Nail and bone surface as indicators of acute exposure to fluoride in rats

Detalhes bibliográficos
Autor(a) principal: Buzalaf, Marília Afonso Rabelo
Data de Publicação: 2004
Outros Autores: Linardi, Myrna Maria, Carvalho, Juliane Guimarães de, Cardoso, Vanessa Eid da Silva, Cury, Jaime Aparecido
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/3222
Resumo: This study evaluated the use of nails and bone surfaces as indicators of acute exposure to fluoride in rats. Six groups (n=10/grp), aged 70 days, received, by gastrogavage, single doses of sodium fluoride containing 10, 35, 45, 60, 75 and 90 mg fluoride/kg body weight. The control group received deionized water. Two hours after fluoride administration, the rats were killed and their plasma, nails (halves near to the growth end) and femur were collected. Nail and plasma fluoride concentrations were analyzed with the electrode following HMDS-facilitated diffusion. Femur surface fluoride was removed from a circular area (4.52 mm²) by immersion in 0.5M HCl for 15 seconds, buffered with TISAB and analyzed with the electrode. Data were analyzed by ANOVA and Tukey's test (p
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spelling Nail and bone surface as indicators of acute exposure to fluoride in rats Unha e superfície óssea como indicadores da exposição aguda em ratos Exposição agudaBiomarcadoresOssoFlúorUnhasAcute exposureBiomarkersBoneFluorideNails This study evaluated the use of nails and bone surfaces as indicators of acute exposure to fluoride in rats. Six groups (n=10/grp), aged 70 days, received, by gastrogavage, single doses of sodium fluoride containing 10, 35, 45, 60, 75 and 90 mg fluoride/kg body weight. The control group received deionized water. Two hours after fluoride administration, the rats were killed and their plasma, nails (halves near to the growth end) and femur were collected. Nail and plasma fluoride concentrations were analyzed with the electrode following HMDS-facilitated diffusion. Femur surface fluoride was removed from a circular area (4.52 mm²) by immersion in 0.5M HCl for 15 seconds, buffered with TISAB and analyzed with the electrode. Data were analyzed by ANOVA and Tukey's test (p Este estudo avaliou o uso de unhas e superfícies ósseas como indicadores da exposição aguda ao flúor em ratos. Seis grupos (n=10/grp), com idade de 70 dias, receberam, por gastro-gavagem, doses únicas de fluoreto de sódio contendo 10, 35, 45, 60, 75 e 90 mg flúor/kg peso corpóreo. O grupo controle recebeu água deionizada. Duas horas após a administração de flúor, os ratos foram mortos e seu plasma, unhas (metades próximas à porção de crescimento) e fêmur foram coletados. As concentrações de flúor na unha e plasma foram analisadas com o eletrodo após difusão facilitada por HMDS. O flúor da superfície do fêmur foi removida de uma área circular (4,52 mm²) por imersão em HCl 0,5 M por 15 segundos, tamponado com TISAB (contendo NaOH) e analisado com o eletrodo. Os dados foram analisados por ANOVA e teste de Tukey (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2004-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/322210.1590/S1678-77572004000400006Journal of Applied Oral Science; Vol. 12 No. 4 (2004); 285-289 Journal of Applied Oral Science; Vol. 12 Núm. 4 (2004); 285-289 Journal of Applied Oral Science; v. 12 n. 4 (2004); 285-289 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3222/3912Copyright (c) 2004 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessBuzalaf, Marília Afonso RabeloLinardi, Myrna MariaCarvalho, Juliane Guimarães deCardoso, Vanessa Eid da SilvaCury, Jaime Aparecido2012-04-27T11:48:38Zoai:revistas.usp.br:article/3222Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T11:48:38Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Nail and bone surface as indicators of acute exposure to fluoride in rats
Unha e superfície óssea como indicadores da exposição aguda em ratos
title Nail and bone surface as indicators of acute exposure to fluoride in rats
spellingShingle Nail and bone surface as indicators of acute exposure to fluoride in rats
Buzalaf, Marília Afonso Rabelo
Exposição aguda
Biomarcadores
Osso
Flúor
Unhas
Acute exposure
Biomarkers
Bone
Fluoride
Nails
title_short Nail and bone surface as indicators of acute exposure to fluoride in rats
title_full Nail and bone surface as indicators of acute exposure to fluoride in rats
title_fullStr Nail and bone surface as indicators of acute exposure to fluoride in rats
title_full_unstemmed Nail and bone surface as indicators of acute exposure to fluoride in rats
title_sort Nail and bone surface as indicators of acute exposure to fluoride in rats
author Buzalaf, Marília Afonso Rabelo
author_facet Buzalaf, Marília Afonso Rabelo
Linardi, Myrna Maria
Carvalho, Juliane Guimarães de
Cardoso, Vanessa Eid da Silva
Cury, Jaime Aparecido
author_role author
author2 Linardi, Myrna Maria
Carvalho, Juliane Guimarães de
Cardoso, Vanessa Eid da Silva
Cury, Jaime Aparecido
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Buzalaf, Marília Afonso Rabelo
Linardi, Myrna Maria
Carvalho, Juliane Guimarães de
Cardoso, Vanessa Eid da Silva
Cury, Jaime Aparecido
dc.subject.por.fl_str_mv Exposição aguda
Biomarcadores
Osso
Flúor
Unhas
Acute exposure
Biomarkers
Bone
Fluoride
Nails
topic Exposição aguda
Biomarcadores
Osso
Flúor
Unhas
Acute exposure
Biomarkers
Bone
Fluoride
Nails
description This study evaluated the use of nails and bone surfaces as indicators of acute exposure to fluoride in rats. Six groups (n=10/grp), aged 70 days, received, by gastrogavage, single doses of sodium fluoride containing 10, 35, 45, 60, 75 and 90 mg fluoride/kg body weight. The control group received deionized water. Two hours after fluoride administration, the rats were killed and their plasma, nails (halves near to the growth end) and femur were collected. Nail and plasma fluoride concentrations were analyzed with the electrode following HMDS-facilitated diffusion. Femur surface fluoride was removed from a circular area (4.52 mm²) by immersion in 0.5M HCl for 15 seconds, buffered with TISAB and analyzed with the electrode. Data were analyzed by ANOVA and Tukey's test (p
publishDate 2004
dc.date.none.fl_str_mv 2004-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3222
10.1590/S1678-77572004000400006
url https://www.revistas.usp.br/jaos/article/view/3222
identifier_str_mv 10.1590/S1678-77572004000400006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3222/3912
dc.rights.driver.fl_str_mv Copyright (c) 2004 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2004 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 12 No. 4 (2004); 285-289
Journal of Applied Oral Science; Vol. 12 Núm. 4 (2004); 285-289
Journal of Applied Oral Science; v. 12 n. 4 (2004); 285-289
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
_version_ 1800221671416659968

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