Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572007000500013 |
Resumo: | The objective of the present study was to characterize bone cells grown in two culture media, and to determine the effective concentration of OP-1 on the growth of osteo-1 cells. Subcultured rat bone cells (osteo-1) were grown in alpha-modified Eagle's minimal essential medium (alpha-MEM) and Dulbecco's modified Eagle's medium (DMEM) and total protein content, alkaline phosphatase activity and the formation of mineralized nodules were evaluated after 7, 14 and 21 days. Cells were exposed to different concentrations of rhOP-1 for 1, 3, 5 and 7 days and compared with an untreated control. Osteo-1 cells presented a significant increase in alkaline phosphatase activity and calcium deposits were observed at 21 days. Cells treated with 10 and 20 ng/mL rhOP-1 for 24 h showed a significant increase in cell viability when compared to control. Osteo-1 cells cultured on DMEM demonstrated an osteoblastic phenotype as indicated by high alkaline phosphatase activity and the presence of calcified nodules. The results suggest that low concentrations of OP-1 may promote an osteogenic effect on osteo-1 cells. |
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Journal of applied oral science (Online) |
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Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subcultureOsteoblastsCell culture techniquesCell differentiationCalcification, physiologicalThe objective of the present study was to characterize bone cells grown in two culture media, and to determine the effective concentration of OP-1 on the growth of osteo-1 cells. Subcultured rat bone cells (osteo-1) were grown in alpha-modified Eagle's minimal essential medium (alpha-MEM) and Dulbecco's modified Eagle's medium (DMEM) and total protein content, alkaline phosphatase activity and the formation of mineralized nodules were evaluated after 7, 14 and 21 days. Cells were exposed to different concentrations of rhOP-1 for 1, 3, 5 and 7 days and compared with an untreated control. Osteo-1 cells presented a significant increase in alkaline phosphatase activity and calcium deposits were observed at 21 days. Cells treated with 10 and 20 ng/mL rhOP-1 for 24 h showed a significant increase in cell viability when compared to control. Osteo-1 cells cultured on DMEM demonstrated an osteoblastic phenotype as indicated by high alkaline phosphatase activity and the presence of calcified nodules. The results suggest that low concentrations of OP-1 may promote an osteogenic effect on osteo-1 cells.Faculdade De Odontologia De Bauru - USP2007-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572007000500013Journal of Applied Oral Science v.15 n.5 2007reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S1678-77572007000500013info:eu-repo/semantics/openAccessTogashi,Adriane YaekoCirano,Fabiano RibeiroMarques,Márcia MartinsPustiglioni,Francisco EmílioLima,Luiz Antonio Pugliesi Alves deeng2007-11-06T00:00:00Zoai:scielo:S1678-77572007000500013Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2007-11-06T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture |
title |
Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture |
spellingShingle |
Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture Togashi,Adriane Yaeko Osteoblasts Cell culture techniques Cell differentiation Calcification, physiological |
title_short |
Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture |
title_full |
Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture |
title_fullStr |
Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture |
title_full_unstemmed |
Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture |
title_sort |
Characterization of bone cells obtained from the calvaria of neonatal rats (osteo-1) after serial subculture |
author |
Togashi,Adriane Yaeko |
author_facet |
Togashi,Adriane Yaeko Cirano,Fabiano Ribeiro Marques,Márcia Martins Pustiglioni,Francisco Emílio Lima,Luiz Antonio Pugliesi Alves de |
author_role |
author |
author2 |
Cirano,Fabiano Ribeiro Marques,Márcia Martins Pustiglioni,Francisco Emílio Lima,Luiz Antonio Pugliesi Alves de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Togashi,Adriane Yaeko Cirano,Fabiano Ribeiro Marques,Márcia Martins Pustiglioni,Francisco Emílio Lima,Luiz Antonio Pugliesi Alves de |
dc.subject.por.fl_str_mv |
Osteoblasts Cell culture techniques Cell differentiation Calcification, physiological |
topic |
Osteoblasts Cell culture techniques Cell differentiation Calcification, physiological |
description |
The objective of the present study was to characterize bone cells grown in two culture media, and to determine the effective concentration of OP-1 on the growth of osteo-1 cells. Subcultured rat bone cells (osteo-1) were grown in alpha-modified Eagle's minimal essential medium (alpha-MEM) and Dulbecco's modified Eagle's medium (DMEM) and total protein content, alkaline phosphatase activity and the formation of mineralized nodules were evaluated after 7, 14 and 21 days. Cells were exposed to different concentrations of rhOP-1 for 1, 3, 5 and 7 days and compared with an untreated control. Osteo-1 cells presented a significant increase in alkaline phosphatase activity and calcium deposits were observed at 21 days. Cells treated with 10 and 20 ng/mL rhOP-1 for 24 h showed a significant increase in cell viability when compared to control. Osteo-1 cells cultured on DMEM demonstrated an osteoblastic phenotype as indicated by high alkaline phosphatase activity and the presence of calcified nodules. The results suggest that low concentrations of OP-1 may promote an osteogenic effect on osteo-1 cells. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572007000500013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572007000500013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1678-77572007000500013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science v.15 n.5 2007 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1748936434776014848 |