Effect of ultrasound streaming on the disinfection of flattened root canals prepared by rotary and reciprocating systems
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/140015 |
Resumo: | New technical and scientific developments have been advocated to promote the success of the endodontic treatment. In addition to rotary and reciprocating systems, irrigating solution agitation has been suggested and passive ultrasonic irrigation (PUI) is the most used. Objective: To evaluate, in vitro, the effect of ultrasound streaming (US) in the disinfection of flattened root canal systems prepared by the ProTaper, BioRaCe and Reciproc systems, utilizing the microbiological culture. Methodology: Extracted human mandibular incisors (n=84) were used. Suspensions of Enterococcus faecalis (ATCC 29212) were standardized and inserted along with the teeth immersed in brain-heart infusion (BHI) broth. The contamination was made following a protocol during 5 days. The teeth were randomly divided into six groups: G1, ProTaper Universal; G2, ProTaper Universal with US; G3, BioRaCe; G4, BioRaCe with US; G5, Reciproc; and G6, Reciproc with US. Irrigation was performed with saline solution. After biomechanical preparation, microbiological samples were performed with sterilized paper points, which were diluted and spread on BHI agar; after 48 h, the colony forming units (CFU/mL) were counted for each sample. Results: Groups using ultrasonic agitation presented a greater antibacterial effect than the other ones, even using saline solution as irrigant. The ProTaper Universal system showed the best antibacterial activity of the tested systems (median of 0 CFU/mL with and without surfactant or ultrasonic activation [PUI]). Even with PUI, Reciproc (median of 2.5 CFU/mL with PUI and 5 without it) could not reduce as many colonies as ProTaper Universal without US. The BioRaCe system had greater bacterial reduction when using US (median of 0 CFU/mL with PUI and 30 without it). Conclusions: US promoted greater reduction in the number of bacteria in the flattened root canals prepared with nickel-titanium mechanized systems. Regarding the instruments used, the ProTaper Universal system was the most effective in reducing the bacterial number. |
id |
USP-17_a1cbd32b687c931e43233a1514b6d454 |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/140015 |
network_acronym_str |
USP-17 |
network_name_str |
Journal of applied oral science (Online) |
repository_id_str |
|
spelling |
Effect of ultrasound streaming on the disinfection of flattened root canals prepared by rotary and reciprocating systemsUltrasoundEnterococcus faecalisInstrumentationIrrigation New technical and scientific developments have been advocated to promote the success of the endodontic treatment. In addition to rotary and reciprocating systems, irrigating solution agitation has been suggested and passive ultrasonic irrigation (PUI) is the most used. Objective: To evaluate, in vitro, the effect of ultrasound streaming (US) in the disinfection of flattened root canal systems prepared by the ProTaper, BioRaCe and Reciproc systems, utilizing the microbiological culture. Methodology: Extracted human mandibular incisors (n=84) were used. Suspensions of Enterococcus faecalis (ATCC 29212) were standardized and inserted along with the teeth immersed in brain-heart infusion (BHI) broth. The contamination was made following a protocol during 5 days. The teeth were randomly divided into six groups: G1, ProTaper Universal; G2, ProTaper Universal with US; G3, BioRaCe; G4, BioRaCe with US; G5, Reciproc; and G6, Reciproc with US. Irrigation was performed with saline solution. After biomechanical preparation, microbiological samples were performed with sterilized paper points, which were diluted and spread on BHI agar; after 48 h, the colony forming units (CFU/mL) were counted for each sample. Results: Groups using ultrasonic agitation presented a greater antibacterial effect than the other ones, even using saline solution as irrigant. The ProTaper Universal system showed the best antibacterial activity of the tested systems (median of 0 CFU/mL with and without surfactant or ultrasonic activation [PUI]). Even with PUI, Reciproc (median of 2.5 CFU/mL with PUI and 5 without it) could not reduce as many colonies as ProTaper Universal without US. The BioRaCe system had greater bacterial reduction when using US (median of 0 CFU/mL with PUI and 30 without it). Conclusions: US promoted greater reduction in the number of bacteria in the flattened root canals prepared with nickel-titanium mechanized systems. Regarding the instruments used, the ProTaper Universal system was the most effective in reducing the bacterial number.Universidade de São Paulo. Faculdade de Odontologia de Bauru2017-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/14001510.1590/1678-7757-2016-0358Journal of Applied Oral Science; Vol. 25 No. 5 (2017); 477-482Journal of Applied Oral Science; Vol. 25 Núm. 5 (2017); 477-482Journal of Applied Oral Science; v. 25 n. 5 (2017); 477-4821678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/140015/135215Copyright (c) 2017 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessVasconcelos, Layla Reginna Silva Munhoz deMidena, Raquel ZaninMinotti, Paloma GagliardiPereira, Thais CristinaDuarte, Marco Antonio HungaroAndrade, Flaviana Bombarda de2017-10-25T11:51:34Zoai:revistas.usp.br:article/140015Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2017-10-25T11:51:34Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Effect of ultrasound streaming on the disinfection of flattened root canals prepared by rotary and reciprocating systems |
