Interactions of adjuvants on adhesion and germination of Isaria fumosorosea on adults of Diaphorina citri
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001600487 |
Resumo: | ABSTRACT: Asian citrus psyllid, Diaphorina citri, is considered the most important citrus pest worldwide, as it transmits Huanglongbing – serious citrus disease. New efficient and sustainable strategies to control this pest have been investigated and the use of entomopathogenic fungi has become a promising alternative. The objective of this study was to evaluate the effects of adjuvants a) Tween 80 at 0.01 % (v/v); b) Silwet L77 at 0.025 % (v/v) and c) KBRAdj at 0.075 % (v/v) on adhesion, germination and pathogenicity of Isaria fumosorosea ESALQ-1296(5 × 106 conidia mL–1). Female adults of D. citri used in this experiment were sprayed on Citrus limonia seedlings. The sprayed insects were analyzed through scanning electron microscopy (SEM) to identify the most susceptible integument regions for fungus attachment and the effect of adjuvants used. In the pathogenicity test, adjuvants Silwet L77 and KBRAdj presented a higher efficiency than Tween 80. Fungi adhered predominantly to the ventral posterior (abdomen) region in comparison with the dorsal anterior (thorax) region. In addition, adjuvants Silwet L77 and KBRAdj presented faster germination (< 48 h) of I. fumosorosea spores when compared to Tween 80 (> 72 h). Conidial germination in the dorsal part of the thorax of the insects was observed only with adjuvant KBRAdj 72 h post inoculation. |
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Interactions of adjuvants on adhesion and germination of Isaria fumosorosea on adults of Diaphorina citribiological controlAsian citrus psyllidentomopathogenic fungiformulationintegumentABSTRACT: Asian citrus psyllid, Diaphorina citri, is considered the most important citrus pest worldwide, as it transmits Huanglongbing – serious citrus disease. New efficient and sustainable strategies to control this pest have been investigated and the use of entomopathogenic fungi has become a promising alternative. The objective of this study was to evaluate the effects of adjuvants a) Tween 80 at 0.01 % (v/v); b) Silwet L77 at 0.025 % (v/v) and c) KBRAdj at 0.075 % (v/v) on adhesion, germination and pathogenicity of Isaria fumosorosea ESALQ-1296(5 × 106 conidia mL–1). Female adults of D. citri used in this experiment were sprayed on Citrus limonia seedlings. The sprayed insects were analyzed through scanning electron microscopy (SEM) to identify the most susceptible integument regions for fungus attachment and the effect of adjuvants used. In the pathogenicity test, adjuvants Silwet L77 and KBRAdj presented a higher efficiency than Tween 80. Fungi adhered predominantly to the ventral posterior (abdomen) region in comparison with the dorsal anterior (thorax) region. In addition, adjuvants Silwet L77 and KBRAdj presented faster germination (< 48 h) of I. fumosorosea spores when compared to Tween 80 (> 72 h). Conidial germination in the dorsal part of the thorax of the insects was observed only with adjuvant KBRAdj 72 h post inoculation.Escola Superior de Agricultura "Luiz de Queiroz"2019-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001600487Scientia Agricola v.76 n.6 2019reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-992x-2017-0240info:eu-repo/semantics/openAccessArnosti,AndréDelalibera Junior,ItaloConceschi,Marcos RobertoD’Alessandro,Celeste PaolaTravaglini,Raphael VacchiCamargo-Mathias,Maria Izabeleng2019-05-28T00:00:00Zoai:scielo:S0103-90162019001600487Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2019-05-28T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Interactions of adjuvants on adhesion and germination of Isaria fumosorosea on adults of Diaphorina citri |
title |
Interactions of adjuvants on adhesion and germination of Isaria fumosorosea on adults of Diaphorina citri |
spellingShingle |
Interactions of adjuvants on adhesion and germination of Isaria fumosorosea on adults of Diaphorina citri Arnosti,André biological control Asian citrus psyllid entomopathogenic fungi formulation integument |
title_short |
Interactions of adjuvants on adhesion and germination of Isaria fumosorosea on adults of Diaphorina citri |
title_full |
Interactions of adjuvants on adhesion and germination of Isaria fumosorosea on adults of Diaphorina citri |
title_fullStr |
Interactions of adjuvants on adhesion and germination of Isaria fumosorosea on adults of Diaphorina citri |
title_full_unstemmed |
Interactions of adjuvants on adhesion and germination of Isaria fumosorosea on adults of Diaphorina citri |
title_sort |
Interactions of adjuvants on adhesion and germination of Isaria fumosorosea on adults of Diaphorina citri |
author |
Arnosti,André |
author_facet |
Arnosti,André Delalibera Junior,Italo Conceschi,Marcos Roberto D’Alessandro,Celeste Paola Travaglini,Raphael Vacchi Camargo-Mathias,Maria Izabel |
author_role |
author |
author2 |
Delalibera Junior,Italo Conceschi,Marcos Roberto D’Alessandro,Celeste Paola Travaglini,Raphael Vacchi Camargo-Mathias,Maria Izabel |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Arnosti,André Delalibera Junior,Italo Conceschi,Marcos Roberto D’Alessandro,Celeste Paola Travaglini,Raphael Vacchi Camargo-Mathias,Maria Izabel |
dc.subject.por.fl_str_mv |
biological control Asian citrus psyllid entomopathogenic fungi formulation integument |
topic |
biological control Asian citrus psyllid entomopathogenic fungi formulation integument |
description |
ABSTRACT: Asian citrus psyllid, Diaphorina citri, is considered the most important citrus pest worldwide, as it transmits Huanglongbing – serious citrus disease. New efficient and sustainable strategies to control this pest have been investigated and the use of entomopathogenic fungi has become a promising alternative. The objective of this study was to evaluate the effects of adjuvants a) Tween 80 at 0.01 % (v/v); b) Silwet L77 at 0.025 % (v/v) and c) KBRAdj at 0.075 % (v/v) on adhesion, germination and pathogenicity of Isaria fumosorosea ESALQ-1296(5 × 106 conidia mL–1). Female adults of D. citri used in this experiment were sprayed on Citrus limonia seedlings. The sprayed insects were analyzed through scanning electron microscopy (SEM) to identify the most susceptible integument regions for fungus attachment and the effect of adjuvants used. In the pathogenicity test, adjuvants Silwet L77 and KBRAdj presented a higher efficiency than Tween 80. Fungi adhered predominantly to the ventral posterior (abdomen) region in comparison with the dorsal anterior (thorax) region. In addition, adjuvants Silwet L77 and KBRAdj presented faster germination (< 48 h) of I. fumosorosea spores when compared to Tween 80 (> 72 h). Conidial germination in the dorsal part of the thorax of the insects was observed only with adjuvant KBRAdj 72 h post inoculation. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001600487 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001600487 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-992x-2017-0240 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.76 n.6 2019 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936465166893056 |