Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study

Detalhes bibliográficos
Autor(a) principal: TOLENTINO, Elen de Souza
Data de Publicação: 2015
Outros Autores: TEIXEIRA, Cleverson Soares, AZEVEDO-ALANIS, Luciana Reis, HONÓRIO, Heitor Marques, DAMANTE, José Humberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/101002
Resumo: There are several age-related microscopic changes in the salivary glands, including the increase in the number of duct-like structures (DLS). However, the true origin and the phenotype of the DLS are not known. Objective To evaluate the phenotype and the cell proliferation index of the DLS of human sublingual glands. Material and Methods Sixty sublingual glands obtained from human cadavers were divided into two groups - 0-30 and 61-90 years old. The phenotype was estimated by immunostaining for cytokeratin 19 (CK 19) and the S-100 protein as well as by the presence of mucin and glycogen. The cell proliferation index was determined by the Ki-67 antibody. The histochemical techniques used periodic acid-Schiff (PAS) and Alcian Blue. In each captured microscopic field, the DLS were counted to establish a percentage for the staining profile. The statistical analysis was accomplished using Student's t-test, the Mann-Whitney test and Pearson's correlation coefficient (p
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spelling Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study There are several age-related microscopic changes in the salivary glands, including the increase in the number of duct-like structures (DLS). However, the true origin and the phenotype of the DLS are not known. Objective To evaluate the phenotype and the cell proliferation index of the DLS of human sublingual glands. Material and Methods Sixty sublingual glands obtained from human cadavers were divided into two groups - 0-30 and 61-90 years old. The phenotype was estimated by immunostaining for cytokeratin 19 (CK 19) and the S-100 protein as well as by the presence of mucin and glycogen. The cell proliferation index was determined by the Ki-67 antibody. The histochemical techniques used periodic acid-Schiff (PAS) and Alcian Blue. In each captured microscopic field, the DLS were counted to establish a percentage for the staining profile. The statistical analysis was accomplished using Student's t-test, the Mann-Whitney test and Pearson's correlation coefficient (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/10100210.1590/1678-775720140349Journal of Applied Oral Science; Vol. 23 No. 3 (2015); 255-264Journal of Applied Oral Science; Vol. 23 Núm. 3 (2015); 255-264Journal of Applied Oral Science; v. 23 n. 3 (2015); 255-2641678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/101002/99666Copyright (c) 2015 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessTOLENTINO, Elen de Souza TEIXEIRA, Cleverson Soares AZEVEDO-ALANIS, Luciana Reis HONÓRIO, Heitor Marques DAMANTE, José Humberto 2015-07-28T17:30:16Zoai:revistas.usp.br:article/101002Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2015-07-28T17:30:16Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study
title Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study
spellingShingle Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study
TOLENTINO, Elen de Souza
title_short Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study
title_full Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study
title_fullStr Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study
title_full_unstemmed Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study
title_sort Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study
author TOLENTINO, Elen de Souza
author_facet TOLENTINO, Elen de Souza
TEIXEIRA, Cleverson Soares
AZEVEDO-ALANIS, Luciana Reis
HONÓRIO, Heitor Marques
DAMANTE, José Humberto
author_role author
author2 TEIXEIRA, Cleverson Soares
AZEVEDO-ALANIS, Luciana Reis
HONÓRIO, Heitor Marques
DAMANTE, José Humberto
author2_role author
author
author
author
dc.contributor.author.fl_str_mv TOLENTINO, Elen de Souza
TEIXEIRA, Cleverson Soares
AZEVEDO-ALANIS, Luciana Reis
HONÓRIO, Heitor Marques
DAMANTE, José Humberto
description There are several age-related microscopic changes in the salivary glands, including the increase in the number of duct-like structures (DLS). However, the true origin and the phenotype of the DLS are not known. Objective To evaluate the phenotype and the cell proliferation index of the DLS of human sublingual glands. Material and Methods Sixty sublingual glands obtained from human cadavers were divided into two groups - 0-30 and 61-90 years old. The phenotype was estimated by immunostaining for cytokeratin 19 (CK 19) and the S-100 protein as well as by the presence of mucin and glycogen. The cell proliferation index was determined by the Ki-67 antibody. The histochemical techniques used periodic acid-Schiff (PAS) and Alcian Blue. In each captured microscopic field, the DLS were counted to establish a percentage for the staining profile. The statistical analysis was accomplished using Student's t-test, the Mann-Whitney test and Pearson's correlation coefficient (p
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/101002
10.1590/1678-775720140349
url https://www.revistas.usp.br/jaos/article/view/101002
identifier_str_mv 10.1590/1678-775720140349
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/101002/99666
dc.rights.driver.fl_str_mv Copyright (c) 2015 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2015 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 23 No. 3 (2015); 255-264
Journal of Applied Oral Science; Vol. 23 Núm. 3 (2015); 255-264
Journal of Applied Oral Science; v. 23 n. 3 (2015); 255-264
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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