title |
Effect of ultrasound streaming on the disinfection of flattened root canals prepared by rotary and reciprocating systems |
spellingShingle |
Effect of ultrasound streaming on the disinfection of flattened root canals prepared by rotary and reciprocating systems Vasconcelos, Layla Reginna Silva Munhoz de Ultrasound Enterococcus faecalis Instrumentation Irrigation |
title_short |
Effect of ultrasound streaming on the disinfection of flattened root canals prepared by rotary and reciprocating systems |
title_full |
Effect of ultrasound streaming on the disinfection of flattened root canals prepared by rotary and reciprocating systems |
title_fullStr |
Effect of ultrasound streaming on the disinfection of flattened root canals prepared by rotary and reciprocating systems |
title_full_unstemmed |
Effect of ultrasound streaming on the disinfection of flattened root canals prepared by rotary and reciprocating systems |
title_sort |
Effect of ultrasound streaming on the disinfection of flattened root canals prepared by rotary and reciprocating systems |
author |
Vasconcelos, Layla Reginna Silva Munhoz de |
author_facet |
Vasconcelos, Layla Reginna Silva Munhoz de Midena, Raquel Zanin Minotti, Paloma Gagliardi Pereira, Thais Cristina Duarte, Marco Antonio Hungaro Andrade, Flaviana Bombarda de |
author_role |
author |
author2 |
Midena, Raquel Zanin Minotti, Paloma Gagliardi Pereira, Thais Cristina Duarte, Marco Antonio Hungaro Andrade, Flaviana Bombarda de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Vasconcelos, Layla Reginna Silva Munhoz de Midena, Raquel Zanin Minotti, Paloma Gagliardi Pereira, Thais Cristina Duarte, Marco Antonio Hungaro Andrade, Flaviana Bombarda de |
dc.subject.por.fl_str_mv |
Ultrasound Enterococcus faecalis Instrumentation Irrigation |
topic |
Ultrasound Enterococcus faecalis Instrumentation Irrigation |
description |
New technical and scientific developments have been advocated to promote the success of the endodontic treatment. In addition to rotary and reciprocating systems, irrigating solution agitation has been suggested and passive ultrasonic irrigation (PUI) is the most used. Objective: To evaluate, in vitro, the effect of ultrasound streaming (US) in the disinfection of flattened root canal systems prepared by the ProTaper, BioRaCe and Reciproc systems, utilizing the microbiological culture. Methodology: Extracted human mandibular incisors (n=84) were used. Suspensions of Enterococcus faecalis (ATCC 29212) were standardized and inserted along with the teeth immersed in brain-heart infusion (BHI) broth. The contamination was made following a protocol during 5 days. The teeth were randomly divided into six groups: G1, ProTaper Universal; G2, ProTaper Universal with US; G3, BioRaCe; G4, BioRaCe with US; G5, Reciproc; and G6, Reciproc with US. Irrigation was performed with saline solution. After biomechanical preparation, microbiological samples were performed with sterilized paper points, which were diluted and spread on BHI agar; after 48 h, the colony forming units (CFU/mL) were counted for each sample. Results: Groups using ultrasonic agitation presented a greater antibacterial effect than the other ones, even using saline solution as irrigant. The ProTaper Universal system showed the best antibacterial activity of the tested systems (median of 0 CFU/mL with and without surfactant or ultrasonic activation [PUI]). Even with PUI, Reciproc (median of 2.5 CFU/mL with PUI and 5 without it) could not reduce as many colonies as ProTaper Universal without US. The BioRaCe system had greater bacterial reduction when using US (median of 0 CFU/mL with PUI and 30 without it). Conclusions: US promoted greater reduction in the number of bacteria in the flattened root canals prepared with nickel-titanium mechanized systems. Regarding the instruments used, the ProTaper Universal system was the most effective in reducing the bacterial number. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/140015 10.1590/1678-7757-2016-0358 |
url |
https://www.revistas.usp.br/jaos/article/view/140015 |
identifier_str_mv |
10.1590/1678-7757-2016-0358 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/140015/135215 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 25 No. 5 (2017); 477-482 Journal of Applied Oral Science; Vol. 25 Núm. 5 (2017); 477-482 Journal of Applied Oral Science; v. 25 n. 5 (2017); 477-482 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221680421830656 |