Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | https://www.revistas.usp.br/sa/article/view/100187 |
Resumo: | Zucchini yellow mosaic virus (ZYMV) causes substantial economic losses in cucurbit crops. Although ZYMV has been present in Brazil for more than 20 years, there is little information about the biological and molecular characteristics of the isolates found in the country. This study aimed to characterize the experimental hosts, pathotypes and genetic diversity of a collection of eleven Brazilian ZYMV isolates within the coat protein gene. For biological analysis, plant species from Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Solanaceae, and Pedaliaceae were mechanically inoculated and pathotypes were identified based on the reaction of a resistant Cucumis melo, accession PI414723. All of the cucurbit species/varieties and Sesamum indicum were systemically infected with all isolates. The nucleotide sequence variability of the coat protein gene ranged from 82 % to 99 % compared to the corresponding sequences of ZYMV isolates from different geographical locations. No recombination event was detected in the coat protein gene of the isolates. |
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Scientia Agrícola (Online) |
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Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus Zucchini yellow mosaic virus (ZYMV) causes substantial economic losses in cucurbit crops. Although ZYMV has been present in Brazil for more than 20 years, there is little information about the biological and molecular characteristics of the isolates found in the country. This study aimed to characterize the experimental hosts, pathotypes and genetic diversity of a collection of eleven Brazilian ZYMV isolates within the coat protein gene. For biological analysis, plant species from Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Solanaceae, and Pedaliaceae were mechanically inoculated and pathotypes were identified based on the reaction of a resistant Cucumis melo, accession PI414723. All of the cucurbit species/varieties and Sesamum indicum were systemically infected with all isolates. The nucleotide sequence variability of the coat protein gene ranged from 82 % to 99 % compared to the corresponding sequences of ZYMV isolates from different geographical locations. No recombination event was detected in the coat protein gene of the isolates. Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz2015-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/10018710.1590/0103-9016-2014-0197Scientia Agricola; v. 72 n. 2 (2015); 187-191Scientia Agricola; Vol. 72 Núm. 2 (2015); 187-191Scientia Agricola; Vol. 72 No. 2 (2015); 187-1911678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/sa/article/view/100187/98849Copyright (c) 2015 Scientia Agricolainfo:eu-repo/semantics/openAccessSpadotti, David Marques de Almeida Wassano, Débora Targino Rezende, Jorge Alberto Marques Camargo, Luis Eduardo Aranha Inoue-Nagata, Alice Kazuko 2015-08-31T12:01:38Zoai:revistas.usp.br:article/100187Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2015-08-31T12:01:38Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus |
title |
Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus |
spellingShingle |
Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus Spadotti, David Marques de Almeida |
title_short |
Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus |
title_full |
Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus |
title_fullStr |
Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus |
title_full_unstemmed |
Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus |
title_sort |
Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus |
author |
Spadotti, David Marques de Almeida |
author_facet |
Spadotti, David Marques de Almeida Wassano, Débora Targino Rezende, Jorge Alberto Marques Camargo, Luis Eduardo Aranha Inoue-Nagata, Alice Kazuko |
author_role |
author |
author2 |
Wassano, Débora Targino Rezende, Jorge Alberto Marques Camargo, Luis Eduardo Aranha Inoue-Nagata, Alice Kazuko |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Spadotti, David Marques de Almeida Wassano, Débora Targino Rezende, Jorge Alberto Marques Camargo, Luis Eduardo Aranha Inoue-Nagata, Alice Kazuko |
description |
Zucchini yellow mosaic virus (ZYMV) causes substantial economic losses in cucurbit crops. Although ZYMV has been present in Brazil for more than 20 years, there is little information about the biological and molecular characteristics of the isolates found in the country. This study aimed to characterize the experimental hosts, pathotypes and genetic diversity of a collection of eleven Brazilian ZYMV isolates within the coat protein gene. For biological analysis, plant species from Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Solanaceae, and Pedaliaceae were mechanically inoculated and pathotypes were identified based on the reaction of a resistant Cucumis melo, accession PI414723. All of the cucurbit species/varieties and Sesamum indicum were systemically infected with all isolates. The nucleotide sequence variability of the coat protein gene ranged from 82 % to 99 % compared to the corresponding sequences of ZYMV isolates from different geographical locations. No recombination event was detected in the coat protein gene of the isolates. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/100187 10.1590/0103-9016-2014-0197 |
url |
https://www.revistas.usp.br/sa/article/view/100187 |
identifier_str_mv |
10.1590/0103-9016-2014-0197 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/100187/98849 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Scientia Agricola info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Scientia Agricola |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
dc.source.none.fl_str_mv |
Scientia Agricola; v. 72 n. 2 (2015); 187-191 Scientia Agricola; Vol. 72 Núm. 2 (2015); 187-191 Scientia Agricola; Vol. 72 No. 2 (2015); 187-191 1678-992X 0103-9016 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1800222792444018688 |