Root canal contamination or exposure to lipopolysaccharide differentially modulate prostaglandin E2 and leukotriene B4 signaling in apical periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/190139 |
Resumo: | Purpose: To evaluate the kinetics of apical periodontitis development in vivo , induced either by contamination of the root canals by microorganisms from the oral cavity or by inoculation of bacterial lipopolysaccharide (LPS) and the regulation of major enzymes and receptors involved in the arachidonic acid metabolism. Methodology: Apical periodontitis was induced in C57BL6 mice (n=96), by root canal exposure to oral cavity (n=48 teeth) or inoculation of LPS (10 µL of a suspension of 0.1 µg/µL) from E. coli into the root canals (n= 48 teeth). Healthy teeth were used as control (n=48 teeth). After 7, 14, 21 and 28 days the animals were euthanized and tissues removed for histopathological and qRT-PCR analyses. Histological analysis data were analyzed using two-way ANOVA followed by Sidak’s test, and qRT-PCR data using two-way ANOVA followed by Tukey’s test (α=0.05). Results: Contamination by microorganisms led to the development of apical periodontitis, characterized by the recruitment of inflammatory cells and bone tissue resorption, whereas inoculation of LPS induced inflammatory cells recruitment without bone resorption. Both stimuli induced mRNA expression for cyclooxygenase-2 and 5-lipoxygenase enzymes. Expression of prostaglandin E2 and leukotriene B4 cell surface receptors were more stimulated by LPS. Regarding nuclear peroxisome proliferator-activated receptors (PPAR), oral contamination induced the synthesis of mRNA for PPARδ, differently from inoculation of LPS, that induced PPARα and PPARγ expression. Conclusions: Contamination of the root canals by microorganisms from oral cavity induced the development of apical periodontitis differently than by inoculation with LPS, characterized by less bone loss than the first model. Regardless of the model used, it was found a local increase in the synthesis of mRNA for the enzymes 5-lipoxygenase and cyclooxygenase-2 of the arachidonic acid metabolism, as well as in the surface and nuclear receptors for the lipid mediators prostaglandin E2 and leukotriene B4. |
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oai:revistas.usp.br:article/190139 |
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Journal of applied oral science (Online) |
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Root canal contamination or exposure to lipopolysaccharide differentially modulate prostaglandin E2 and leukotriene B4 signaling in apical periodontitisProstaglandin E2Leukotriene B4Apical periodontitisLipopolysaccharideRoot canal contaminationPurpose: To evaluate the kinetics of apical periodontitis development in vivo , induced either by contamination of the root canals by microorganisms from the oral cavity or by inoculation of bacterial lipopolysaccharide (LPS) and the regulation of major enzymes and receptors involved in the arachidonic acid metabolism. Methodology: Apical periodontitis was induced in C57BL6 mice (n=96), by root canal exposure to oral cavity (n=48 teeth) or inoculation of LPS (10 µL of a suspension of 0.1 µg/µL) from E. coli into the root canals (n= 48 teeth). Healthy teeth were used as control (n=48 teeth). After 7, 14, 21 and 28 days the animals were euthanized and tissues removed for histopathological and qRT-PCR analyses. Histological analysis data were analyzed using two-way ANOVA followed by Sidak’s test, and qRT-PCR data using two-way ANOVA followed by Tukey’s test (α=0.05). Results: Contamination by microorganisms led to the development of apical periodontitis, characterized by the recruitment of inflammatory cells and bone tissue resorption, whereas inoculation of LPS induced inflammatory cells recruitment without bone resorption. Both stimuli induced mRNA expression for cyclooxygenase-2 and 5-lipoxygenase enzymes. Expression of prostaglandin E2 and leukotriene B4 cell surface receptors were more stimulated by LPS. Regarding nuclear peroxisome proliferator-activated receptors (PPAR), oral contamination induced the synthesis of mRNA for PPARδ, differently from inoculation of LPS, that induced PPARα and PPARγ expression. Conclusions: Contamination of the root canals by microorganisms from oral cavity induced the development of apical periodontitis differently than by inoculation with LPS, characterized by less bone loss than the first model. Regardless of the model used, it was found a local increase in the synthesis of mRNA for the enzymes 5-lipoxygenase and cyclooxygenase-2 of the arachidonic acid metabolism, as well as in the surface and nuclear receptors for the lipid mediators prostaglandin E2 and leukotriene B4.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/19013910.1590/1678-7757-2019-0699Journal of Applied Oral Science; Vol. 28 (2020); e20190699Journal of Applied Oral Science; Vol. 28 (2020); e20190699Journal of Applied Oral Science; v. 28 (2020); e201906991678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/190139/175534Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPaula-Silva, Francisco Wanderley Garcia Ribeiro-Santos, Fernanda Regina Petean, Igor Bassi Ferreira Manfrin Arnez, Maya FernandaAlmeida-Junior, Luciano Aparecido de Carvalho, Fabrício Kitazono de Silva, Léa Assed Bezerra daFaccioli, Lúcia Helena2021-08-30T13:39:32Zoai:revistas.usp.br:article/190139Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-08-30T13:39:32Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Root canal contamination or exposure to lipopolysaccharide differentially modulate prostaglandin E2 and leukotriene B4 signaling in apical periodontitis |
