Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures

Detalhes bibliográficos
Autor(a) principal: Nascimento,Valkirea Matos
Data de Publicação: 2016
Outros Autores: Silva,Luiziana Ferreira, Gomez,José Gregório Cabrera, Fonseca,Gustavo Graciano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000500429
Resumo: ABSTRACT Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no differences in growth rates (μmax) in sucrose and glucose. The growth conditions studied were not favorable to glycerol consumption due to limitations in the energy supply from glycerol.
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spelling Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperaturespolyhydroxyalkanoates (PHAs)metabolismkineticsABSTRACT Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no differences in growth rates (μmax) in sucrose and glucose. The growth conditions studied were not favorable to glycerol consumption due to limitations in the energy supply from glycerol.Escola Superior de Agricultura "Luiz de Queiroz"2016-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000500429Scientia Agricola v.73 n.5 2016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/0103-9016-2015-0196info:eu-repo/semantics/openAccessNascimento,Valkirea MatosSilva,Luiziana FerreiraGomez,José Gregório CabreraFonseca,Gustavo Gracianoeng2016-08-16T00:00:00Zoai:scielo:S0103-90162016000500429Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2016-08-16T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
title Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
spellingShingle Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
Nascimento,Valkirea Matos
polyhydroxyalkanoates (PHAs)
metabolism
kinetics
title_short Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
title_full Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
title_fullStr Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
title_full_unstemmed Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
title_sort Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
author Nascimento,Valkirea Matos
author_facet Nascimento,Valkirea Matos
Silva,Luiziana Ferreira
Gomez,José Gregório Cabrera
Fonseca,Gustavo Graciano
author_role author
author2 Silva,Luiziana Ferreira
Gomez,José Gregório Cabrera
Fonseca,Gustavo Graciano
author2_role author
author
author
dc.contributor.author.fl_str_mv Nascimento,Valkirea Matos
Silva,Luiziana Ferreira
Gomez,José Gregório Cabrera
Fonseca,Gustavo Graciano
dc.subject.por.fl_str_mv polyhydroxyalkanoates (PHAs)
metabolism
kinetics
topic polyhydroxyalkanoates (PHAs)
metabolism
kinetics
description ABSTRACT Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no differences in growth rates (μmax) in sucrose and glucose. The growth conditions studied were not favorable to glycerol consumption due to limitations in the energy supply from glycerol.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000500429
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000500429
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-9016-2015-0196
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.73 n.5 2016
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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