The effect of ellagic acid on the repair process of periodontal defects related to experimental periodontitis in rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/191379 |
Resumo: | Objective: This study aims to evaluate the effect of ellagic acid (EA) by measuring the levels of alveolar bone resorption and inflammatory and oxidative stress markers in the periodontal tissues and serum on the periodontal repair process related to experimental periodontitis in rats. Methodology: Forty Wistar rats were divided into four study groups as follows: Group 1=healthy control (n=10); Group 2=EA control (15 mg/kg)(n=10); Group 3=periodontitis (n=10); Group 4=periodontitis+EA (15 mg/kg) (n=10). The periodontitis model was established by ligating bilateral mandibular first molars for 14 days. Then, rats were given normal saline or EA for another 14 days by gavage administration. Serum and gingiva myeloperoxidase (MPO) activity, 8-hydroxydeoxyguanosine(8-OHdG), and glutathione (GSH) levels were analyzed by ELISA. İmmunohistochemical analysis was used to detect Interleukin (IL)-6, IL-10, and tumor necrosis factor-alpha (TNF-α) immunoreactivities in the periodontal tissues. Alveolar bone loss (ABL) and attachment loss (AL) was evaluated by histomorphometry analysis. Results: ABL and AL were statistically higher in group 3 than in groups 1, 2 and 4 and in group 4 than in groups 1 and 2 (p<0.05). MPO activities in gingival tissue and serum were significantly increased in group 3 compared to groups 1 and 2 (p<0.05). Significantly higher serum GSH levels, lower gingiva, and serum 8-OHdG levels, and MPO activity were observed in group 4 compared to group 3 (p<0.05). Rats with periodontitis (group 3) expressed significantly higher immunoreactivities of IL-6 and TNF-α and lower IL-10 immunoreactivity compared to those other groups (p<0.05). IL-6 and TNF-α immunoreactivities significantly decreased and IL-10 immunoreactivity increased in group 4 after the use of EA compared to group 3 (p<0.001). Conclusions: Our findings showed that EA provides significant improvements on gingival oxidative stress and inflammatory markers and alveolar bone resorption in the repair process associated with experimental periodontitis. Therefore, EA may have a therapeutic potential on periodontitis. |
id |
USP-17_7375c3c1aa0b9bd5fc68eb352df96ca5 |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/191379 |
network_acronym_str |
USP-17 |
network_name_str |
Journal of applied oral science (Online) |
repository_id_str |
|
spelling |
The effect of ellagic acid on the repair process of periodontal defects related to experimental periodontitis in ratsEllagic acidPeriodontitisPeriodontal repairCytokinesAntioxidantAlveolar bone defectObjective: This study aims to evaluate the effect of ellagic acid (EA) by measuring the levels of alveolar bone resorption and inflammatory and oxidative stress markers in the periodontal tissues and serum on the periodontal repair process related to experimental periodontitis in rats. Methodology: Forty Wistar rats were divided into four study groups as follows: Group 1=healthy control (n=10); Group 2=EA control (15 mg/kg)(n=10); Group 3=periodontitis (n=10); Group 4=periodontitis+EA (15 mg/kg) (n=10). The periodontitis model was established by ligating bilateral mandibular first molars for 14 days. Then, rats were given normal saline or EA for another 14 days by gavage administration. Serum and gingiva myeloperoxidase (MPO) activity, 8-hydroxydeoxyguanosine(8-OHdG), and glutathione (GSH) levels were analyzed by ELISA. İmmunohistochemical analysis was used to detect Interleukin (IL)-6, IL-10, and tumor necrosis factor-alpha (TNF-α) immunoreactivities in the periodontal tissues. Alveolar bone loss (ABL) and attachment loss (AL) was evaluated by histomorphometry analysis. Results: ABL and AL were statistically higher in group 3 than in groups 1, 2 and 4 and in group 4 than in groups 1 and 2 (p<0.05). MPO activities in gingival tissue and serum were significantly increased in group 3 compared to groups 1 and 2 (p<0.05). Significantly higher serum GSH levels, lower gingiva, and serum 8-OHdG levels, and MPO activity were observed in group 4 compared to group 3 (p<0.05). Rats with periodontitis (group 3) expressed significantly higher immunoreactivities of IL-6 and TNF-α and lower IL-10 immunoreactivity compared to those other groups (p<0.05). IL-6 and TNF-α immunoreactivities significantly decreased and IL-10 immunoreactivity increased in group 4 after the use of EA compared to group 3 (p<0.001). Conclusions: Our findings showed that EA provides significant improvements on gingival oxidative stress and inflammatory markers and alveolar bone resorption in the repair process associated with experimental periodontitis. Therefore, EA may have a therapeutic potential on periodontitis.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-10-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/19137910.1590/1678-7757-2021-0160 Journal of Applied Oral Science; Vol. 29 (2021); e20210160Journal of Applied Oral Science; Vol. 29 (2021); e20210160Journal of Applied Oral Science; v. 29 (2021); e202101601678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/191379/176425Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessÖngöz Dede, FigenDoğan, Şeyma Bozkurt Balli, UmutDurmuşlar, Mustafa Cenk Avci, BahattinGülle, KanatFerah, Meryem Akpolat 2021-10-13T13:37:35Zoai:revistas.usp.br:article/191379Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-10-13T13:37:35Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
