Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus

Detalhes bibliográficos
Autor(a) principal: Spadotti, David Marques de Almeida
Data de Publicação: 2015
Outros Autores: Wassano, Débora Targino, Rezende, Jorge Alberto Marques, Camargo, Luis Eduardo Aranha, Inoue-Nagata, Alice Kazuko
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: https://www.revistas.usp.br/sa/article/view/100187
Resumo: Zucchini yellow mosaic virus (ZYMV) causes substantial economic losses in cucurbit crops. Although ZYMV has been present in Brazil for more than 20 years, there is little information about the biological and molecular characteristics of the isolates found in the country. This study aimed to characterize the experimental hosts, pathotypes and genetic diversity of a collection of eleven Brazilian ZYMV isolates within the coat protein gene. For biological analysis, plant species from Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Solanaceae, and Pedaliaceae were mechanically inoculated and pathotypes were identified based on the reaction of a resistant Cucumis melo, accession PI414723. All of the cucurbit species/varieties and Sesamum indicum were systemically infected with all isolates. The nucleotide sequence variability of the coat protein gene ranged from 82 % to 99 % compared to the corresponding sequences of ZYMV isolates from different geographical locations. No recombination event was detected in the coat protein gene of the isolates.
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spelling Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus Zucchini yellow mosaic virus (ZYMV) causes substantial economic losses in cucurbit crops. Although ZYMV has been present in Brazil for more than 20 years, there is little information about the biological and molecular characteristics of the isolates found in the country. This study aimed to characterize the experimental hosts, pathotypes and genetic diversity of a collection of eleven Brazilian ZYMV isolates within the coat protein gene. For biological analysis, plant species from Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Solanaceae, and Pedaliaceae were mechanically inoculated and pathotypes were identified based on the reaction of a resistant Cucumis melo, accession PI414723. All of the cucurbit species/varieties and Sesamum indicum were systemically infected with all isolates. The nucleotide sequence variability of the coat protein gene ranged from 82 % to 99 % compared to the corresponding sequences of ZYMV isolates from different geographical locations. No recombination event was detected in the coat protein gene of the isolates. Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz2015-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/10018710.1590/0103-9016-2014-0197Scientia Agricola; v. 72 n. 2 (2015); 187-191Scientia Agricola; Vol. 72 Núm. 2 (2015); 187-191Scientia Agricola; Vol. 72 No. 2 (2015); 187-1911678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/sa/article/view/100187/98849Copyright (c) 2015 Scientia Agricolainfo:eu-repo/semantics/openAccessSpadotti, David Marques de Almeida Wassano, Débora Targino Rezende, Jorge Alberto Marques Camargo, Luis Eduardo Aranha Inoue-Nagata, Alice Kazuko 2015-08-31T12:01:38Zoai:revistas.usp.br:article/100187Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2015-08-31T12:01:38Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus
title Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus
spellingShingle Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus
Spadotti, David Marques de Almeida
title_short Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus
title_full Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus
title_fullStr Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus
title_full_unstemmed Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus
title_sort Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus
author Spadotti, David Marques de Almeida
author_facet Spadotti, David Marques de Almeida
Wassano, Débora Targino
Rezende, Jorge Alberto Marques
Camargo, Luis Eduardo Aranha
Inoue-Nagata, Alice Kazuko
author_role author
author2 Wassano, Débora Targino
Rezende, Jorge Alberto Marques
Camargo, Luis Eduardo Aranha
Inoue-Nagata, Alice Kazuko
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Spadotti, David Marques de Almeida
Wassano, Débora Targino
Rezende, Jorge Alberto Marques
Camargo, Luis Eduardo Aranha
Inoue-Nagata, Alice Kazuko
description Zucchini yellow mosaic virus (ZYMV) causes substantial economic losses in cucurbit crops. Although ZYMV has been present in Brazil for more than 20 years, there is little information about the biological and molecular characteristics of the isolates found in the country. This study aimed to characterize the experimental hosts, pathotypes and genetic diversity of a collection of eleven Brazilian ZYMV isolates within the coat protein gene. For biological analysis, plant species from Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Solanaceae, and Pedaliaceae were mechanically inoculated and pathotypes were identified based on the reaction of a resistant Cucumis melo, accession PI414723. All of the cucurbit species/varieties and Sesamum indicum were systemically infected with all isolates. The nucleotide sequence variability of the coat protein gene ranged from 82 % to 99 % compared to the corresponding sequences of ZYMV isolates from different geographical locations. No recombination event was detected in the coat protein gene of the isolates.
publishDate 2015
dc.date.none.fl_str_mv 2015-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/sa/article/view/100187
10.1590/0103-9016-2014-0197
url https://www.revistas.usp.br/sa/article/view/100187
identifier_str_mv 10.1590/0103-9016-2014-0197
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/sa/article/view/100187/98849
dc.rights.driver.fl_str_mv Copyright (c) 2015 Scientia Agricola
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2015 Scientia Agricola
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
publisher.none.fl_str_mv Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
dc.source.none.fl_str_mv Scientia Agricola; v. 72 n. 2 (2015); 187-191
Scientia Agricola; Vol. 72 Núm. 2 (2015); 187-191
Scientia Agricola; Vol. 72 No. 2 (2015); 187-191
1678-992X
0103-9016
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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