Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems

Detalhes bibliográficos
Autor(a) principal: Gahyva, Sérgio Márcio Motta
Data de Publicação: 2005
Outros Autores: Siqueira Junior, José Freitas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
DOI: 10.1590/S1678-77572005000400014
Texto Completo: https://www.revistas.usp.br/jaos/article/view/3313
Resumo: Substances and materials used in endodontic therapy come into close contact with the periradicular tissues via apical foramen and foramina. Consequently, they should possess biocompatibility. There are currently few studies describing the genotoxic and mutagenic potentials of substances and materials used in endodontics. The purpose of this study was to evaluate the direct genotoxic and mutagenic properties of several substances and materials used in different phases of the endodontic treatment. For this intent, two prokaryotic test systems were used: the SOS chromotest and the Ames test. No metabolization with S9 was investigated, since only the direct effects of the substances and materials were surveyed. Most of the substances and materials tested presented mild to moderate cytotoxicity and genotoxicity as revealed by the SOS chromotest. Formocresol was the only tested substance to present severe genotoxicity to the tester bacterial strains. However, no substance or material tested showed direct mutagenicity as revealed by the Ames test.
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spelling Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems Genotoxicidade e mutagenicidade diretas de substâncias e materiais endodônticos avaliadas por dois testes procariotas Tratamento do canal radicularMedicação intracanalIrrigantes do canal radicularMateriais restauradores do canal radicularTestes de genotoxicidadeTestes de mutagenicidadeRoot canal therapyIntracanal medicationRoot canal irrigantsRoot canal filling materialsGenotoxycity testsMutagenicity tests Substances and materials used in endodontic therapy come into close contact with the periradicular tissues via apical foramen and foramina. Consequently, they should possess biocompatibility. There are currently few studies describing the genotoxic and mutagenic potentials of substances and materials used in endodontics. The purpose of this study was to evaluate the direct genotoxic and mutagenic properties of several substances and materials used in different phases of the endodontic treatment. For this intent, two prokaryotic test systems were used: the SOS chromotest and the Ames test. No metabolization with S9 was investigated, since only the direct effects of the substances and materials were surveyed. Most of the substances and materials tested presented mild to moderate cytotoxicity and genotoxicity as revealed by the SOS chromotest. Formocresol was the only tested substance to present severe genotoxicity to the tester bacterial strains. However, no substance or material tested showed direct mutagenicity as revealed by the Ames test. Substâncias e materiais utilizados na terapia endodôntica entram em íntimo contato com os tecidos perirradiculares via forame apical e foraminas e, em decorrência disto, deveriam idealmente possuir as seguintes características: biocompatibilidade e ausência de mutagenicidade. Existem poucos estudos avaliando o potencial genotóxico e mutagênico de substâncias e materiais utilizados em Endodontia. Este estudo avaliou os efeitos citotóxicos, genotóxicos e mutagênicos diretos de várias substâncias e materiais utilizados em diferentes etapas do tratamento endodôntico. Para isto, dois sistemas procarióticos foram usados: o SOS chromotest e o teste de Ames. Metabolização com fração S9 não foi realizada, uma vez que tencionou-se avaliar os efeitos diretos das substâncias e materiais. Os resultados demonstraram que algumas substâncias e materiais foram citotóxicos e/ou genotóxicos no SOS chromotest. Formocresol foi a única substância testada a apresentar efeitos genotóxicos severos sobre as cepas teste. Todavia, nenhum dos materiais ou substâncias avaliados apresentou mutagenicidade no teste de Ames. Universidade de São Paulo. Faculdade de Odontologia de Bauru2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/331310.1590/S1678-77572005000400014Journal of Applied Oral Science; Vol. 13 No. 4 (2005); 387-392 Journal of Applied Oral Science; Vol. 13 Núm. 4 (2005); 387-392 Journal of Applied Oral Science; v. 13 n. 4 (2005); 387-392 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3313/4003Copyright (c) 2005 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessGahyva, Sérgio Márcio MottaSiqueira Junior, José Freitas2012-04-27T11:51:58Zoai:revistas.usp.br:article/3313Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T11:51:58Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems
