Radioisotopic evaluation of bone repair after experimental surgical trauma
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572004000100014 |
Resumo: | BACKGROUND: Scientific approach of the bone reaction after surgical procedures provides valuable information on methods and techniques. The purpose of this study was to follow this process using a radioisotope marker of bone remodelling. MATERIAL AND METHODS: Two bone cavities were created (one for every tibia) in adult Wistar male rats using a 0.5 mm spherical burr; left tibial cavities were filled with bovine freeze-dried bone; the right ones were left unfilled for control. Scintigrams were done with sodium methylene diphosphonate (MDP) labelled with radioactive pertechnetate (99mTcO4-) to evaluate the inflammatory response and the local osteoblastic activity. The evolution of bone repair was additionally evaluated by light microscopy. RESULTS: Our results have shown that the highest bone activity was recorded between the 7th and the 14th day after surgery. The morphological analysis confirmed the results obtained with radioisotope analysis and did not reveal significant differences regarding the evolution of bone repair between the filled and the unfilled defects. CONCLUSION: We confirmed that 99mTc -MDP is a valuable tool to study bone repair, as it was able to show subtle alterations of bone activity even in lesions as small as those created herein (0.5 mm wide, 0.5 mm deep). |
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Journal of applied oral science (Online) |
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Radioisotopic evaluation of bone repair after experimental surgical traumaBone repairScintigraphyLight microscopyFreeze-dried boneSurgical traumaRatBACKGROUND: Scientific approach of the bone reaction after surgical procedures provides valuable information on methods and techniques. The purpose of this study was to follow this process using a radioisotope marker of bone remodelling. MATERIAL AND METHODS: Two bone cavities were created (one for every tibia) in adult Wistar male rats using a 0.5 mm spherical burr; left tibial cavities were filled with bovine freeze-dried bone; the right ones were left unfilled for control. Scintigrams were done with sodium methylene diphosphonate (MDP) labelled with radioactive pertechnetate (99mTcO4-) to evaluate the inflammatory response and the local osteoblastic activity. The evolution of bone repair was additionally evaluated by light microscopy. RESULTS: Our results have shown that the highest bone activity was recorded between the 7th and the 14th day after surgery. The morphological analysis confirmed the results obtained with radioisotope analysis and did not reveal significant differences regarding the evolution of bone repair between the filled and the unfilled defects. CONCLUSION: We confirmed that 99mTc -MDP is a valuable tool to study bone repair, as it was able to show subtle alterations of bone activity even in lesions as small as those created herein (0.5 mm wide, 0.5 mm deep).Faculdade De Odontologia De Bauru - USP2004-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572004000100014Journal of Applied Oral Science v.12 n.1 2004reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S1678-77572004000100014info:eu-repo/semantics/openAccessBreithaupt-Faloppa,Ana CristinaLara,Pedro FernandesSantos,Marinilce Fagundes dosOliveira-Filho,Ricardo MartinsCrivello Junior,Oswaldoeng2004-06-30T00:00:00Zoai:scielo:S1678-77572004000100014Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2004-06-30T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Radioisotopic evaluation of bone repair after experimental surgical trauma |
title |
Radioisotopic evaluation of bone repair after experimental surgical trauma |
spellingShingle |
Radioisotopic evaluation of bone repair after experimental surgical trauma Breithaupt-Faloppa,Ana Cristina Bone repair Scintigraphy Light microscopy Freeze-dried bone Surgical trauma Rat |
title_short |
Radioisotopic evaluation of bone repair after experimental surgical trauma |
title_full |
Radioisotopic evaluation of bone repair after experimental surgical trauma |
title_fullStr |
Radioisotopic evaluation of bone repair after experimental surgical trauma |
title_full_unstemmed |
Radioisotopic evaluation of bone repair after experimental surgical trauma |
title_sort |
Radioisotopic evaluation of bone repair after experimental surgical trauma |
author |
Breithaupt-Faloppa,Ana Cristina |
author_facet |
Breithaupt-Faloppa,Ana Cristina Lara,Pedro Fernandes Santos,Marinilce Fagundes dos Oliveira-Filho,Ricardo Martins Crivello Junior,Oswaldo |
author_role |
author |
author2 |
Lara,Pedro Fernandes Santos,Marinilce Fagundes dos Oliveira-Filho,Ricardo Martins Crivello Junior,Oswaldo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Breithaupt-Faloppa,Ana Cristina Lara,Pedro Fernandes Santos,Marinilce Fagundes dos Oliveira-Filho,Ricardo Martins Crivello Junior,Oswaldo |
dc.subject.por.fl_str_mv |
Bone repair Scintigraphy Light microscopy Freeze-dried bone Surgical trauma Rat |
topic |
Bone repair Scintigraphy Light microscopy Freeze-dried bone Surgical trauma Rat |
description |
BACKGROUND: Scientific approach of the bone reaction after surgical procedures provides valuable information on methods and techniques. The purpose of this study was to follow this process using a radioisotope marker of bone remodelling. MATERIAL AND METHODS: Two bone cavities were created (one for every tibia) in adult Wistar male rats using a 0.5 mm spherical burr; left tibial cavities were filled with bovine freeze-dried bone; the right ones were left unfilled for control. Scintigrams were done with sodium methylene diphosphonate (MDP) labelled with radioactive pertechnetate (99mTcO4-) to evaluate the inflammatory response and the local osteoblastic activity. The evolution of bone repair was additionally evaluated by light microscopy. RESULTS: Our results have shown that the highest bone activity was recorded between the 7th and the 14th day after surgery. The morphological analysis confirmed the results obtained with radioisotope analysis and did not reveal significant differences regarding the evolution of bone repair between the filled and the unfilled defects. CONCLUSION: We confirmed that 99mTc -MDP is a valuable tool to study bone repair, as it was able to show subtle alterations of bone activity even in lesions as small as those created herein (0.5 mm wide, 0.5 mm deep). |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572004000100014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572004000100014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1678-77572004000100014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science v.12 n.1 2004 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1748936433478926336 |