title |
Root canal contamination or exposure to lipopolysaccharide differentially modulate prostaglandin E2 and leukotriene B4 signaling in apical periodontitis |
spellingShingle |
Root canal contamination or exposure to lipopolysaccharide differentially modulate prostaglandin E2 and leukotriene B4 signaling in apical periodontitis Paula-Silva, Francisco Wanderley Garcia Prostaglandin E2 Leukotriene B4 Apical periodontitis Lipopolysaccharide Root canal contamination |
title_short |
Root canal contamination or exposure to lipopolysaccharide differentially modulate prostaglandin E2 and leukotriene B4 signaling in apical periodontitis |
title_full |
Root canal contamination or exposure to lipopolysaccharide differentially modulate prostaglandin E2 and leukotriene B4 signaling in apical periodontitis |
title_fullStr |
Root canal contamination or exposure to lipopolysaccharide differentially modulate prostaglandin E2 and leukotriene B4 signaling in apical periodontitis |
title_full_unstemmed |
Root canal contamination or exposure to lipopolysaccharide differentially modulate prostaglandin E2 and leukotriene B4 signaling in apical periodontitis |
title_sort |
Root canal contamination or exposure to lipopolysaccharide differentially modulate prostaglandin E2 and leukotriene B4 signaling in apical periodontitis |
author |
Paula-Silva, Francisco Wanderley Garcia |
author_facet |
Paula-Silva, Francisco Wanderley Garcia Ribeiro-Santos, Fernanda Regina Petean, Igor Bassi Ferreira Manfrin Arnez, Maya Fernanda Almeida-Junior, Luciano Aparecido de Carvalho, Fabrício Kitazono de Silva, Léa Assed Bezerra da Faccioli, Lúcia Helena |
author_role |
author |
author2 |
Ribeiro-Santos, Fernanda Regina Petean, Igor Bassi Ferreira Manfrin Arnez, Maya Fernanda Almeida-Junior, Luciano Aparecido de Carvalho, Fabrício Kitazono de Silva, Léa Assed Bezerra da Faccioli, Lúcia Helena |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Paula-Silva, Francisco Wanderley Garcia Ribeiro-Santos, Fernanda Regina Petean, Igor Bassi Ferreira Manfrin Arnez, Maya Fernanda Almeida-Junior, Luciano Aparecido de Carvalho, Fabrício Kitazono de Silva, Léa Assed Bezerra da Faccioli, Lúcia Helena |
dc.subject.por.fl_str_mv |
Prostaglandin E2 Leukotriene B4 Apical periodontitis Lipopolysaccharide Root canal contamination |
topic |
Prostaglandin E2 Leukotriene B4 Apical periodontitis Lipopolysaccharide Root canal contamination |
description |
Purpose: To evaluate the kinetics of apical periodontitis development in vivo , induced either by contamination of the root canals by microorganisms from the oral cavity or by inoculation of bacterial lipopolysaccharide (LPS) and the regulation of major enzymes and receptors involved in the arachidonic acid metabolism. Methodology: Apical periodontitis was induced in C57BL6 mice (n=96), by root canal exposure to oral cavity (n=48 teeth) or inoculation of LPS (10 µL of a suspension of 0.1 µg/µL) from E. coli into the root canals (n= 48 teeth). Healthy teeth were used as control (n=48 teeth). After 7, 14, 21 and 28 days the animals were euthanized and tissues removed for histopathological and qRT-PCR analyses. Histological analysis data were analyzed using two-way ANOVA followed by Sidak’s test, and qRT-PCR data using two-way ANOVA followed by Tukey’s test (α=0.05). Results: Contamination by microorganisms led to the development of apical periodontitis, characterized by the recruitment of inflammatory cells and bone tissue resorption, whereas inoculation of LPS induced inflammatory cells recruitment without bone resorption. Both stimuli induced mRNA expression for cyclooxygenase-2 and 5-lipoxygenase enzymes. Expression of prostaglandin E2 and leukotriene B4 cell surface receptors were more stimulated by LPS. Regarding nuclear peroxisome proliferator-activated receptors (PPAR), oral contamination induced the synthesis of mRNA for PPARδ, differently from inoculation of LPS, that induced PPARα and PPARγ expression. Conclusions: Contamination of the root canals by microorganisms from oral cavity induced the development of apical periodontitis differently than by inoculation with LPS, characterized by less bone loss than the first model. Regardless of the model used, it was found a local increase in the synthesis of mRNA for the enzymes 5-lipoxygenase and cyclooxygenase-2 of the arachidonic acid metabolism, as well as in the surface and nuclear receptors for the lipid mediators prostaglandin E2 and leukotriene B4. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/190139 10.1590/1678-7757-2019-0699 |
url |
https://www.revistas.usp.br/jaos/article/view/190139 |
identifier_str_mv |
10.1590/1678-7757-2019-0699 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/190139/175534 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 28 (2020); e20190699 Journal of Applied Oral Science; Vol. 28 (2020); e20190699 Journal of Applied Oral Science; v. 28 (2020); e20190699 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221681955897344 |