Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures

Detalhes bibliográficos
Autor(a) principal: Nascimento, Valkirea Matos
Data de Publicação: 2016
Outros Autores: Silva, Luiziana Ferreira, Gomez, José Gregório Cabrera, Fonseca, Gustavo Graciano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: https://www.revistas.usp.br/sa/article/view/119295
Resumo: Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no differences in growth rates (μmax) in sucrose and glucose. The growth conditions studied were not favorable to glycerol consumption due to limitations in the energy supply from glycerol.
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spelling Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no differences in growth rates (μmax) in sucrose and glucose. The growth conditions studied were not favorable to glycerol consumption due to limitations in the energy supply from glycerol. Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz2016-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/11929510.1590/0103-9016-2015-0196Scientia Agricola; v. 73 n. 5 (2016); 429-433Scientia Agricola; Vol. 73 Núm. 5 (2016); 429-433Scientia Agricola; Vol. 73 No. 5 (2016); 429-4331678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/sa/article/view/119295/116672Copyright (c) 2016 Scientia Agricolainfo:eu-repo/semantics/openAccessNascimento, Valkirea MatosSilva, Luiziana FerreiraGomez, José Gregório CabreraFonseca, Gustavo Graciano2016-08-18T15:46:21Zoai:revistas.usp.br:article/119295Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2016-08-18T15:46:21Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
title Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
spellingShingle Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
Nascimento, Valkirea Matos
title_short Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
title_full Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
title_fullStr Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
title_full_unstemmed Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
title_sort Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
author Nascimento, Valkirea Matos
author_facet Nascimento, Valkirea Matos
Silva, Luiziana Ferreira
Gomez, José Gregório Cabrera
Fonseca, Gustavo Graciano
author_role author
author2 Silva, Luiziana Ferreira
Gomez, José Gregório Cabrera
Fonseca, Gustavo Graciano
author2_role author
author
author
dc.contributor.author.fl_str_mv Nascimento, Valkirea Matos
Silva, Luiziana Ferreira
Gomez, José Gregório Cabrera
Fonseca, Gustavo Graciano
description Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no differences in growth rates (μmax) in sucrose and glucose. The growth conditions studied were not favorable to glycerol consumption due to limitations in the energy supply from glycerol.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/sa/article/view/119295
10.1590/0103-9016-2015-0196
url https://www.revistas.usp.br/sa/article/view/119295
identifier_str_mv 10.1590/0103-9016-2015-0196
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/sa/article/view/119295/116672
dc.rights.driver.fl_str_mv Copyright (c) 2016 Scientia Agricola
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2016 Scientia Agricola
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
publisher.none.fl_str_mv Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
dc.source.none.fl_str_mv Scientia Agricola; v. 73 n. 5 (2016); 429-433
Scientia Agricola; Vol. 73 Núm. 5 (2016); 429-433
Scientia Agricola; Vol. 73 No. 5 (2016); 429-433
1678-992X
0103-9016
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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