Sesame phyllody associated with a 16SrI-B phytoplasma, a ‘Candidatus Phytoplasma asteris’-related strain, in Paraguay

Detalhes bibliográficos
Autor(a) principal: Ganem Junior,Evandro de Jesus
Data de Publicação: 2019
Outros Autores: Segnana,Luis Gonzalez, Kitajima,Elliot Watanabe, Bedendo,Ivan Paulo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019000100047
Resumo: ABSTRACT: Sesame (Sesamum indicum L.) plants exhibiting symptoms of phyllody disease were observed in commercial fields in Paraguay. The symptoms were indicative of infection by phytoplasmas. Thus, the present study investigated the association between affected plants and phytoplasma, which was later analyzed using molecular and phylogenetic methods. Total DNA was extracted from symptomatic and asymptomatic plants and used in nested PCR assays using primers R16SN910601/R16SN011119 and R16F2n/16R2. Amplified products of 1.2 Kb revealed the presence of phytoplasma in all diseased plants, and electron microscopy confirmed the presence of phytoplasmas within phloem vessels. Nucleotide sequences from sesame phytoplasma shared 99 % similarity with phytoplasmas belonging to group 16SrI. Computer-simulated RFLP indicated that the detected phytoplasma is representative of the 16SrI-B, therefore, a ‘Candidatus Phytoplasma asteris’-related strain. Phylogenetic analysis was in agreement with virtual RFLP. Our findings expand the current knowledge regarding distribution of representatives of the aster yellows group in a new agroecosystem and implicate sesame as a new host of 16SrI-B phytoplasma in Latin America.
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spelling Sesame phyllody associated with a 16SrI-B phytoplasma, a ‘Candidatus Phytoplasma asteris’-related strain, in ParaguaySesamum indicum (L.)Mollicutesyellowsphloem bacteriaABSTRACT: Sesame (Sesamum indicum L.) plants exhibiting symptoms of phyllody disease were observed in commercial fields in Paraguay. The symptoms were indicative of infection by phytoplasmas. Thus, the present study investigated the association between affected plants and phytoplasma, which was later analyzed using molecular and phylogenetic methods. Total DNA was extracted from symptomatic and asymptomatic plants and used in nested PCR assays using primers R16SN910601/R16SN011119 and R16F2n/16R2. Amplified products of 1.2 Kb revealed the presence of phytoplasma in all diseased plants, and electron microscopy confirmed the presence of phytoplasmas within phloem vessels. Nucleotide sequences from sesame phytoplasma shared 99 % similarity with phytoplasmas belonging to group 16SrI. Computer-simulated RFLP indicated that the detected phytoplasma is representative of the 16SrI-B, therefore, a ‘Candidatus Phytoplasma asteris’-related strain. Phylogenetic analysis was in agreement with virtual RFLP. Our findings expand the current knowledge regarding distribution of representatives of the aster yellows group in a new agroecosystem and implicate sesame as a new host of 16SrI-B phytoplasma in Latin America.Escola Superior de Agricultura "Luiz de Queiroz"2019-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019000100047Scientia Agricola v.76 n.1 2019reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-992x-2017-0140info:eu-repo/semantics/openAccessGanem Junior,Evandro de JesusSegnana,Luis GonzalezKitajima,Elliot WatanabeBedendo,Ivan Pauloeng2018-10-19T00:00:00Zoai:scielo:S0103-90162019000100047Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2018-10-19T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Sesame phyllody associated with a 16SrI-B phytoplasma, a ‘Candidatus Phytoplasma asteris’-related strain, in Paraguay
title Sesame phyllody associated with a 16SrI-B phytoplasma, a ‘Candidatus Phytoplasma asteris’-related strain, in Paraguay
spellingShingle Sesame phyllody associated with a 16SrI-B phytoplasma, a ‘Candidatus Phytoplasma asteris’-related strain, in Paraguay
Ganem Junior,Evandro de Jesus
Sesamum indicum (L.)
Mollicutes
yellows
phloem bacteria
title_short Sesame phyllody associated with a 16SrI-B phytoplasma, a ‘Candidatus Phytoplasma asteris’-related strain, in Paraguay
title_full Sesame phyllody associated with a 16SrI-B phytoplasma, a ‘Candidatus Phytoplasma asteris’-related strain, in Paraguay
title_fullStr Sesame phyllody associated with a 16SrI-B phytoplasma, a ‘Candidatus Phytoplasma asteris’-related strain, in Paraguay
title_full_unstemmed Sesame phyllody associated with a 16SrI-B phytoplasma, a ‘Candidatus Phytoplasma asteris’-related strain, in Paraguay
title_sort Sesame phyllody associated with a 16SrI-B phytoplasma, a ‘Candidatus Phytoplasma asteris’-related strain, in Paraguay
author Ganem Junior,Evandro de Jesus
author_facet Ganem Junior,Evandro de Jesus
Segnana,Luis Gonzalez
Kitajima,Elliot Watanabe
Bedendo,Ivan Paulo
author_role author
author2 Segnana,Luis Gonzalez
Kitajima,Elliot Watanabe
Bedendo,Ivan Paulo
author2_role author
author
author
dc.contributor.author.fl_str_mv Ganem Junior,Evandro de Jesus
Segnana,Luis Gonzalez
Kitajima,Elliot Watanabe
Bedendo,Ivan Paulo
dc.subject.por.fl_str_mv Sesamum indicum (L.)
Mollicutes
yellows
phloem bacteria
topic Sesamum indicum (L.)
Mollicutes
yellows
phloem bacteria
description ABSTRACT: Sesame (Sesamum indicum L.) plants exhibiting symptoms of phyllody disease were observed in commercial fields in Paraguay. The symptoms were indicative of infection by phytoplasmas. Thus, the present study investigated the association between affected plants and phytoplasma, which was later analyzed using molecular and phylogenetic methods. Total DNA was extracted from symptomatic and asymptomatic plants and used in nested PCR assays using primers R16SN910601/R16SN011119 and R16F2n/16R2. Amplified products of 1.2 Kb revealed the presence of phytoplasma in all diseased plants, and electron microscopy confirmed the presence of phytoplasmas within phloem vessels. Nucleotide sequences from sesame phytoplasma shared 99 % similarity with phytoplasmas belonging to group 16SrI. Computer-simulated RFLP indicated that the detected phytoplasma is representative of the 16SrI-B, therefore, a ‘Candidatus Phytoplasma asteris’-related strain. Phylogenetic analysis was in agreement with virtual RFLP. Our findings expand the current knowledge regarding distribution of representatives of the aster yellows group in a new agroecosystem and implicate sesame as a new host of 16SrI-B phytoplasma in Latin America.
publishDate 2019
dc.date.none.fl_str_mv 2019-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019000100047
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019000100047
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-992x-2017-0140
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.76 n.1 2019
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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