Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture

Detalhes bibliográficos
Autor(a) principal: Togashi,Adriane Yaeko
Data de Publicação: 2007
Outros Autores: Cirano,Fabiano Ribeiro, Marques,Márcia Martins, Pustiglioni,Francisco Emílio, Lima,Luiz Antonio Pugliesi Alves de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572007000500013
Resumo: The objective of the present study was to characterize bone cells grown in two culture media, and to determine the effective concentration of OP-1 on the growth of osteo-1 cells. Subcultured rat bone cells (osteo-1) were grown in alpha-modified Eagle's minimal essential medium (alpha-MEM) and Dulbecco's modified Eagle's medium (DMEM) and total protein content, alkaline phosphatase activity and the formation of mineralized nodules were evaluated after 7, 14 and 21 days. Cells were exposed to different concentrations of rhOP-1 for 1, 3, 5 and 7 days and compared with an untreated control. Osteo-1 cells presented a significant increase in alkaline phosphatase activity and calcium deposits were observed at 21 days. Cells treated with 10 and 20 ng/mL rhOP-1 for 24 h showed a significant increase in cell viability when compared to control. Osteo-1 cells cultured on DMEM demonstrated an osteoblastic phenotype as indicated by high alkaline phosphatase activity and the presence of calcified nodules. The results suggest that low concentrations of OP-1 may promote an osteogenic effect on osteo-1 cells.
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spelling Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subcultureOsteoblastsCell culture techniquesCell differentiationCalcification, physiologicalThe objective of the present study was to characterize bone cells grown in two culture media, and to determine the effective concentration of OP-1 on the growth of osteo-1 cells. Subcultured rat bone cells (osteo-1) were grown in alpha-modified Eagle's minimal essential medium (alpha-MEM) and Dulbecco's modified Eagle's medium (DMEM) and total protein content, alkaline phosphatase activity and the formation of mineralized nodules were evaluated after 7, 14 and 21 days. Cells were exposed to different concentrations of rhOP-1 for 1, 3, 5 and 7 days and compared with an untreated control. Osteo-1 cells presented a significant increase in alkaline phosphatase activity and calcium deposits were observed at 21 days. Cells treated with 10 and 20 ng/mL rhOP-1 for 24 h showed a significant increase in cell viability when compared to control. Osteo-1 cells cultured on DMEM demonstrated an osteoblastic phenotype as indicated by high alkaline phosphatase activity and the presence of calcified nodules. The results suggest that low concentrations of OP-1 may promote an osteogenic effect on osteo-1 cells.Faculdade De Odontologia De Bauru - USP2007-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572007000500013Journal of Applied Oral Science v.15 n.5 2007reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S1678-77572007000500013info:eu-repo/semantics/openAccessTogashi,Adriane YaekoCirano,Fabiano RibeiroMarques,Márcia MartinsPustiglioni,Francisco EmílioLima,Luiz Antonio Pugliesi Alves deeng2007-11-06T00:00:00Zoai:scielo:S1678-77572007000500013Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2007-11-06T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture
title Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture
spellingShingle Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture
Togashi,Adriane Yaeko
Osteoblasts
Cell culture techniques
Cell differentiation
Calcification, physiological
title_short Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture
title_full Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture
title_fullStr Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture
title_full_unstemmed Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture
title_sort Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture
author Togashi,Adriane Yaeko
author_facet Togashi,Adriane Yaeko
Cirano,Fabiano Ribeiro
Marques,Márcia Martins
Pustiglioni,Francisco Emílio
Lima,Luiz Antonio Pugliesi Alves de
author_role author
author2 Cirano,Fabiano Ribeiro
Marques,Márcia Martins
Pustiglioni,Francisco Emílio
Lima,Luiz Antonio Pugliesi Alves de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Togashi,Adriane Yaeko
Cirano,Fabiano Ribeiro
Marques,Márcia Martins
Pustiglioni,Francisco Emílio
Lima,Luiz Antonio Pugliesi Alves de
dc.subject.por.fl_str_mv Osteoblasts
Cell culture techniques
Cell differentiation
Calcification, physiological
topic Osteoblasts
Cell culture techniques
Cell differentiation
Calcification, physiological
description The objective of the present study was to characterize bone cells grown in two culture media, and to determine the effective concentration of OP-1 on the growth of osteo-1 cells. Subcultured rat bone cells (osteo-1) were grown in alpha-modified Eagle's minimal essential medium (alpha-MEM) and Dulbecco's modified Eagle's medium (DMEM) and total protein content, alkaline phosphatase activity and the formation of mineralized nodules were evaluated after 7, 14 and 21 days. Cells were exposed to different concentrations of rhOP-1 for 1, 3, 5 and 7 days and compared with an untreated control. Osteo-1 cells presented a significant increase in alkaline phosphatase activity and calcium deposits were observed at 21 days. Cells treated with 10 and 20 ng/mL rhOP-1 for 24 h showed a significant increase in cell viability when compared to control. Osteo-1 cells cultured on DMEM demonstrated an osteoblastic phenotype as indicated by high alkaline phosphatase activity and the presence of calcified nodules. The results suggest that low concentrations of OP-1 may promote an osteogenic effect on osteo-1 cells.
publishDate 2007
dc.date.none.fl_str_mv 2007-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572007000500013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572007000500013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1678-77572007000500013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
dc.source.none.fl_str_mv Journal of Applied Oral Science v.15 n.5 2007
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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