The effect of ellagic acid on the repair process of periodontal defects related to experimental periodontitis in rats |
title |
The effect of ellagic acid on the repair process of periodontal defects related to experimental periodontitis in rats |
spellingShingle |
The effect of ellagic acid on the repair process of periodontal defects related to experimental periodontitis in rats Öngöz Dede, Figen Ellagic acid Periodontitis Periodontal repair Cytokines Antioxidant Alveolar bone defect |
title_short |
The effect of ellagic acid on the repair process of periodontal defects related to experimental periodontitis in rats |
title_full |
The effect of ellagic acid on the repair process of periodontal defects related to experimental periodontitis in rats |
title_fullStr |
The effect of ellagic acid on the repair process of periodontal defects related to experimental periodontitis in rats |
title_full_unstemmed |
The effect of ellagic acid on the repair process of periodontal defects related to experimental periodontitis in rats |
title_sort |
The effect of ellagic acid on the repair process of periodontal defects related to experimental periodontitis in rats |
author |
Öngöz Dede, Figen |
author_facet |
Öngöz Dede, Figen Doğan, Şeyma Bozkurt Balli, Umut Durmuşlar, Mustafa Cenk Avci, Bahattin Gülle, Kanat Ferah, Meryem Akpolat |
author_role |
author |
author2 |
Doğan, Şeyma Bozkurt Balli, Umut Durmuşlar, Mustafa Cenk Avci, Bahattin Gülle, Kanat Ferah, Meryem Akpolat |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Öngöz Dede, Figen Doğan, Şeyma Bozkurt Balli, Umut Durmuşlar, Mustafa Cenk Avci, Bahattin Gülle, Kanat Ferah, Meryem Akpolat |
dc.subject.por.fl_str_mv |
Ellagic acid Periodontitis Periodontal repair Cytokines Antioxidant Alveolar bone defect |
topic |
Ellagic acid Periodontitis Periodontal repair Cytokines Antioxidant Alveolar bone defect |
description |
Objective: This study aims to evaluate the effect of ellagic acid (EA) by measuring the levels of alveolar bone resorption and inflammatory and oxidative stress markers in the periodontal tissues and serum on the periodontal repair process related to experimental periodontitis in rats. Methodology: Forty Wistar rats were divided into four study groups as follows: Group 1=healthy control (n=10); Group 2=EA control (15 mg/kg)(n=10); Group 3=periodontitis (n=10); Group 4=periodontitis+EA (15 mg/kg) (n=10). The periodontitis model was established by ligating bilateral mandibular first molars for 14 days. Then, rats were given normal saline or EA for another 14 days by gavage administration. Serum and gingiva myeloperoxidase (MPO) activity, 8-hydroxydeoxyguanosine(8-OHdG), and glutathione (GSH) levels were analyzed by ELISA. İmmunohistochemical analysis was used to detect Interleukin (IL)-6, IL-10, and tumor necrosis factor-alpha (TNF-α) immunoreactivities in the periodontal tissues. Alveolar bone loss (ABL) and attachment loss (AL) was evaluated by histomorphometry analysis. Results: ABL and AL were statistically higher in group 3 than in groups 1, 2 and 4 and in group 4 than in groups 1 and 2 (p<0.05). MPO activities in gingival tissue and serum were significantly increased in group 3 compared to groups 1 and 2 (p<0.05). Significantly higher serum GSH levels, lower gingiva, and serum 8-OHdG levels, and MPO activity were observed in group 4 compared to group 3 (p<0.05). Rats with periodontitis (group 3) expressed significantly higher immunoreactivities of IL-6 and TNF-α and lower IL-10 immunoreactivity compared to those other groups (p<0.05). IL-6 and TNF-α immunoreactivities significantly decreased and IL-10 immunoreactivity increased in group 4 after the use of EA compared to group 3 (p<0.001). Conclusions: Our findings showed that EA provides significant improvements on gingival oxidative stress and inflammatory markers and alveolar bone resorption in the repair process associated with experimental periodontitis. Therefore, EA may have a therapeutic potential on periodontitis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/191379 10.1590/1678-7757-2021-0160 |
url |
https://www.revistas.usp.br/jaos/article/view/191379 |
identifier_str_mv |
10.1590/1678-7757-2021-0160 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/191379/176425 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 29 (2021); e20210160 Journal of Applied Oral Science; Vol. 29 (2021); e20210160 Journal of Applied Oral Science; v. 29 (2021); e20210160 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221682499059712 |