Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck

Detalhes bibliográficos
Autor(a) principal: Calixto, Marcia Cristina
Data de Publicação: 2004
Outros Autores: Mourão Filho, Francisco de Assis Alves, Mendes, Beatriz Madalena Januzzi, Vieira, Maria Lúcia Carneiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6831
Resumo: This work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of ‘Hamlin’ sweet orange + ‘Indian Red’ pummelo and ‘Hamlin’ sweet orange + ‘Singapura’ pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.
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spelling Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) OsbeckHibridação somática entre Citrus sinensis (L.) Osbeck e C. grandis (L.) Osbeckgenetic improvement; protoplast fusion; rootstockmelhoramento genético; fusão de protoplastos; porta-enxertoThis work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of ‘Hamlin’ sweet orange + ‘Indian Red’ pummelo and ‘Hamlin’ sweet orange + ‘Singapura’ pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.O objetivo deste trabalho foi produzir híbridos somáticos entre laranjas doces e toranjas. Após fusão de protoplastos embriogênicos de laranja doce com protoplastos derivados de mesófilo foliar de toranjas, plantas foram regeneradas por embriogênese somática e aclimatizadas em casa de vegetação. Os híbridos laranja ‘Hamlin’ + toranja ‘Indian Red’ e laranja ‘Hamlin’ + toranja ‘Singapura’ foram confirmados pela morfologia foliar, contagem do número de cromossomos e análise molecular. Esses híbridos apresentam potencial para serem testados como porta-enxertos tolerantes a declínio, tristeza e doenças causadas por Phytophthora, bem como em programas de melhoramento de porta-enxertos.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCalixto, Marcia CristinaMourão Filho, Francisco de Assis AlvesMendes, Beatriz Madalena JanuzziVieira, Maria Lúcia Carneiro2004-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6831Pesquisa Agropecuaria Brasileira; v.39, n.7, jul. 2004; 721-724Pesquisa Agropecuária Brasileira; v.39, n.7, jul. 2004; 721-7241678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/6831/3887info:eu-repo/semantics/openAccess2014-08-05T17:50:28Zoai:ojs.seer.sct.embrapa.br:article/6831Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-08-05T17:50:28Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck
Hibridação somática entre Citrus sinensis (L.) Osbeck e C. grandis (L.) Osbeck
title Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck
spellingShingle Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck
Calixto, Marcia Cristina
genetic improvement; protoplast fusion; rootstock
melhoramento genético; fusão de protoplastos; porta-enxerto
title_short Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck
title_full Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck
title_fullStr Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck
title_full_unstemmed Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck
title_sort Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck
author Calixto, Marcia Cristina
author_facet Calixto, Marcia Cristina
Mourão Filho, Francisco de Assis Alves
Mendes, Beatriz Madalena Januzzi
Vieira, Maria Lúcia Carneiro
author_role author
author2 Mourão Filho, Francisco de Assis Alves
Mendes, Beatriz Madalena Januzzi
Vieira, Maria Lúcia Carneiro
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Calixto, Marcia Cristina
Mourão Filho, Francisco de Assis Alves
Mendes, Beatriz Madalena Januzzi
Vieira, Maria Lúcia Carneiro
dc.subject.por.fl_str_mv genetic improvement; protoplast fusion; rootstock
melhoramento genético; fusão de protoplastos; porta-enxerto
topic genetic improvement; protoplast fusion; rootstock
melhoramento genético; fusão de protoplastos; porta-enxerto
description This work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of ‘Hamlin’ sweet orange + ‘Indian Red’ pummelo and ‘Hamlin’ sweet orange + ‘Singapura’ pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.
publishDate 2004
dc.date.none.fl_str_mv 2004-07-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6831
url https://seer.sct.embrapa.br/index.php/pab/article/view/6831
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6831/3887
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.39, n.7, jul. 2004; 721-724
Pesquisa Agropecuária Brasileira; v.39, n.7, jul. 2004; 721-724
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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