Microbial contamination and disinfection methods of pacifiers

Detalhes bibliográficos
Autor(a) principal: NELSON-FILHO,Paulo
Data de Publicação: 2015
Outros Autores: LOUVAIN,Márcia Costa, MACARI,Soraia, LUCISANO,Marília Pacífico, SILVA,Raquel Assed Bezerra da, QUEIROZ,Alexandra Mussolino de, GATON-HERNÁNDEZ,Patrícia, SILVA,Léa Assed Bezerra da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000500523
Resumo: Objectives To evaluate the microbial contamination of pacifiers by Mutans Streptococci (MS) and the efficacy of different methods for their disinfection. Methods Twenty-eight children were assigned to a 4-stage changeover system with a 1-week interval. In each stage, children received a new pacifier and the parents were instructed to maintain their normal habits for 1 week. After this time, the pacifiers were subjected to the following 4 disinfection methods: spraying with 0.12% chlorhexidine solution, Brushtox® or sterile tap water, and immersion in boiling tap water for 15 minutes. Microbiological culture for MS and Scanning Electron Microscopy (SEM) were performed. The results were analyzed statistically by Friedman’s non-parametric test (a=0.05). Results The 0.12% chlorhexidine spray was statistically similar to the boiling water (p>0.05) and more effective than the Brushtox® spray and control (p<0.05). The analysis of SEM showed the formation of a cariogenic biofilm in all groups with positive culture. Conclusions Pacifiers become contaminated by MS after their use by children and should be disinfected routinely. Spraying with a 0.12% chlorhexidine solution and immersion in boiling water promoted better disinfection of the pacifiers compared with a commercial antiseptic toothbrush cleanser (Brushtox®).
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spelling Microbial contamination and disinfection methods of pacifiersPacifiersChlorhexidineStreptococcus mutans Objectives To evaluate the microbial contamination of pacifiers by Mutans Streptococci (MS) and the efficacy of different methods for their disinfection. Methods Twenty-eight children were assigned to a 4-stage changeover system with a 1-week interval. In each stage, children received a new pacifier and the parents were instructed to maintain their normal habits for 1 week. After this time, the pacifiers were subjected to the following 4 disinfection methods: spraying with 0.12% chlorhexidine solution, Brushtox® or sterile tap water, and immersion in boiling tap water for 15 minutes. Microbiological culture for MS and Scanning Electron Microscopy (SEM) were performed. The results were analyzed statistically by Friedman’s non-parametric test (a=0.05). Results The 0.12% chlorhexidine spray was statistically similar to the boiling water (p>0.05) and more effective than the Brushtox® spray and control (p<0.05). The analysis of SEM showed the formation of a cariogenic biofilm in all groups with positive culture. Conclusions Pacifiers become contaminated by MS after their use by children and should be disinfected routinely. Spraying with a 0.12% chlorhexidine solution and immersion in boiling water promoted better disinfection of the pacifiers compared with a commercial antiseptic toothbrush cleanser (Brushtox®).Faculdade De Odontologia De Bauru - USP2015-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000500523Journal of Applied Oral Science v.23 n.5 2015reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-775720150244info:eu-repo/semantics/openAccessNELSON-FILHO,PauloLOUVAIN,Márcia CostaMACARI,SoraiaLUCISANO,Marília PacíficoSILVA,Raquel Assed Bezerra daQUEIROZ,Alexandra Mussolino deGATON-HERNÁNDEZ,PatríciaSILVA,Léa Assed Bezerra daeng2016-05-12T00:00:00Zoai:scielo:S1678-77572015000500523Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2016-05-12T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Microbial contamination and disinfection methods of pacifiers
title Microbial contamination and disinfection methods of pacifiers
spellingShingle Microbial contamination and disinfection methods of pacifiers
NELSON-FILHO,Paulo
Pacifiers
Chlorhexidine
Streptococcus mutans
title_short Microbial contamination and disinfection methods of pacifiers
title_full Microbial contamination and disinfection methods of pacifiers
title_fullStr Microbial contamination and disinfection methods of pacifiers
title_full_unstemmed Microbial contamination and disinfection methods of pacifiers
title_sort Microbial contamination and disinfection methods of pacifiers
author NELSON-FILHO,Paulo
author_facet NELSON-FILHO,Paulo
LOUVAIN,Márcia Costa
MACARI,Soraia
LUCISANO,Marília Pacífico
SILVA,Raquel Assed Bezerra da
QUEIROZ,Alexandra Mussolino de
GATON-HERNÁNDEZ,Patrícia
SILVA,Léa Assed Bezerra da
author_role author
author2 LOUVAIN,Márcia Costa
MACARI,Soraia
LUCISANO,Marília Pacífico
SILVA,Raquel Assed Bezerra da
QUEIROZ,Alexandra Mussolino de
GATON-HERNÁNDEZ,Patrícia
SILVA,Léa Assed Bezerra da
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv NELSON-FILHO,Paulo
LOUVAIN,Márcia Costa
MACARI,Soraia
LUCISANO,Marília Pacífico
SILVA,Raquel Assed Bezerra da
QUEIROZ,Alexandra Mussolino de
GATON-HERNÁNDEZ,Patrícia
SILVA,Léa Assed Bezerra da
dc.subject.por.fl_str_mv Pacifiers
Chlorhexidine
Streptococcus mutans
topic Pacifiers
Chlorhexidine
Streptococcus mutans
description Objectives To evaluate the microbial contamination of pacifiers by Mutans Streptococci (MS) and the efficacy of different methods for their disinfection. Methods Twenty-eight children were assigned to a 4-stage changeover system with a 1-week interval. In each stage, children received a new pacifier and the parents were instructed to maintain their normal habits for 1 week. After this time, the pacifiers were subjected to the following 4 disinfection methods: spraying with 0.12% chlorhexidine solution, Brushtox® or sterile tap water, and immersion in boiling tap water for 15 minutes. Microbiological culture for MS and Scanning Electron Microscopy (SEM) were performed. The results were analyzed statistically by Friedman’s non-parametric test (a=0.05). Results The 0.12% chlorhexidine spray was statistically similar to the boiling water (p>0.05) and more effective than the Brushtox® spray and control (p<0.05). The analysis of SEM showed the formation of a cariogenic biofilm in all groups with positive culture. Conclusions Pacifiers become contaminated by MS after their use by children and should be disinfected routinely. Spraying with a 0.12% chlorhexidine solution and immersion in boiling water promoted better disinfection of the pacifiers compared with a commercial antiseptic toothbrush cleanser (Brushtox®).
publishDate 2015
dc.date.none.fl_str_mv 2015-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000500523
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000500523
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-775720150244
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
dc.source.none.fl_str_mv Journal of Applied Oral Science v.23 n.5 2015
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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