Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/187117 |
Resumo: | Objective: The goal of the present study was to determine the effect of systemic and topical ozone application on alveolar bone loss (ABL) by evaluating the effect of Hypoxia-inducible factor −1 alpha (HIF-1-α) and receptor activator of NF-kB ligand (RANKL)-positive cells on histopathological and immunohistochemical changes in a rat periodontitis model. Methodology: Thirty male Wistar rats were divided into three groups: 1) Group C (control group); 2) Group SO (systemic ozone group) and 3) Group TO (topical ozone group). Experimental periodontitis was induced with a 3/0 silk suture placed at the mandibular left first molars of rats, and the suture was removed 14 days later. Ozone gas was injected intraperitoneally (0.7 mg/kg) in SO group. Topical ozone application protocol was performed using an ozone generator at 80% concentration (4th grade) 90- degree probe for the duration of 30 s. Both ozone applications were carried out for two weeks at intervals of two days. Histomorphometric and immunohistochemical analysis were performed. Results:ABL was significantly lower in Group SO compared to Group C (p: 0.0052). HIF-1α- positive cells were significantly lower in Group TO than in Group C (p: 0.0043). RANKL-positive cells were significantly lower in Group SO and in Group TO compared to the control group (p: 0.0033, p: 0.0075, respectively). Conclusion: Both ozone applications decreased RANKL-positive cell counts, TO application decreased HIF-1-α positive cells counts, and SO application was found to be more effective in reducing ABL compared to control group. |
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Journal of applied oral science (Online) |
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Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in ratsBoneHypoxiaInflammationRank ligandOzoneObjective: The goal of the present study was to determine the effect of systemic and topical ozone application on alveolar bone loss (ABL) by evaluating the effect of Hypoxia-inducible factor −1 alpha (HIF-1-α) and receptor activator of NF-kB ligand (RANKL)-positive cells on histopathological and immunohistochemical changes in a rat periodontitis model. Methodology: Thirty male Wistar rats were divided into three groups: 1) Group C (control group); 2) Group SO (systemic ozone group) and 3) Group TO (topical ozone group). Experimental periodontitis was induced with a 3/0 silk suture placed at the mandibular left first molars of rats, and the suture was removed 14 days later. Ozone gas was injected intraperitoneally (0.7 mg/kg) in SO group. Topical ozone application protocol was performed using an ozone generator at 80% concentration (4th grade) 90- degree probe for the duration of 30 s. Both ozone applications were carried out for two weeks at intervals of two days. Histomorphometric and immunohistochemical analysis were performed. Results:ABL was significantly lower in Group SO compared to Group C (p: 0.0052). HIF-1α- positive cells were significantly lower in Group TO than in Group C (p: 0.0043). RANKL-positive cells were significantly lower in Group SO and in Group TO compared to the control group (p: 0.0033, p: 0.0075, respectively). Conclusion: Both ozone applications decreased RANKL-positive cell counts, TO application decreased HIF-1-α positive cells counts, and SO application was found to be more effective in reducing ABL compared to control group.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-06-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/18711710.1590/1678-7757-2019-0140Journal of Applied Oral Science; Vol. 28 (2020); e20190140Journal of Applied Oral Science; Vol. 28 (2020); e20190140Journal of Applied Oral Science; v. 28 (2020); e201901401678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/187117/172850Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSaglam, Ebru Alinca, Suzan Bayer Celik, Tugba Zengin Hacisalihoglu, Uguray Payam Dogan, Mehmet Ali 2021-06-14T17:19:32Zoai:revistas.usp.br:article/187117Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-06-14T17:19:32Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats |
title |
Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats |
spellingShingle |
Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats Saglam, Ebru Bone Hypoxia Inflammation Rank ligand Ozone |
title_short |
Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats |
title_full |
Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats |
title_fullStr |
Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats |
title_full_unstemmed |
Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats |
title_sort |
Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats |
author |
Saglam, Ebru |
author_facet |
Saglam, Ebru Alinca, Suzan Bayer Celik, Tugba Zengin Hacisalihoglu, Uguray Payam Dogan, Mehmet Ali |
author_role |
author |
author2 |
Alinca, Suzan Bayer Celik, Tugba Zengin Hacisalihoglu, Uguray Payam Dogan, Mehmet Ali |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Saglam, Ebru Alinca, Suzan Bayer Celik, Tugba Zengin Hacisalihoglu, Uguray Payam Dogan, Mehmet Ali |
dc.subject.por.fl_str_mv |
Bone Hypoxia Inflammation Rank ligand Ozone |
topic |
Bone Hypoxia Inflammation Rank ligand Ozone |
description |
Objective: The goal of the present study was to determine the effect of systemic and topical ozone application on alveolar bone loss (ABL) by evaluating the effect of Hypoxia-inducible factor −1 alpha (HIF-1-α) and receptor activator of NF-kB ligand (RANKL)-positive cells on histopathological and immunohistochemical changes in a rat periodontitis model. Methodology: Thirty male Wistar rats were divided into three groups: 1) Group C (control group); 2) Group SO (systemic ozone group) and 3) Group TO (topical ozone group). Experimental periodontitis was induced with a 3/0 silk suture placed at the mandibular left first molars of rats, and the suture was removed 14 days later. Ozone gas was injected intraperitoneally (0.7 mg/kg) in SO group. Topical ozone application protocol was performed using an ozone generator at 80% concentration (4th grade) 90- degree probe for the duration of 30 s. Both ozone applications were carried out for two weeks at intervals of two days. Histomorphometric and immunohistochemical analysis were performed. Results:ABL was significantly lower in Group SO compared to Group C (p: 0.0052). HIF-1α- positive cells were significantly lower in Group TO than in Group C (p: 0.0043). RANKL-positive cells were significantly lower in Group SO and in Group TO compared to the control group (p: 0.0033, p: 0.0075, respectively). Conclusion: Both ozone applications decreased RANKL-positive cell counts, TO application decreased HIF-1-α positive cells counts, and SO application was found to be more effective in reducing ABL compared to control group. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/187117 10.1590/1678-7757-2019-0140 |
url |
https://www.revistas.usp.br/jaos/article/view/187117 |
identifier_str_mv |
10.1590/1678-7757-2019-0140 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/187117/172850 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 28 (2020); e20190140 Journal of Applied Oral Science; Vol. 28 (2020); e20190140 Journal of Applied Oral Science; v. 28 (2020); e20190140 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221681235525632 |