Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/144552 |
Resumo: | Titanium tetrafluoride (TiF4) is known for interacting with enamel reducing demineralization. However, no information is available about its potential antimicrobial effect. Objectives This study evaluated the antimicrobial and anti-caries potential of TiF4 varnish compared to NaF varnish, chlorhexidine gel (positive control), placebo varnish and untreated (negative controls) using a dental microcosm biofilm model. Material and Methods A microcosm biofilm was produced on bovine enamel previously treated with the varnishes, using inoculum from human saliva mixed with McBain saliva, under 0.2% sucrose exposure, for 14 days. All experiments were performed in biological triplicate (n=4/group in each experiment). Factors evaluated were: bacterial viability (% dead and live bacteria); CFU counting (log10 CFU/mL); and enamel demineralization (transverse microradiography – TMR). Data were analysed using ANOVA/Tukey's test or Kruskal-Wallis/Dunn's test (p |
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Journal of applied oral science (Online) |
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Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamelDental biofilmEnamel cariesFluorideTitaniumVarnish Titanium tetrafluoride (TiF4) is known for interacting with enamel reducing demineralization. However, no information is available about its potential antimicrobial effect. Objectives This study evaluated the antimicrobial and anti-caries potential of TiF4 varnish compared to NaF varnish, chlorhexidine gel (positive control), placebo varnish and untreated (negative controls) using a dental microcosm biofilm model. Material and Methods A microcosm biofilm was produced on bovine enamel previously treated with the varnishes, using inoculum from human saliva mixed with McBain saliva, under 0.2% sucrose exposure, for 14 days. All experiments were performed in biological triplicate (n=4/group in each experiment). Factors evaluated were: bacterial viability (% dead and live bacteria); CFU counting (log10 CFU/mL); and enamel demineralization (transverse microradiography – TMR). Data were analysed using ANOVA/Tukey's test or Kruskal-Wallis/Dunn's test (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/14455210.1590/1678-7757-2017-0304Journal of Applied Oral Science; Vol. 26 (2018); e20170304Journal of Applied Oral Science; Vol. 26 (2018); e20170304Journal of Applied Oral Science; v. 26 (2018); e201703041678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/144552/138875Copyright (c) 2018 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessSouza, Beatriz Martines deFernandes Neto, ConstantinoSalomão, Priscila Maria ArandaVasconcelos, Layla Reginna Silva Munhoz deAndrade, Flaviana Bombarda deMagalhães, Ana Carolina2018-03-20T12:30:07Zoai:revistas.usp.br:article/144552Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2018-03-20T12:30:07Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
title |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
spellingShingle |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel Souza, Beatriz Martines de Dental biofilm Enamel caries Fluoride Titanium Varnish |
title_short |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
title_full |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
title_fullStr |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
title_full_unstemmed |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
title_sort |
Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel |
author |
Souza, Beatriz Martines de |
author_facet |
Souza, Beatriz Martines de Fernandes Neto, Constantino Salomão, Priscila Maria Aranda Vasconcelos, Layla Reginna Silva Munhoz de Andrade, Flaviana Bombarda de Magalhães, Ana Carolina |
author_role |
author |
author2 |
Fernandes Neto, Constantino Salomão, Priscila Maria Aranda Vasconcelos, Layla Reginna Silva Munhoz de Andrade, Flaviana Bombarda de Magalhães, Ana Carolina |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Souza, Beatriz Martines de Fernandes Neto, Constantino Salomão, Priscila Maria Aranda Vasconcelos, Layla Reginna Silva Munhoz de Andrade, Flaviana Bombarda de Magalhães, Ana Carolina |
dc.subject.por.fl_str_mv |
Dental biofilm Enamel caries Fluoride Titanium Varnish |
topic |
Dental biofilm Enamel caries Fluoride Titanium Varnish |
description |
Titanium tetrafluoride (TiF4) is known for interacting with enamel reducing demineralization. However, no information is available about its potential antimicrobial effect. Objectives This study evaluated the antimicrobial and anti-caries potential of TiF4 varnish compared to NaF varnish, chlorhexidine gel (positive control), placebo varnish and untreated (negative controls) using a dental microcosm biofilm model. Material and Methods A microcosm biofilm was produced on bovine enamel previously treated with the varnishes, using inoculum from human saliva mixed with McBain saliva, under 0.2% sucrose exposure, for 14 days. All experiments were performed in biological triplicate (n=4/group in each experiment). Factors evaluated were: bacterial viability (% dead and live bacteria); CFU counting (log10 CFU/mL); and enamel demineralization (transverse microradiography – TMR). Data were analysed using ANOVA/Tukey's test or Kruskal-Wallis/Dunn's test (p |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/144552 10.1590/1678-7757-2017-0304 |
url |
https://www.revistas.usp.br/jaos/article/view/144552 |
identifier_str_mv |
10.1590/1678-7757-2017-0304 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/144552/138875 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 26 (2018); e20170304 Journal of Applied Oral Science; Vol. 26 (2018); e20170304 Journal of Applied Oral Science; v. 26 (2018); e20170304 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221681103405056 |