Effect of two desensitizing agents on dentin permeability in vitro

Detalhes bibliográficos
Autor(a) principal: ISHIHATA, Hiroshi
Data de Publicação: 2017
Outros Autores: KANEHIRA, Masafumi, FINGER, Werner J., TAKAHASHI, Hidekazu, TOMITA, Makoto, SASAKI, Keiichi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/126732
Resumo: Objective The aim of this in vitro study was to investigate the effect of two desensitizing agents and water on hydraulic conductance in human dentin. Material and Methods GLUMA Desensitizer PowerGel (GLU) contains glutaraldehyde (GA) and 2-hydroxyethyl methacrylate (HEMA), and Teethmate Desensitizer (TD) is a powder comprising tetracalcium phosphate (TTCP) and dicalcium phosphate anhydrous (DCPA) that is mixed with water. Deionized water was used as a negative control (CTR). Thirty discs with a thickness of 1.2 mm were cut from the coronal dentin of the third molars and cleaned with 0.5 M EDTA (pH 7.4). After being mounted in a split-chamber device, the discs were pressurized with water at 1 kPa and 3 kPa in order to measure flow rates with a highly sensitive micro-flow sensor and to calculate hydraulic conductance as a baseline value (BL). Following the application of GLU, TD, and CTR (n=10), hydraulic conductance was remeasured with intermittent storage in water after 15 min, 1 d, 1 w, and 1 m. Reduction in permeability (PR%) was calculated from hydraulic conductance. Data were statistically analyzed using nonparametric methods (α
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spelling Effect of two desensitizing agents on dentin permeability in vitro Objective The aim of this in vitro study was to investigate the effect of two desensitizing agents and water on hydraulic conductance in human dentin. Material and Methods GLUMA Desensitizer PowerGel (GLU) contains glutaraldehyde (GA) and 2-hydroxyethyl methacrylate (HEMA), and Teethmate Desensitizer (TD) is a powder comprising tetracalcium phosphate (TTCP) and dicalcium phosphate anhydrous (DCPA) that is mixed with water. Deionized water was used as a negative control (CTR). Thirty discs with a thickness of 1.2 mm were cut from the coronal dentin of the third molars and cleaned with 0.5 M EDTA (pH 7.4). After being mounted in a split-chamber device, the discs were pressurized with water at 1 kPa and 3 kPa in order to measure flow rates with a highly sensitive micro-flow sensor and to calculate hydraulic conductance as a baseline value (BL). Following the application of GLU, TD, and CTR (n=10), hydraulic conductance was remeasured with intermittent storage in water after 15 min, 1 d, 1 w, and 1 m. Reduction in permeability (PR%) was calculated from hydraulic conductance. Data were statistically analyzed using nonparametric methods (αUniversidade de São Paulo. Faculdade de Odontologia de Bauru2017-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/12673210.1590/1678-77572016-0228Journal of Applied Oral Science; Vol. 25 No. 1 (2017); 34-41Journal of Applied Oral Science; Vol. 25 Núm. 1 (2017); 34-41Journal of Applied Oral Science; v. 25 n. 1 (2017); 34-411678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/126732/123712Copyright (c) 2017 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessISHIHATA, HiroshiKANEHIRA, MasafumiFINGER, Werner J.TAKAHASHI, HidekazuTOMITA, MakotoSASAKI, Keiichi2017-02-14T10:08:29Zoai:revistas.usp.br:article/126732Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2017-02-14T10:08:29Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Effect of two desensitizing agents on dentin permeability in vitro
title Effect of two desensitizing agents on dentin permeability in vitro
spellingShingle Effect of two desensitizing agents on dentin permeability in vitro
ISHIHATA, Hiroshi
title_short Effect of two desensitizing agents on dentin permeability in vitro
title_full Effect of two desensitizing agents on dentin permeability in vitro
title_fullStr Effect of two desensitizing agents on dentin permeability in vitro
title_full_unstemmed Effect of two desensitizing agents on dentin permeability in vitro
title_sort Effect of two desensitizing agents on dentin permeability in vitro
author ISHIHATA, Hiroshi
author_facet ISHIHATA, Hiroshi
KANEHIRA, Masafumi
FINGER, Werner J.
TAKAHASHI, Hidekazu
TOMITA, Makoto
SASAKI, Keiichi
author_role author
author2 KANEHIRA, Masafumi
FINGER, Werner J.
TAKAHASHI, Hidekazu
TOMITA, Makoto
SASAKI, Keiichi
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv ISHIHATA, Hiroshi
KANEHIRA, Masafumi
FINGER, Werner J.
TAKAHASHI, Hidekazu
TOMITA, Makoto
SASAKI, Keiichi
description Objective The aim of this in vitro study was to investigate the effect of two desensitizing agents and water on hydraulic conductance in human dentin. Material and Methods GLUMA Desensitizer PowerGel (GLU) contains glutaraldehyde (GA) and 2-hydroxyethyl methacrylate (HEMA), and Teethmate Desensitizer (TD) is a powder comprising tetracalcium phosphate (TTCP) and dicalcium phosphate anhydrous (DCPA) that is mixed with water. Deionized water was used as a negative control (CTR). Thirty discs with a thickness of 1.2 mm were cut from the coronal dentin of the third molars and cleaned with 0.5 M EDTA (pH 7.4). After being mounted in a split-chamber device, the discs were pressurized with water at 1 kPa and 3 kPa in order to measure flow rates with a highly sensitive micro-flow sensor and to calculate hydraulic conductance as a baseline value (BL). Following the application of GLU, TD, and CTR (n=10), hydraulic conductance was remeasured with intermittent storage in water after 15 min, 1 d, 1 w, and 1 m. Reduction in permeability (PR%) was calculated from hydraulic conductance. Data were statistically analyzed using nonparametric methods (α
publishDate 2017
dc.date.none.fl_str_mv 2017-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/126732
10.1590/1678-77572016-0228
url https://www.revistas.usp.br/jaos/article/view/126732
identifier_str_mv 10.1590/1678-77572016-0228
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/126732/123712
dc.rights.driver.fl_str_mv Copyright (c) 2017 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 25 No. 1 (2017); 34-41
Journal of Applied Oral Science; Vol. 25 Núm. 1 (2017); 34-41
Journal of Applied Oral Science; v. 25 n. 1 (2017); 34-41
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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