Differential expression of salivary glycoproteins in aggressive and chronic periodontitis

Detalhes bibliográficos
Autor(a) principal: Rocha, Daniela de Morais
Data de Publicação: 2012
Outros Autores: Zenóbio, Elton Gonçalves, Van Dyke, Thomas, Silva, Karine Simões, Costa, Fernando Oliveira, Soares, Rodrigo Villamarim
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/46217
Resumo: OBJECTIVES:The aim of this study was to compare the pattern of secretion and the expression of mucin glycoprotein-2 (MG2) and lactoferrin in individuals with or without periodontitis. MATERIAL AND METHODS: Five individuals with aggressive periodontitis (APG), 5 with generalized chronic periodontitis (CPG) and 5 without periodontitis (CG) were enrolled after informed consent. Non-stimulated and stimulated submandibular and sublingual saliva was collected and samples analyzed by Western blot probed with specific antibodies. RESULTS: Stimulated and non-stimulated salivary flow rates did not differ among groups. Western blot analysis revealed that stimulation led to: an increase in MG2 expression in all groups, and to lactoferrin expression in APG and CPG. In non-stimulated saliva, CG exhibited the highest expression of both glycoproteins. In stimulated saliva, CG exhibited the highest expression of MG2, whereas APG the highest of lactoferrin. CONCLUSIONS: The pattern of secretion of MG2 and lactoferrin in health and disease is complex. Although the present study analyzed samples from a limited number of participants, the reduced expression of MG2 and lactoferrin in APG and CPG under non-stimulated condition, the predominant circumstance of salivary secretion during the day, suggests that these salivary constituents may play a role in the etiopathogenesis of these diseases.
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spelling Differential expression of salivary glycoproteins in aggressive and chronic periodontitisSubmandibular glandSublingual glandSalivaInnate immunityPeriodontitisGlycoproteinsOBJECTIVES:The aim of this study was to compare the pattern of secretion and the expression of mucin glycoprotein-2 (MG2) and lactoferrin in individuals with or without periodontitis. MATERIAL AND METHODS: Five individuals with aggressive periodontitis (APG), 5 with generalized chronic periodontitis (CPG) and 5 without periodontitis (CG) were enrolled after informed consent. Non-stimulated and stimulated submandibular and sublingual saliva was collected and samples analyzed by Western blot probed with specific antibodies. RESULTS: Stimulated and non-stimulated salivary flow rates did not differ among groups. Western blot analysis revealed that stimulation led to: an increase in MG2 expression in all groups, and to lactoferrin expression in APG and CPG. In non-stimulated saliva, CG exhibited the highest expression of both glycoproteins. In stimulated saliva, CG exhibited the highest expression of MG2, whereas APG the highest of lactoferrin. CONCLUSIONS: The pattern of secretion of MG2 and lactoferrin in health and disease is complex. Although the present study analyzed samples from a limited number of participants, the reduced expression of MG2 and lactoferrin in APG and CPG under non-stimulated condition, the predominant circumstance of salivary secretion during the day, suggests that these salivary constituents may play a role in the etiopathogenesis of these diseases.Universidade de São Paulo. Faculdade de Odontologia de Bauru2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/4621710.1590/S1678-77572012000200010Journal of Applied Oral Science; Vol. 20 No. 2 (2012); 180-185Journal of Applied Oral Science; Vol. 20 Núm. 2 (2012); 180-185Journal of Applied Oral Science; v. 20 n. 2 (2012); 180-1851678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/46217/49839Copyright (c) 2012 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessRocha, Daniela de MoraisZenóbio, Elton GonçalvesVan Dyke, ThomasSilva, Karine SimõesCosta, Fernando OliveiraSoares, Rodrigo Villamarim2012-10-18T19:34:56Zoai:revistas.usp.br:article/46217Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-10-18T19:34:56Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Differential expression of salivary glycoproteins in aggressive and chronic periodontitis
title Differential expression of salivary glycoproteins in aggressive and chronic periodontitis
spellingShingle Differential expression of salivary glycoproteins in aggressive and chronic periodontitis
Rocha, Daniela de Morais
Submandibular gland
Sublingual gland
Saliva
Innate immunity
Periodontitis
Glycoproteins
title_short Differential expression of salivary glycoproteins in aggressive and chronic periodontitis
title_full Differential expression of salivary glycoproteins in aggressive and chronic periodontitis
title_fullStr Differential expression of salivary glycoproteins in aggressive and chronic periodontitis
title_full_unstemmed Differential expression of salivary glycoproteins in aggressive and chronic periodontitis
title_sort Differential expression of salivary glycoproteins in aggressive and chronic periodontitis
author Rocha, Daniela de Morais
author_facet Rocha, Daniela de Morais
Zenóbio, Elton Gonçalves
Van Dyke, Thomas
Silva, Karine Simões
Costa, Fernando Oliveira
Soares, Rodrigo Villamarim
author_role author
author2 Zenóbio, Elton Gonçalves
Van Dyke, Thomas
Silva, Karine Simões
Costa, Fernando Oliveira
Soares, Rodrigo Villamarim
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Rocha, Daniela de Morais
Zenóbio, Elton Gonçalves
Van Dyke, Thomas
Silva, Karine Simões
Costa, Fernando Oliveira
Soares, Rodrigo Villamarim
dc.subject.por.fl_str_mv Submandibular gland
Sublingual gland
Saliva
Innate immunity
Periodontitis
Glycoproteins
topic Submandibular gland
Sublingual gland
Saliva
Innate immunity
Periodontitis
Glycoproteins
description OBJECTIVES:The aim of this study was to compare the pattern of secretion and the expression of mucin glycoprotein-2 (MG2) and lactoferrin in individuals with or without periodontitis. MATERIAL AND METHODS: Five individuals with aggressive periodontitis (APG), 5 with generalized chronic periodontitis (CPG) and 5 without periodontitis (CG) were enrolled after informed consent. Non-stimulated and stimulated submandibular and sublingual saliva was collected and samples analyzed by Western blot probed with specific antibodies. RESULTS: Stimulated and non-stimulated salivary flow rates did not differ among groups. Western blot analysis revealed that stimulation led to: an increase in MG2 expression in all groups, and to lactoferrin expression in APG and CPG. In non-stimulated saliva, CG exhibited the highest expression of both glycoproteins. In stimulated saliva, CG exhibited the highest expression of MG2, whereas APG the highest of lactoferrin. CONCLUSIONS: The pattern of secretion of MG2 and lactoferrin in health and disease is complex. Although the present study analyzed samples from a limited number of participants, the reduced expression of MG2 and lactoferrin in APG and CPG under non-stimulated condition, the predominant circumstance of salivary secretion during the day, suggests that these salivary constituents may play a role in the etiopathogenesis of these diseases.
publishDate 2012
dc.date.none.fl_str_mv 2012-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/46217
10.1590/S1678-77572012000200010
url https://www.revistas.usp.br/jaos/article/view/46217
identifier_str_mv 10.1590/S1678-77572012000200010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/46217/49839
dc.rights.driver.fl_str_mv Copyright (c) 2012 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2012 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 20 No. 2 (2012); 180-185
Journal of Applied Oral Science; Vol. 20 Núm. 2 (2012); 180-185
Journal of Applied Oral Science; v. 20 n. 2 (2012); 180-185
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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