Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/110620 |
Resumo: | Objective This study aimed to investigate the potential role of CAMK II pathway in the compression-regulated OPG expression in periodontal ligament cells (PDLCs). Material and Methods The PDL tissue model was developed by 3-D culturing human PDLCs in a thin sheet of poly lactic-co-glycolic acid (PLGA) scaffolds, which was subjected to static compression of 25 g/cm2 for 3, 6 and 12 h, with or without treatment of KN-93. After that, the expression of OPG, RANKL and NFATC2 was investigated through real-time PCR and western blot analysis. Results After static compression, the NFATC2 and RANKL expression was significantly up-regulated, while partially suppressed by KN-93 for 6 and 12 h respectively. The OPG expression was significantly down-regulated by compression in 3 h, started to elevate in 6 h, and significantly up-regulated in 12 h. The up-regulation after 12 h was significantly suppressed by KN-93. Conclusions Long-term static compression increases OPG expression in PDLCs, at least partially, via the CAMK II pathway. |
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oai:revistas.usp.br:article/110620 |
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USP-17 |
network_name_str |
Journal of applied oral science (Online) |
repository_id_str |
|
spelling |
Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway Objective This study aimed to investigate the potential role of CAMK II pathway in the compression-regulated OPG expression in periodontal ligament cells (PDLCs). Material and Methods The PDL tissue model was developed by 3-D culturing human PDLCs in a thin sheet of poly lactic-co-glycolic acid (PLGA) scaffolds, which was subjected to static compression of 25 g/cm2 for 3, 6 and 12 h, with or without treatment of KN-93. After that, the expression of OPG, RANKL and NFATC2 was investigated through real-time PCR and western blot analysis. Results After static compression, the NFATC2 and RANKL expression was significantly up-regulated, while partially suppressed by KN-93 for 6 and 12 h respectively. The OPG expression was significantly down-regulated by compression in 3 h, started to elevate in 6 h, and significantly up-regulated in 12 h. The up-regulation after 12 h was significantly suppressed by KN-93. Conclusions Long-term static compression increases OPG expression in PDLCs, at least partially, via the CAMK II pathway. Universidade de São Paulo. Faculdade de Odontologia de Bauru2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/11062010.1590/1678-775720150156Journal of Applied Oral Science; Vol. 23 No. 6 (2015); 549-554Journal of Applied Oral Science; Vol. 23 Núm. 6 (2015); 549-554Journal of Applied Oral Science; v. 23 n. 6 (2015); 549-5541678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/110620/109094Copyright (c) 2015 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessJianru, YIMeiLe, LIYANG, YanZHENG, WeiYu, LIZHAO, Zhihe2016-02-01T17:03:34Zoai:revistas.usp.br:article/110620Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2016-02-01T17:03:34Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway |
title |
Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway |
spellingShingle |
Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway Jianru, YI |
title_short |
Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway |
title_full |
Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway |
title_fullStr |
Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway |
title_full_unstemmed |
Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway |
title_sort |
Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway |
author |
Jianru, YI |
author_facet |
Jianru, YI MeiLe, LI YANG, Yan ZHENG, Wei Yu, LI ZHAO, Zhihe |
author_role |
author |
author2 |
MeiLe, LI YANG, Yan ZHENG, Wei Yu, LI ZHAO, Zhihe |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Jianru, YI MeiLe, LI YANG, Yan ZHENG, Wei Yu, LI ZHAO, Zhihe |
description |
Objective This study aimed to investigate the potential role of CAMK II pathway in the compression-regulated OPG expression in periodontal ligament cells (PDLCs). Material and Methods The PDL tissue model was developed by 3-D culturing human PDLCs in a thin sheet of poly lactic-co-glycolic acid (PLGA) scaffolds, which was subjected to static compression of 25 g/cm2 for 3, 6 and 12 h, with or without treatment of KN-93. After that, the expression of OPG, RANKL and NFATC2 was investigated through real-time PCR and western blot analysis. Results After static compression, the NFATC2 and RANKL expression was significantly up-regulated, while partially suppressed by KN-93 for 6 and 12 h respectively. The OPG expression was significantly down-regulated by compression in 3 h, started to elevate in 6 h, and significantly up-regulated in 12 h. The up-regulation after 12 h was significantly suppressed by KN-93. Conclusions Long-term static compression increases OPG expression in PDLCs, at least partially, via the CAMK II pathway. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/110620 10.1590/1678-775720150156 |
url |
https://www.revistas.usp.br/jaos/article/view/110620 |
identifier_str_mv |
10.1590/1678-775720150156 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/110620/109094 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 23 No. 6 (2015); 549-554 Journal of Applied Oral Science; Vol. 23 Núm. 6 (2015); 549-554 Journal of Applied Oral Science; v. 23 n. 6 (2015); 549-554 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221678866792448 |