Phytoplasma of 16SrVII-B subgroup associated to shoot proliferation in Physalis peruviana plants

Detalhes bibliográficos
Autor(a) principal: Ferreira,Jacson
Data de Publicação: 2022
Outros Autores: Almeida,Cláudia Alves de, Oliveira,Felipe Franco de, Fariña,Arnaldo, Kitajima,Elliot Watanabe, Bedendo,Ivan Paulo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162022000501201
Resumo: ABSTRACT: Physalis is an herbaceous species native to the Andes region. Currently, it is cultivated in various Brazilian states due to the economic interest of growers for this new fruit. Physalis plants grown in the field showed symptoms of shoot proliferation, leaf malformation, and chlorosis. Since these symptoms are commonly induced by phytoplasmas, this study investigated to confirm the presence of these prokaryotes in symptomatic plants. After DNA extraction from symptomatic and asymptomatic plants, phytoplasmas were found in all affected plants through the nested PCR. Examination by transmission electron microscopy (TEM) using appropriately prepared segments of leaf veins allowed the visualization of typical pleomorphic cells of phytoplasmas in the phloem of symptomatic plants. The computer-simulated RFLP patterns and the phylogenetic analysis allowed identifying the detected phytoplasmas as a ‘Candidatus Phytoplasma fraxini’-related strain belonging to the 16SrVII-B subgroup. Moreover, physalis was identified as an additional host species for phytoplasmas in the 16SrVII group, expanding the current knowledge on the host range of phytoplasmas in this group.
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spelling Phytoplasma of 16SrVII-B subgroup associated to shoot proliferation in Physalis peruviana plantsMollicutesphloem bacteriacape-gooseberryyellowsABSTRACT: Physalis is an herbaceous species native to the Andes region. Currently, it is cultivated in various Brazilian states due to the economic interest of growers for this new fruit. Physalis plants grown in the field showed symptoms of shoot proliferation, leaf malformation, and chlorosis. Since these symptoms are commonly induced by phytoplasmas, this study investigated to confirm the presence of these prokaryotes in symptomatic plants. After DNA extraction from symptomatic and asymptomatic plants, phytoplasmas were found in all affected plants through the nested PCR. Examination by transmission electron microscopy (TEM) using appropriately prepared segments of leaf veins allowed the visualization of typical pleomorphic cells of phytoplasmas in the phloem of symptomatic plants. The computer-simulated RFLP patterns and the phylogenetic analysis allowed identifying the detected phytoplasmas as a ‘Candidatus Phytoplasma fraxini’-related strain belonging to the 16SrVII-B subgroup. Moreover, physalis was identified as an additional host species for phytoplasmas in the 16SrVII group, expanding the current knowledge on the host range of phytoplasmas in this group.Escola Superior de Agricultura "Luiz de Queiroz"2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162022000501201Scientia Agricola v.79 n.5 2022reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-992x-2020-0277info:eu-repo/semantics/openAccessFerreira,JacsonAlmeida,Cláudia Alves deOliveira,Felipe Franco deFariña,ArnaldoKitajima,Elliot WatanabeBedendo,Ivan Pauloeng2021-09-03T00:00:00Zoai:scielo:S0103-90162022000501201Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2021-09-03T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Phytoplasma of 16SrVII-B subgroup associated to shoot proliferation in Physalis peruviana plants
title Phytoplasma of 16SrVII-B subgroup associated to shoot proliferation in Physalis peruviana plants
spellingShingle Phytoplasma of 16SrVII-B subgroup associated to shoot proliferation in Physalis peruviana plants
Ferreira,Jacson
Mollicutes
phloem bacteria
cape-gooseberry
yellows
title_short Phytoplasma of 16SrVII-B subgroup associated to shoot proliferation in Physalis peruviana plants
title_full Phytoplasma of 16SrVII-B subgroup associated to shoot proliferation in Physalis peruviana plants
title_fullStr Phytoplasma of 16SrVII-B subgroup associated to shoot proliferation in Physalis peruviana plants
title_full_unstemmed Phytoplasma of 16SrVII-B subgroup associated to shoot proliferation in Physalis peruviana plants
title_sort Phytoplasma of 16SrVII-B subgroup associated to shoot proliferation in Physalis peruviana plants
author Ferreira,Jacson
author_facet Ferreira,Jacson
Almeida,Cláudia Alves de
Oliveira,Felipe Franco de
Fariña,Arnaldo
Kitajima,Elliot Watanabe
Bedendo,Ivan Paulo
author_role author
author2 Almeida,Cláudia Alves de
Oliveira,Felipe Franco de
Fariña,Arnaldo
Kitajima,Elliot Watanabe
Bedendo,Ivan Paulo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira,Jacson
Almeida,Cláudia Alves de
Oliveira,Felipe Franco de
Fariña,Arnaldo
Kitajima,Elliot Watanabe
Bedendo,Ivan Paulo
dc.subject.por.fl_str_mv Mollicutes
phloem bacteria
cape-gooseberry
yellows
topic Mollicutes
phloem bacteria
cape-gooseberry
yellows
description ABSTRACT: Physalis is an herbaceous species native to the Andes region. Currently, it is cultivated in various Brazilian states due to the economic interest of growers for this new fruit. Physalis plants grown in the field showed symptoms of shoot proliferation, leaf malformation, and chlorosis. Since these symptoms are commonly induced by phytoplasmas, this study investigated to confirm the presence of these prokaryotes in symptomatic plants. After DNA extraction from symptomatic and asymptomatic plants, phytoplasmas were found in all affected plants through the nested PCR. Examination by transmission electron microscopy (TEM) using appropriately prepared segments of leaf veins allowed the visualization of typical pleomorphic cells of phytoplasmas in the phloem of symptomatic plants. The computer-simulated RFLP patterns and the phylogenetic analysis allowed identifying the detected phytoplasmas as a ‘Candidatus Phytoplasma fraxini’-related strain belonging to the 16SrVII-B subgroup. Moreover, physalis was identified as an additional host species for phytoplasmas in the 16SrVII group, expanding the current knowledge on the host range of phytoplasmas in this group.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162022000501201
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162022000501201
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-992x-2020-0277
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.79 n.5 2022
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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