Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000400338 |
Resumo: | ABSTRACT The successful use of composite resins in Dentistry depends on physicochemical properties, but also on the biological compatibility of resins, because of the close association between pulp and dentin. Objective The aim of this study was to evaluate cytotoxicity and cytokine production induced by light-cured or non-light-cured methacrylate-based and silorane composite resins in RAW 264.7 macrophages. Material and Methods Cells were stimulated with the extracts from light-cured or non-light-cured composite resins. After incubation for 24 h, cytotoxicity was assessed with the lactate dehydrogenase (LDH) and methyl thiazolyl tetrazolium (MTT) assays, and total protein was quantified using the Lowry method. TNF-α detection was examined with an enzyme-linked immunosorbent assay (ELISA) conducted with cell supernatants after cell stimulation for 6, 12, and 24 h. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey’s post hoc test (α=0.05). Results KaloreTM and FiltekTM Silorane were cytotoxic with or without light curing (p<0.05) after 24 h of incubation. KaloreTM stimulated the early production of TNF-α in comparison with control (p<0.05), whereas FiltekTM Silorane did not affect TNF-α levels after 6 and 12 h (p>0.05). However, after 24 h FiltekTM Silorane inhibited the production of TNF-α (p<0.05). Conclusions KaloreTM and FiltekTM Silorane were cytotoxic regardless of light curing. The extract obtained from KaloreTM after 15 days of incubation stimulated the production of TNF-α, unlike that obtained from FiltekTM Silorane. |
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USP-17 |
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Journal of applied oral science (Online) |
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Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resinsDental materialsComposite resinsCytotoxicityCytokinesABSTRACT The successful use of composite resins in Dentistry depends on physicochemical properties, but also on the biological compatibility of resins, because of the close association between pulp and dentin. Objective The aim of this study was to evaluate cytotoxicity and cytokine production induced by light-cured or non-light-cured methacrylate-based and silorane composite resins in RAW 264.7 macrophages. Material and Methods Cells were stimulated with the extracts from light-cured or non-light-cured composite resins. After incubation for 24 h, cytotoxicity was assessed with the lactate dehydrogenase (LDH) and methyl thiazolyl tetrazolium (MTT) assays, and total protein was quantified using the Lowry method. TNF-α detection was examined with an enzyme-linked immunosorbent assay (ELISA) conducted with cell supernatants after cell stimulation for 6, 12, and 24 h. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey’s post hoc test (α=0.05). Results KaloreTM and FiltekTM Silorane were cytotoxic with or without light curing (p<0.05) after 24 h of incubation. KaloreTM stimulated the early production of TNF-α in comparison with control (p<0.05), whereas FiltekTM Silorane did not affect TNF-α levels after 6 and 12 h (p>0.05). However, after 24 h FiltekTM Silorane inhibited the production of TNF-α (p<0.05). Conclusions KaloreTM and FiltekTM Silorane were cytotoxic regardless of light curing. The extract obtained from KaloreTM after 15 days of incubation stimulated the production of TNF-α, unlike that obtained from FiltekTM Silorane.Faculdade De Odontologia De Bauru - USP2016-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000400338Journal of Applied Oral Science v.24 n.4 2016reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-775720150449info:eu-repo/semantics/openAccessLONGO,Daniele LuccaPAULA-SILVA,Francisco Wanderley GarciaFACCIOLI,Lucia HelenaGATÓN-HERNÁNDEZ,Patrícia MariaQUEIROZ,Alexandra Mussolino deSILVA,Léa Assed Bezerra daeng2016-08-19T00:00:00Zoai:scielo:S1678-77572016000400338Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2016-08-19T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins |
title |
Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins |
spellingShingle |
Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins LONGO,Daniele Lucca Dental materials Composite resins Cytotoxicity Cytokines |
title_short |
Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins |
title_full |
Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins |
title_fullStr |
Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins |
title_full_unstemmed |
Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins |
title_sort |
Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins |
author |
LONGO,Daniele Lucca |
author_facet |
LONGO,Daniele Lucca PAULA-SILVA,Francisco Wanderley Garcia FACCIOLI,Lucia Helena GATÓN-HERNÁNDEZ,Patrícia Maria QUEIROZ,Alexandra Mussolino de SILVA,Léa Assed Bezerra da |
author_role |
author |
author2 |
PAULA-SILVA,Francisco Wanderley Garcia FACCIOLI,Lucia Helena GATÓN-HERNÁNDEZ,Patrícia Maria QUEIROZ,Alexandra Mussolino de SILVA,Léa Assed Bezerra da |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
LONGO,Daniele Lucca PAULA-SILVA,Francisco Wanderley Garcia FACCIOLI,Lucia Helena GATÓN-HERNÁNDEZ,Patrícia Maria QUEIROZ,Alexandra Mussolino de SILVA,Léa Assed Bezerra da |
dc.subject.por.fl_str_mv |
Dental materials Composite resins Cytotoxicity Cytokines |
topic |
Dental materials Composite resins Cytotoxicity Cytokines |
description |
ABSTRACT The successful use of composite resins in Dentistry depends on physicochemical properties, but also on the biological compatibility of resins, because of the close association between pulp and dentin. Objective The aim of this study was to evaluate cytotoxicity and cytokine production induced by light-cured or non-light-cured methacrylate-based and silorane composite resins in RAW 264.7 macrophages. Material and Methods Cells were stimulated with the extracts from light-cured or non-light-cured composite resins. After incubation for 24 h, cytotoxicity was assessed with the lactate dehydrogenase (LDH) and methyl thiazolyl tetrazolium (MTT) assays, and total protein was quantified using the Lowry method. TNF-α detection was examined with an enzyme-linked immunosorbent assay (ELISA) conducted with cell supernatants after cell stimulation for 6, 12, and 24 h. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey’s post hoc test (α=0.05). Results KaloreTM and FiltekTM Silorane were cytotoxic with or without light curing (p<0.05) after 24 h of incubation. KaloreTM stimulated the early production of TNF-α in comparison with control (p<0.05), whereas FiltekTM Silorane did not affect TNF-α levels after 6 and 12 h (p>0.05). However, after 24 h FiltekTM Silorane inhibited the production of TNF-α (p<0.05). Conclusions KaloreTM and FiltekTM Silorane were cytotoxic regardless of light curing. The extract obtained from KaloreTM after 15 days of incubation stimulated the production of TNF-α, unlike that obtained from FiltekTM Silorane. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000400338 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000400338 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-775720150449 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science v.24 n.4 2016 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1748936439005970432 |