Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway

Detalhes bibliográficos
Autor(a) principal: Jianru,YI
Data de Publicação: 2015
Outros Autores: MeiLe,LI, YANG,Yan, ZHENG,Wei, Yu,LI, ZHAO,Zhihe
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000600549
Resumo: ABSTRACT Objective This study aimed to investigate the potential role of CAMK II pathway in the compression-regulated OPG expression in periodontal ligament cells (PDLCs). Material and Methods The PDL tissue model was developed by 3-D culturing human PDLCs in a thin sheet of poly lactic-co-glycolic acid (PLGA) scaffolds, which was subjected to static compression of 25 g/cm2 for 3, 6 and 12 h, with or without treatment of KN-93. After that, the expression of OPG, RANKL and NFATC2 was investigated through real-time PCR and western blot analysis. Results After static compression, the NFATC2 and RANKL expression was significantly up-regulated, while partially suppressed by KN-93 for 6 and 12 h respectively. The OPG expression was significantly down-regulated by compression in 3 h, started to elevate in 6 h, and significantly up-regulated in 12 h. The up-regulation after 12 h was significantly suppressed by KN-93. Conclusions Long-term static compression increases OPG expression in PDLCs, at least partially, via the CAMK II pathway.
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spelling Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathwayOsteogenesisPeriodontal ligamentCalcium-calmodulin-dependent Protein kinasesMechanotransductionABSTRACT Objective This study aimed to investigate the potential role of CAMK II pathway in the compression-regulated OPG expression in periodontal ligament cells (PDLCs). Material and Methods The PDL tissue model was developed by 3-D culturing human PDLCs in a thin sheet of poly lactic-co-glycolic acid (PLGA) scaffolds, which was subjected to static compression of 25 g/cm2 for 3, 6 and 12 h, with or without treatment of KN-93. After that, the expression of OPG, RANKL and NFATC2 was investigated through real-time PCR and western blot analysis. Results After static compression, the NFATC2 and RANKL expression was significantly up-regulated, while partially suppressed by KN-93 for 6 and 12 h respectively. The OPG expression was significantly down-regulated by compression in 3 h, started to elevate in 6 h, and significantly up-regulated in 12 h. The up-regulation after 12 h was significantly suppressed by KN-93. Conclusions Long-term static compression increases OPG expression in PDLCs, at least partially, via the CAMK II pathway.Faculdade De Odontologia De Bauru - USP2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000600549Journal of Applied Oral Science v.23 n.6 2015reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-775720150156info:eu-repo/semantics/openAccessJianru,YIMeiLe,LIYANG,YanZHENG,WeiYu,LIZHAO,Zhiheeng2016-05-12T00:00:00Zoai:scielo:S1678-77572015000600549Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2016-05-12T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway
title Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway
spellingShingle Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway
Jianru,YI
Osteogenesis
Periodontal ligament
Calcium-calmodulin-dependent Protein kinases
Mechanotransduction
title_short Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway
title_full Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway
title_fullStr Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway
title_full_unstemmed Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway
title_sort Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway
author Jianru,YI
author_facet Jianru,YI
MeiLe,LI
YANG,Yan
ZHENG,Wei
Yu,LI
ZHAO,Zhihe
author_role author
author2 MeiLe,LI
YANG,Yan
ZHENG,Wei
Yu,LI
ZHAO,Zhihe
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Jianru,YI
MeiLe,LI
YANG,Yan
ZHENG,Wei
Yu,LI
ZHAO,Zhihe
dc.subject.por.fl_str_mv Osteogenesis
Periodontal ligament
Calcium-calmodulin-dependent Protein kinases
Mechanotransduction
topic Osteogenesis
Periodontal ligament
Calcium-calmodulin-dependent Protein kinases
Mechanotransduction
description ABSTRACT Objective This study aimed to investigate the potential role of CAMK II pathway in the compression-regulated OPG expression in periodontal ligament cells (PDLCs). Material and Methods The PDL tissue model was developed by 3-D culturing human PDLCs in a thin sheet of poly lactic-co-glycolic acid (PLGA) scaffolds, which was subjected to static compression of 25 g/cm2 for 3, 6 and 12 h, with or without treatment of KN-93. After that, the expression of OPG, RANKL and NFATC2 was investigated through real-time PCR and western blot analysis. Results After static compression, the NFATC2 and RANKL expression was significantly up-regulated, while partially suppressed by KN-93 for 6 and 12 h respectively. The OPG expression was significantly down-regulated by compression in 3 h, started to elevate in 6 h, and significantly up-regulated in 12 h. The up-regulation after 12 h was significantly suppressed by KN-93. Conclusions Long-term static compression increases OPG expression in PDLCs, at least partially, via the CAMK II pathway.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000600549
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000600549
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-775720150156
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
dc.source.none.fl_str_mv Journal of Applied Oral Science v.23 n.6 2015
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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