, Cristiane
Souza, Amanda Caselato Andolfatto
Aida, Kelly Limi
Pereira, Jesse Augusto
Caiaffa, Karina Sampaio
Santos, Vanessa Rodrigues dos
Cosme-Silva, Leopoldo
Prakki, Anuradha
author_role author
author2 Souza, Amanda Caselato Andolfatto
Aida, Kelly Limi
Pereira, Jesse Augusto
Caiaffa, Karina Sampaio
Santos, Vanessa Rodrigues dos
Cosme-Silva, Leopoldo
Prakki, Anuradha
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Duque, Cristiane
Souza, Amanda Caselato Andolfatto
Aida, Kelly Limi
Pereira, Jesse Augusto
Caiaffa, Karina Sampaio
Santos, Vanessa Rodrigues dos
Cosme-Silva, Leopoldo
Prakki, Anuradha
dc.subject.por.fl_str_mv Epigallocatechin gallate
Fosfomycin
Drug synergism
Biofilms
Cytotoxicity tests
topic Epigallocatechin gallate
Fosfomycin
Drug synergism
Biofilms
Cytotoxicity tests
description Objective: This study aimed to evaluate the cytotoxicity and synergistic effect of epigallocatechin gallate (EGCG) and fosfomycin (FOSFO) on biofilms of oral bacteria associated with endodontic infections. Methodology: This study determined minimum inhibitory and bactericidal concentration (MIC/MBC) and fractionated inhibitory concentration (FIC) of EGCG and FOSFO against Enterococcus faecalis, Actinomyces israelii, Streptococcus mutans, and Fusobacterium nucleatum. Monospecies and multispecies biofilms with those bacteria formed in polystyrene microplates and in radicular dentin blocks of bovine teeth were treated with the compounds and control chlorhexidine (CHX) and evaluated by bacterial counts and microscopy analysis. Toxicity effect of the compounds was determined on fibroblasts culture by methyl tetrazolium assays. Results: The combination of EGCG + FOSFO demonstrated synergism against all bacterial species, with an FIC index ranging from 0.35 to 0.5. At the MIC/FIC concentrations, EGCG, FOSFO, and EGCG+FOSFO were not toxic to fibroblasts. EGCG+FOSFO significantly reduced monospecies biofilms of E. faecalis and A. israelli, whereas S. mutans and F. nucleatum biofilms were eliminated by all compounds. Scanning electron microscopy of multispecies biofilms treated with EGCG, EGCG+FOSFO, and CHX at 100x MIC showed evident biofilm disorganization and substantial reduction of extracellular matrix. Confocal microscopy observed a significant reduction of multispecies biofilms formed in dentin tubules with 84.85%, 78.49%, and 50.6% of dead cells for EGCG+FOSFO, EGCG, and CHX at 100x MIC, respectively. Conclusion: EGCG and fosfomycin showed a synergistic effect against biofilms of oral pathogens related to root canal infections without causing cytotoxicity.
publishDate 2023
dc.date.none.fl_str_mv 2023-03-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/210030
10.1590/1678-7757-2022-0282
url https://www.revistas.usp.br/jaos/article/view/210030
identifier_str_mv 10.1590/1678-7757-2022-0282
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/210030/192546
dc.rights.driver.fl_str_mv Copyright (c) 2023 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 31 (2023); e20220282
Journal of Applied Oral Science; Vol. 31 (2023); e20220282
Journal of Applied Oral Science; v. 31 (2023); e20220282
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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