Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/101002 |
Resumo: | There are several age-related microscopic changes in the salivary glands, including the increase in the number of duct-like structures (DLS). However, the true origin and the phenotype of the DLS are not known. Objective To evaluate the phenotype and the cell proliferation index of the DLS of human sublingual glands. Material and Methods Sixty sublingual glands obtained from human cadavers were divided into two groups - 0-30 and 61-90 years old. The phenotype was estimated by immunostaining for cytokeratin 19 (CK 19) and the S-100 protein as well as by the presence of mucin and glycogen. The cell proliferation index was determined by the Ki-67 antibody. The histochemical techniques used periodic acid-Schiff (PAS) and Alcian Blue. In each captured microscopic field, the DLS were counted to establish a percentage for the staining profile. The statistical analysis was accomplished using Student's t-test, the Mann-Whitney test and Pearson's correlation coefficient (p |
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Journal of applied oral science (Online) |
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Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study There are several age-related microscopic changes in the salivary glands, including the increase in the number of duct-like structures (DLS). However, the true origin and the phenotype of the DLS are not known. Objective To evaluate the phenotype and the cell proliferation index of the DLS of human sublingual glands. Material and Methods Sixty sublingual glands obtained from human cadavers were divided into two groups - 0-30 and 61-90 years old. The phenotype was estimated by immunostaining for cytokeratin 19 (CK 19) and the S-100 protein as well as by the presence of mucin and glycogen. The cell proliferation index was determined by the Ki-67 antibody. The histochemical techniques used periodic acid-Schiff (PAS) and Alcian Blue. In each captured microscopic field, the DLS were counted to establish a percentage for the staining profile. The statistical analysis was accomplished using Student's t-test, the Mann-Whitney test and Pearson's correlation coefficient (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/10100210.1590/1678-775720140349Journal of Applied Oral Science; Vol. 23 No. 3 (2015); 255-264Journal of Applied Oral Science; Vol. 23 Núm. 3 (2015); 255-264Journal of Applied Oral Science; v. 23 n. 3 (2015); 255-2641678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/101002/99666Copyright (c) 2015 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessTOLENTINO, Elen de Souza TEIXEIRA, Cleverson Soares AZEVEDO-ALANIS, Luciana Reis HONÓRIO, Heitor Marques DAMANTE, José Humberto 2015-07-28T17:30:16Zoai:revistas.usp.br:article/101002Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2015-07-28T17:30:16Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study |
title |
Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study |
spellingShingle |
Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study TOLENTINO, Elen de Souza |
title_short |
Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study |
title_full |
Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study |
title_fullStr |
Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study |
title_full_unstemmed |
Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study |
title_sort |
Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study |
author |
TOLENTINO, Elen de Souza |
author_facet |
TOLENTINO, Elen de Souza TEIXEIRA, Cleverson Soares AZEVEDO-ALANIS, Luciana Reis HONÓRIO, Heitor Marques DAMANTE, José Humberto |
author_role |
author |
author2 |
TEIXEIRA, Cleverson Soares AZEVEDO-ALANIS, Luciana Reis HONÓRIO, Heitor Marques DAMANTE, José Humberto |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
TOLENTINO, Elen de Souza TEIXEIRA, Cleverson Soares AZEVEDO-ALANIS, Luciana Reis HONÓRIO, Heitor Marques DAMANTE, José Humberto |
description |
There are several age-related microscopic changes in the salivary glands, including the increase in the number of duct-like structures (DLS). However, the true origin and the phenotype of the DLS are not known. Objective To evaluate the phenotype and the cell proliferation index of the DLS of human sublingual glands. Material and Methods Sixty sublingual glands obtained from human cadavers were divided into two groups - 0-30 and 61-90 years old. The phenotype was estimated by immunostaining for cytokeratin 19 (CK 19) and the S-100 protein as well as by the presence of mucin and glycogen. The cell proliferation index was determined by the Ki-67 antibody. The histochemical techniques used periodic acid-Schiff (PAS) and Alcian Blue. In each captured microscopic field, the DLS were counted to establish a percentage for the staining profile. The statistical analysis was accomplished using Student's t-test, the Mann-Whitney test and Pearson's correlation coefficient (p |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/101002 10.1590/1678-775720140349 |
url |
https://www.revistas.usp.br/jaos/article/view/101002 |
identifier_str_mv |
10.1590/1678-775720140349 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/101002/99666 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 23 No. 3 (2015); 255-264 Journal of Applied Oral Science; Vol. 23 Núm. 3 (2015); 255-264 Journal of Applied Oral Science; v. 23 n. 3 (2015); 255-264 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221678771372032 |