Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds

Detalhes bibliográficos
Autor(a) principal: Lim, Eun-su
Data de Publicação: 2016
Outros Autores: Lim, Myung-Jin, Min, Kyung-San, Kwon, Young-Sun, Hwang, Yun-Chan, Yu, Mi-Kyung, Hong, Chan-Ui, Lee, Kwang-Won
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/112893
Resumo: Objective The purpose of this study was to investigate the biological effects of epicatechin (ECN), a crosslinking agent, on human dental pulp cells (hDPCs) cultured in collagen scaffolds. Material and Method To evaluate the effects of ECN on the proliferation of hDPCs, cell counting was performed using optical and fluorescent microscopy. Measurements of alkaline phosphatase (ALP) activity, alizarin red staining, and real-time polymerase chain reactions were performed to assess odontogenic differentiation. The compressive strength and setting time of collagen scaffolds containing ECN were measured. Differential scanning calorimetry was performed to analyze the thermal behavior of collagen in the presence of ECN. Results Epicatechin increased ALP activity, mineralized nodule formation, and the mRNA expression of dentin sialophosphoprotein (DSPP), a specific odontogenic-related marker. Furthermore, ECN upregulated the expression of DSPP in hDPCs cultured in collagen scaffolds. Epicatechin activated the extracellular signal-regulated kinase (ERK) and the treatment with an ERK inhibitor (U0126) blocked the expression of DSPP. The compressive strength was increased and the setting time was shortened in a dose-dependent manner. The number of cells cultured in the ECN-treated collagen scaffolds was significantly increased compared to the cells in the untreated control group. Conclusions Our results revealed that ECN promoted the proliferation and differentiation of hDPCs. Furthermore, the differentiation was regulated by the ERK signaling pathway. Changes in mechanical properties are related to cell fate, including proliferation and differentiation. Therefore, our study suggests the ECN treatment might be desirable for dentin-pulp complex regeneration.
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spelling Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds Objective The purpose of this study was to investigate the biological effects of epicatechin (ECN), a crosslinking agent, on human dental pulp cells (hDPCs) cultured in collagen scaffolds. Material and Method To evaluate the effects of ECN on the proliferation of hDPCs, cell counting was performed using optical and fluorescent microscopy. Measurements of alkaline phosphatase (ALP) activity, alizarin red staining, and real-time polymerase chain reactions were performed to assess odontogenic differentiation. The compressive strength and setting time of collagen scaffolds containing ECN were measured. Differential scanning calorimetry was performed to analyze the thermal behavior of collagen in the presence of ECN. Results Epicatechin increased ALP activity, mineralized nodule formation, and the mRNA expression of dentin sialophosphoprotein (DSPP), a specific odontogenic-related marker. Furthermore, ECN upregulated the expression of DSPP in hDPCs cultured in collagen scaffolds. Epicatechin activated the extracellular signal-regulated kinase (ERK) and the treatment with an ERK inhibitor (U0126) blocked the expression of DSPP. The compressive strength was increased and the setting time was shortened in a dose-dependent manner. The number of cells cultured in the ECN-treated collagen scaffolds was significantly increased compared to the cells in the untreated control group. Conclusions Our results revealed that ECN promoted the proliferation and differentiation of hDPCs. Furthermore, the differentiation was regulated by the ERK signaling pathway. Changes in mechanical properties are related to cell fate, including proliferation and differentiation. Therefore, our study suggests the ECN treatment might be desirable for dentin-pulp complex regeneration. Universidade de São Paulo. Faculdade de Odontologia de Bauru2016-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/11289310.1590/1678-775720150383Journal of Applied Oral Science; Vol. 24 No. 1 (2016); 76-84Journal of Applied Oral Science; Vol. 24 Núm. 1 (2016); 76-84Journal of Applied Oral Science; v. 24 n. 1 (2016); 76-841678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/112893/110837Copyright (c) 2016 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessLim, Eun-su Lim, Myung-Jin Min, Kyung-San Kwon, Young-Sun Hwang, Yun-Chan Yu, Mi-Kyung Hong, Chan-Ui Lee, Kwang-Won 2016-03-23T18:16:02Zoai:revistas.usp.br:article/112893Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2016-03-23T18:16:02Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds
title Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds
spellingShingle Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds
Lim, Eun-su
title_short Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds
title_full Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds
title_fullStr Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds
title_full_unstemmed Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds
title_sort Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds
author Lim, Eun-su
author_facet Lim, Eun-su
Lim, Myung-Jin
Min, Kyung-San
Kwon, Young-Sun
Hwang, Yun-Chan
Yu, Mi-Kyung
Hong, Chan-Ui
Lee, Kwang-Won
author_role author
author2 Lim, Myung-Jin
Min, Kyung-San
Kwon, Young-Sun
Hwang, Yun-Chan
Yu, Mi-Kyung
Hong, Chan-Ui
Lee, Kwang-Won
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lim, Eun-su
Lim, Myung-Jin
Min, Kyung-San
Kwon, Young-Sun
Hwang, Yun-Chan
Yu, Mi-Kyung
Hong, Chan-Ui
Lee, Kwang-Won
description Objective The purpose of this study was to investigate the biological effects of epicatechin (ECN), a crosslinking agent, on human dental pulp cells (hDPCs) cultured in collagen scaffolds. Material and Method To evaluate the effects of ECN on the proliferation of hDPCs, cell counting was performed using optical and fluorescent microscopy. Measurements of alkaline phosphatase (ALP) activity, alizarin red staining, and real-time polymerase chain reactions were performed to assess odontogenic differentiation. The compressive strength and setting time of collagen scaffolds containing ECN were measured. Differential scanning calorimetry was performed to analyze the thermal behavior of collagen in the presence of ECN. Results Epicatechin increased ALP activity, mineralized nodule formation, and the mRNA expression of dentin sialophosphoprotein (DSPP), a specific odontogenic-related marker. Furthermore, ECN upregulated the expression of DSPP in hDPCs cultured in collagen scaffolds. Epicatechin activated the extracellular signal-regulated kinase (ERK) and the treatment with an ERK inhibitor (U0126) blocked the expression of DSPP. The compressive strength was increased and the setting time was shortened in a dose-dependent manner. The number of cells cultured in the ECN-treated collagen scaffolds was significantly increased compared to the cells in the untreated control group. Conclusions Our results revealed that ECN promoted the proliferation and differentiation of hDPCs. Furthermore, the differentiation was regulated by the ERK signaling pathway. Changes in mechanical properties are related to cell fate, including proliferation and differentiation. Therefore, our study suggests the ECN treatment might be desirable for dentin-pulp complex regeneration.
publishDate 2016
dc.date.none.fl_str_mv 2016-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/112893
10.1590/1678-775720150383
url https://www.revistas.usp.br/jaos/article/view/112893
identifier_str_mv 10.1590/1678-775720150383
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/112893/110837
dc.rights.driver.fl_str_mv Copyright (c) 2016 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2016 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 24 No. 1 (2016); 76-84
Journal of Applied Oral Science; Vol. 24 Núm. 1 (2016); 76-84
Journal of Applied Oral Science; v. 24 n. 1 (2016); 76-84
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
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instname_str Universidade de São Paulo (USP)
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institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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