Genotoxicidade e mutagenicidade diretas de substâncias e materiais endodônticos avaliadas por dois testes procariotas
title Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems
spellingShingle Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems
Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems
Gahyva, Sérgio Márcio Motta
Tratamento do canal radicular
Medicação intracanal
Irrigantes do canal radicular
Materiais restauradores do canal radicular
Testes de genotoxicidade
Testes de mutagenicidade
Root canal therapy
Intracanal medication
Root canal irrigants
Root canal filling materials
Genotoxycity tests
Mutagenicity tests
Gahyva, Sérgio Márcio Motta
Tratamento do canal radicular
Medicação intracanal
Irrigantes do canal radicular
Materiais restauradores do canal radicular
Testes de genotoxicidade
Testes de mutagenicidade
Root canal therapy
Intracanal medication
Root canal irrigants
Root canal filling materials
Genotoxycity tests
Mutagenicity tests
title_short Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems
title_full Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems
title_fullStr Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems
Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems
title_full_unstemmed Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems
Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems
title_sort Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems
author Gahyva, Sérgio Márcio Motta
author_facet Gahyva, Sérgio Márcio Motta
Gahyva, Sérgio Márcio Motta
Siqueira Junior, José Freitas
Siqueira Junior, José Freitas
author_role author
author2 Siqueira Junior, José Freitas
author2_role author
dc.contributor.author.fl_str_mv Gahyva, Sérgio Márcio Motta
Siqueira Junior, José Freitas
dc.subject.por.fl_str_mv Tratamento do canal radicular
Medicação intracanal
Irrigantes do canal radicular
Materiais restauradores do canal radicular
Testes de genotoxicidade
Testes de mutagenicidade
Root canal therapy
Intracanal medication
Root canal irrigants
Root canal filling materials
Genotoxycity tests
Mutagenicity tests
topic Tratamento do canal radicular
Medicação intracanal
Irrigantes do canal radicular
Materiais restauradores do canal radicular
Testes de genotoxicidade
Testes de mutagenicidade
Root canal therapy
Intracanal medication
Root canal irrigants
Root canal filling materials
Genotoxycity tests
Mutagenicity tests
description Substances and materials used in endodontic therapy come into close contact with the periradicular tissues via apical foramen and foramina. Consequently, they should possess biocompatibility. There are currently few studies describing the genotoxic and mutagenic potentials of substances and materials used in endodontics. The purpose of this study was to evaluate the direct genotoxic and mutagenic properties of several substances and materials used in different phases of the endodontic treatment. For this intent, two prokaryotic test systems were used: the SOS chromotest and the Ames test. No metabolization with S9 was investigated, since only the direct effects of the substances and materials were surveyed. Most of the substances and materials tested presented mild to moderate cytotoxicity and genotoxicity as revealed by the SOS chromotest. Formocresol was the only tested substance to present severe genotoxicity to the tester bacterial strains. However, no substance or material tested showed direct mutagenicity as revealed by the Ames test.
publishDate 2005
dc.date.none.fl_str_mv 2005-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3313
10.1590/S1678-77572005000400014
url https://www.revistas.usp.br/jaos/article/view/3313
identifier_str_mv 10.1590/S1678-77572005000400014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3313/4003
dc.rights.driver.fl_str_mv Copyright (c) 2005 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2005 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 13 No. 4 (2005); 387-392
Journal of Applied Oral Science; Vol. 13 Núm. 4 (2005); 387-392
Journal of Applied Oral Science; v. 13 n. 4 (2005); 387-392
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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dc.identifier.doi.none.fl_str_mv 10.1590/S1678-77572005000400014

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