Embryogenic calli induction from nucellar tissue of Citrus cultivars
Autor(a) principal: | |
---|---|
Data de Publicação: | 1999 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000500012 |
Resumo: | Nucellar tissues of seven Citrus varieties were introduced onto three growth media to produce embryogenic callus. The media tested were: EME [MT, modified, with the addition of malt extract (500 mg.L-1)]; 1/2-EME [half concentration of MT macronutrients + half concentration of BH3 macronutrients + 500 mg.L-1 malt extract + 1.55 g.L-1 of glutamine]; and EBA [EME + 0.44 muM 6-benzyladenine + 0.04 muM 2,4 D]. Soft friable calli were obtained from 'Cravo' and 'Ponkan' mandarins (Citrus reticulata, Blanco), 'Murcott' tangor (Citrus reticulata Blanco x Citrus sinensis L. Osbeck), 'Serra d'água' and 'Valencia' sweet oranges (Citrus sinensis, L. Osbeck) 120 days after callus induction. 'Natal' and 'Pera' sweet oranges (Citrus sinensis, L. Osbeck) produced hard non-friable calli in this period. EME and 1/2-EME media had the best results for 'Cravo' mandarin, 'Ponkan' mandarin and 'Serra d'água' sweet orange, whereas EBA was the best media composition to induce soft friable calli on 'Murcott' tangor and 'Valencia' sweet orange. Friable callus cultures of 'Cravo' and 'Ponkan' mandarins, and 'Murcott' tangor yielded high quality protoplasts after isolation. Abbreviations: a.c. - activated charcoal; BA - 6-benzyladenine; IAA - indole-acetic acid; 2,4-D - 2,4-diclorophenoxyacetic acid; MT - Murashige & Tucker basal medium. |
id |
USP-18_39bb7d8dfb394a8ac00223e20893218d |
---|---|
oai_identifier_str |
oai:scielo:S0103-90161999000500012 |
network_acronym_str |
USP-18 |
network_name_str |
Scientia Agrícola (Online) |
repository_id_str |
|
spelling |
Embryogenic calli induction from nucellar tissue of Citrus cultivarsbiotechnologytissue culturecultivar improvementgrowth regulatorprotoplastNucellar tissues of seven Citrus varieties were introduced onto three growth media to produce embryogenic callus. The media tested were: EME [MT, modified, with the addition of malt extract (500 mg.L-1)]; 1/2-EME [half concentration of MT macronutrients + half concentration of BH3 macronutrients + 500 mg.L-1 malt extract + 1.55 g.L-1 of glutamine]; and EBA [EME + 0.44 muM 6-benzyladenine + 0.04 muM 2,4 D]. Soft friable calli were obtained from 'Cravo' and 'Ponkan' mandarins (Citrus reticulata, Blanco), 'Murcott' tangor (Citrus reticulata Blanco x Citrus sinensis L. Osbeck), 'Serra d'água' and 'Valencia' sweet oranges (Citrus sinensis, L. Osbeck) 120 days after callus induction. 'Natal' and 'Pera' sweet oranges (Citrus sinensis, L. Osbeck) produced hard non-friable calli in this period. EME and 1/2-EME media had the best results for 'Cravo' mandarin, 'Ponkan' mandarin and 'Serra d'água' sweet orange, whereas EBA was the best media composition to induce soft friable calli on 'Murcott' tangor and 'Valencia' sweet orange. Friable callus cultures of 'Cravo' and 'Ponkan' mandarins, and 'Murcott' tangor yielded high quality protoplasts after isolation. Abbreviations: a.c. - activated charcoal; BA - 6-benzyladenine; IAA - indole-acetic acid; 2,4-D - 2,4-diclorophenoxyacetic acid; MT - Murashige & Tucker basal medium.Escola Superior de Agricultura "Luiz de Queiroz"1999-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000500012Scientia Agricola v.56 n.4 suppl.0 1999reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90161999000500012info:eu-repo/semantics/openAccessMendes-da-Glória,Fernanda JanuzziMourão Filho,Francisco de Assis AlvesDemétrio,Clarice Garcia BorgesMendes,Beatriz Madalena Januzzieng2000-12-22T00:00:00Zoai:scielo:S0103-90161999000500012Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2000-12-22T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Embryogenic calli induction from nucellar tissue of Citrus cultivars |
title |
Embryogenic calli induction from nucellar tissue of Citrus cultivars |
spellingShingle |
Embryogenic calli induction from nucellar tissue of Citrus cultivars Mendes-da-Glória,Fernanda Januzzi biotechnology tissue culture cultivar improvement growth regulator protoplast |
title_short |
Embryogenic calli induction from nucellar tissue of Citrus cultivars |
title_full |
Embryogenic calli induction from nucellar tissue of Citrus cultivars |
title_fullStr |
Embryogenic calli induction from nucellar tissue of Citrus cultivars |
title_full_unstemmed |
Embryogenic calli induction from nucellar tissue of Citrus cultivars |
title_sort |
Embryogenic calli induction from nucellar tissue of Citrus cultivars |
author |
Mendes-da-Glória,Fernanda Januzzi |
author_facet |
Mendes-da-Glória,Fernanda Januzzi Mourão Filho,Francisco de Assis Alves Demétrio,Clarice Garcia Borges Mendes,Beatriz Madalena Januzzi |
author_role |
author |
author2 |
Mourão Filho,Francisco de Assis Alves Demétrio,Clarice Garcia Borges Mendes,Beatriz Madalena Januzzi |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Mendes-da-Glória,Fernanda Januzzi Mourão Filho,Francisco de Assis Alves Demétrio,Clarice Garcia Borges Mendes,Beatriz Madalena Januzzi |
dc.subject.por.fl_str_mv |
biotechnology tissue culture cultivar improvement growth regulator protoplast |
topic |
biotechnology tissue culture cultivar improvement growth regulator protoplast |
description |
Nucellar tissues of seven Citrus varieties were introduced onto three growth media to produce embryogenic callus. The media tested were: EME [MT, modified, with the addition of malt extract (500 mg.L-1)]; 1/2-EME [half concentration of MT macronutrients + half concentration of BH3 macronutrients + 500 mg.L-1 malt extract + 1.55 g.L-1 of glutamine]; and EBA [EME + 0.44 muM 6-benzyladenine + 0.04 muM 2,4 D]. Soft friable calli were obtained from 'Cravo' and 'Ponkan' mandarins (Citrus reticulata, Blanco), 'Murcott' tangor (Citrus reticulata Blanco x Citrus sinensis L. Osbeck), 'Serra d'água' and 'Valencia' sweet oranges (Citrus sinensis, L. Osbeck) 120 days after callus induction. 'Natal' and 'Pera' sweet oranges (Citrus sinensis, L. Osbeck) produced hard non-friable calli in this period. EME and 1/2-EME media had the best results for 'Cravo' mandarin, 'Ponkan' mandarin and 'Serra d'água' sweet orange, whereas EBA was the best media composition to induce soft friable calli on 'Murcott' tangor and 'Valencia' sweet orange. Friable callus cultures of 'Cravo' and 'Ponkan' mandarins, and 'Murcott' tangor yielded high quality protoplasts after isolation. Abbreviations: a.c. - activated charcoal; BA - 6-benzyladenine; IAA - indole-acetic acid; 2,4-D - 2,4-diclorophenoxyacetic acid; MT - Murashige & Tucker basal medium. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000500012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000500012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90161999000500012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.56 n.4 suppl.0 1999 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936456953397248 |