Transcriptional analysis of the human PAX9 promoter
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/3830 |
Resumo: | OBJECTIVES: PAX9 belongs to the Pax family of transcriptional factor genes. This gene is expressed in embryonic tissues such as somites, pharyngeal pouch endoderm, distal limb buds and neural crest-derived mesenchyme. Polymorphisms in the upstream promoter region of the human PAX9 have been associated with human non-syndromic tooth agenesis. In the present study, we verified the in vitro mRNA expression of this gene and the luciferase activity of two constructs containing promoter sequences of the PAX9 gene. MATERIAL AND METHODS: Embryonic tissues were obtained from digits, face, and midbrain/hindbrain regions. Fragments containing PAX9 promoter sequences were cloned into reporter plasmids and were transfected into the different cell cultures. mRNA were extracted from primary cell cultures. RESULTS: The semi-quantitative RT-PCR results showed that in vitro E13.5 limb bud and CNS cells express PAX9, but cells derived from the facial region do not. Moreover, the luciferase assay showed that protein activity of the constructed vector was weaker than pgl3 -basic alone. CONCLUSIONS: The present results suggest that the promoter sequences analyzed are not sufficient to drive PAX9 gene transcription. |
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oai:revistas.usp.br:article/3830 |
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Journal of applied oral science (Online) |
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Transcriptional analysis of the human PAX9 promoter PAX9 transcription factorGenetic promoter regionsAnodontia OBJECTIVES: PAX9 belongs to the Pax family of transcriptional factor genes. This gene is expressed in embryonic tissues such as somites, pharyngeal pouch endoderm, distal limb buds and neural crest-derived mesenchyme. Polymorphisms in the upstream promoter region of the human PAX9 have been associated with human non-syndromic tooth agenesis. In the present study, we verified the in vitro mRNA expression of this gene and the luciferase activity of two constructs containing promoter sequences of the PAX9 gene. MATERIAL AND METHODS: Embryonic tissues were obtained from digits, face, and midbrain/hindbrain regions. Fragments containing PAX9 promoter sequences were cloned into reporter plasmids and were transfected into the different cell cultures. mRNA were extracted from primary cell cultures. RESULTS: The semi-quantitative RT-PCR results showed that in vitro E13.5 limb bud and CNS cells express PAX9, but cells derived from the facial region do not. Moreover, the luciferase assay showed that protein activity of the constructed vector was weaker than pgl3 -basic alone. CONCLUSIONS: The present results suggest that the promoter sequences analyzed are not sufficient to drive PAX9 gene transcription. Universidade de São Paulo. Faculdade de Odontologia de Bauru2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/383010.1590/S1678-77572010000500009Journal of Applied Oral Science; Vol. 18 No. 5 (2010); 482-486 Journal of Applied Oral Science; Vol. 18 Núm. 5 (2010); 482-486 Journal of Applied Oral Science; v. 18 n. 5 (2010); 482-486 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3830/4520Copyright (c) 2010 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessAlmeida, Carolina Vieira deAndrade, Simone Caixeta deSaito, Cristiane Pereira BorgesRamenzoni, Liza LimaLine, Sergio Roberto Peres2012-04-27T12:10:50Zoai:revistas.usp.br:article/3830Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T12:10:50Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Transcriptional analysis of the human PAX9 promoter |
title |
Transcriptional analysis of the human PAX9 promoter |
spellingShingle |
Transcriptional analysis of the human PAX9 promoter Almeida, Carolina Vieira de PAX9 transcription factor Genetic promoter regions Anodontia |
title_short |
Transcriptional analysis of the human PAX9 promoter |
title_full |
Transcriptional analysis of the human PAX9 promoter |
title_fullStr |
Transcriptional analysis of the human PAX9 promoter |
title_full_unstemmed |
Transcriptional analysis of the human PAX9 promoter |
title_sort |
Transcriptional analysis of the human PAX9 promoter |
author |
Almeida, Carolina Vieira de |
author_facet |
Almeida, Carolina Vieira de Andrade, Simone Caixeta de Saito, Cristiane Pereira Borges Ramenzoni, Liza Lima Line, Sergio Roberto Peres |
author_role |
author |
author2 |
Andrade, Simone Caixeta de Saito, Cristiane Pereira Borges Ramenzoni, Liza Lima Line, Sergio Roberto Peres |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Almeida, Carolina Vieira de Andrade, Simone Caixeta de Saito, Cristiane Pereira Borges Ramenzoni, Liza Lima Line, Sergio Roberto Peres |
dc.subject.por.fl_str_mv |
PAX9 transcription factor Genetic promoter regions Anodontia |
topic |
PAX9 transcription factor Genetic promoter regions Anodontia |
description |
OBJECTIVES: PAX9 belongs to the Pax family of transcriptional factor genes. This gene is expressed in embryonic tissues such as somites, pharyngeal pouch endoderm, distal limb buds and neural crest-derived mesenchyme. Polymorphisms in the upstream promoter region of the human PAX9 have been associated with human non-syndromic tooth agenesis. In the present study, we verified the in vitro mRNA expression of this gene and the luciferase activity of two constructs containing promoter sequences of the PAX9 gene. MATERIAL AND METHODS: Embryonic tissues were obtained from digits, face, and midbrain/hindbrain regions. Fragments containing PAX9 promoter sequences were cloned into reporter plasmids and were transfected into the different cell cultures. mRNA were extracted from primary cell cultures. RESULTS: The semi-quantitative RT-PCR results showed that in vitro E13.5 limb bud and CNS cells express PAX9, but cells derived from the facial region do not. Moreover, the luciferase assay showed that protein activity of the constructed vector was weaker than pgl3 -basic alone. CONCLUSIONS: The present results suggest that the promoter sequences analyzed are not sufficient to drive PAX9 gene transcription. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3830 10.1590/S1678-77572010000500009 |
url |
https://www.revistas.usp.br/jaos/article/view/3830 |
identifier_str_mv |
10.1590/S1678-77572010000500009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3830/4520 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2010 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2010 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 18 No. 5 (2010); 482-486 Journal of Applied Oral Science; Vol. 18 Núm. 5 (2010); 482-486 Journal of Applied Oral Science; v. 18 n. 5 (2010); 482-486 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221676091211776 |