Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100430 |
Resumo: | Abstract Titanium tetrafluoride (TiF4) is known for interacting with enamel reducing demineralization. However, no information is available about its potential antimicrobial effect. Objectives This study evaluated the antimicrobial and anti-caries potential of TiF4 varnish compared to NaF varnish, chlorhexidine gel (positive control), placebo varnish and untreated (negative controls) using a dental microcosm biofilm model. Material and Methods A microcosm biofilm was produced on bovine enamel previously treated with the varnishes, using inoculum from human saliva mixed with McBain saliva, under 0.2% sucrose exposure, for 14 days. All experiments were performed in biological triplicate (n=4/group in each experiment). Factors evaluated were: bacterial viability (% dead and live bacteria); CFU counting (log10 CFU/mL); and enamel demineralization (transverse microradiography – TMR). Data were analysed using ANOVA/Tukey's test or Kruskal-Wallis/Dunn's test (p<0.05). Results Only chlorhexidine significantly increased the number of dead bacteria (68.8±13.1% dead bacteria) compared to untreated control (48.9±16.1% dead bacteria). No treatment reduced the CFU counting (total microorganism and total streptococci) compared to the negative controls. Only TiF4 was able to reduce enamel demineralization (ΔZ 1110.7±803.2 vol% μm) compared to both negative controls (untreated: ΔZ 4455.3±1176.4 vol% μm). Conclusions TiF4 varnish has no relevant antimicrobial effect. Nevertheless, TiF4 varnish was effective in reducing enamel demineralization under this model. |
id |
USP-17_c1c91c94835756e18b487cdae03cb455 |
---|---|
oai_identifier_str |
oai:scielo:S1678-77572018000100430 |
network_acronym_str |
USP-17 |
network_name_str |
Journal of applied oral science (Online) |
repository_id_str |
|
spelling |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamelDental biofilmEnamel cariesFluorideTitaniumVarnishAbstract Titanium tetrafluoride (TiF4) is known for interacting with enamel reducing demineralization. However, no information is available about its potential antimicrobial effect. Objectives This study evaluated the antimicrobial and anti-caries potential of TiF4 varnish compared to NaF varnish, chlorhexidine gel (positive control), placebo varnish and untreated (negative controls) using a dental microcosm biofilm model. Material and Methods A microcosm biofilm was produced on bovine enamel previously treated with the varnishes, using inoculum from human saliva mixed with McBain saliva, under 0.2% sucrose exposure, for 14 days. All experiments were performed in biological triplicate (n=4/group in each experiment). Factors evaluated were: bacterial viability (% dead and live bacteria); CFU counting (log10 CFU/mL); and enamel demineralization (transverse microradiography – TMR). Data were analysed using ANOVA/Tukey's test or Kruskal-Wallis/Dunn's test (p<0.05). Results Only chlorhexidine significantly increased the number of dead bacteria (68.8±13.1% dead bacteria) compared to untreated control (48.9±16.1% dead bacteria). No treatment reduced the CFU counting (total microorganism and total streptococci) compared to the negative controls. Only TiF4 was able to reduce enamel demineralization (ΔZ 1110.7±803.2 vol% μm) compared to both negative controls (untreated: ΔZ 4455.3±1176.4 vol% μm). Conclusions TiF4 varnish has no relevant antimicrobial effect. Nevertheless, TiF4 varnish was effective in reducing enamel demineralization under this model.Faculdade De Odontologia De Bauru - USP2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100430Journal of Applied Oral Science v.26 2018reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-7757-2017-0304info:eu-repo/semantics/openAccessSouza,Beatriz Martines deFernandes Neto,ConstantinoSalomão,Priscila Maria ArandaVasconcelos,Layla Reginna Silva Munhoz deAndrade,Flaviana Bombarda deMagalhães,Ana Carolinaeng2018-02-20T00:00:00Zoai:scielo:S1678-77572018000100430Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2018-02-20T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
title |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
spellingShingle |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel Souza,Beatriz Martines de Dental biofilm Enamel caries Fluoride Titanium Varnish |
title_short |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
title_full |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
title_fullStr |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
title_full_unstemmed |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
title_sort |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
author |
Souza,Beatriz Martines de |
author_facet |
Souza,Beatriz Martines de Fernandes Neto,Constantino Salomão,Priscila Maria Aranda Vasconcelos,Layla Reginna Silva Munhoz de Andrade,Flaviana Bombarda de Magalhães,Ana Carolina |
author_role |
author |
author2 |
Fernandes Neto,Constantino Salomão,Priscila Maria Aranda Vasconcelos,Layla Reginna Silva Munhoz de Andrade,Flaviana Bombarda de Magalhães,Ana Carolina |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Souza,Beatriz Martines de Fernandes Neto,Constantino Salomão,Priscila Maria Aranda Vasconcelos,Layla Reginna Silva Munhoz de Andrade,Flaviana Bombarda de Magalhães,Ana Carolina |
dc.subject.por.fl_str_mv |
Dental biofilm Enamel caries Fluoride Titanium Varnish |
topic |
Dental biofilm Enamel caries Fluoride Titanium Varnish |
description |
Abstract Titanium tetrafluoride (TiF4) is known for interacting with enamel reducing demineralization. However, no information is available about its potential antimicrobial effect. Objectives This study evaluated the antimicrobial and anti-caries potential of TiF4 varnish compared to NaF varnish, chlorhexidine gel (positive control), placebo varnish and untreated (negative controls) using a dental microcosm biofilm model. Material and Methods A microcosm biofilm was produced on bovine enamel previously treated with the varnishes, using inoculum from human saliva mixed with McBain saliva, under 0.2% sucrose exposure, for 14 days. All experiments were performed in biological triplicate (n=4/group in each experiment). Factors evaluated were: bacterial viability (% dead and live bacteria); CFU counting (log10 CFU/mL); and enamel demineralization (transverse microradiography – TMR). Data were analysed using ANOVA/Tukey's test or Kruskal-Wallis/Dunn's test (p<0.05). Results Only chlorhexidine significantly increased the number of dead bacteria (68.8±13.1% dead bacteria) compared to untreated control (48.9±16.1% dead bacteria). No treatment reduced the CFU counting (total microorganism and total streptococci) compared to the negative controls. Only TiF4 was able to reduce enamel demineralization (ΔZ 1110.7±803.2 vol% μm) compared to both negative controls (untreated: ΔZ 4455.3±1176.4 vol% μm). Conclusions TiF4 varnish has no relevant antimicrobial effect. Nevertheless, TiF4 varnish was effective in reducing enamel demineralization under this model. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100430 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100430 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-7757-2017-0304 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science v.26 2018 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1748936439569055744 |