Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | https://www.revistas.usp.br/sa/article/view/119295 |
Resumo: | Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no differences in growth rates (μmax) in sucrose and glucose. The growth conditions studied were not favorable to glycerol consumption due to limitations in the energy supply from glycerol. |
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Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no differences in growth rates (μmax) in sucrose and glucose. The growth conditions studied were not favorable to glycerol consumption due to limitations in the energy supply from glycerol. Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz2016-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/11929510.1590/0103-9016-2015-0196Scientia Agricola; v. 73 n. 5 (2016); 429-433Scientia Agricola; Vol. 73 Núm. 5 (2016); 429-433Scientia Agricola; Vol. 73 No. 5 (2016); 429-4331678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/sa/article/view/119295/116672Copyright (c) 2016 Scientia Agricolainfo:eu-repo/semantics/openAccessNascimento, Valkirea MatosSilva, Luiziana FerreiraGomez, José Gregório CabreraFonseca, Gustavo Graciano2016-08-18T15:46:21Zoai:revistas.usp.br:article/119295Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2016-08-18T15:46:21Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures |
title |
Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures |
spellingShingle |
Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures Nascimento, Valkirea Matos |
title_short |
Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures |
title_full |
Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures |
title_fullStr |
Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures |
title_full_unstemmed |
Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures |
title_sort |
Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures |
author |
Nascimento, Valkirea Matos |
author_facet |
Nascimento, Valkirea Matos Silva, Luiziana Ferreira Gomez, José Gregório Cabrera Fonseca, Gustavo Graciano |
author_role |
author |
author2 |
Silva, Luiziana Ferreira Gomez, José Gregório Cabrera Fonseca, Gustavo Graciano |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Nascimento, Valkirea Matos Silva, Luiziana Ferreira Gomez, José Gregório Cabrera Fonseca, Gustavo Graciano |
description |
Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no differences in growth rates (μmax) in sucrose and glucose. The growth conditions studied were not favorable to glycerol consumption due to limitations in the energy supply from glycerol. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/119295 10.1590/0103-9016-2015-0196 |
url |
https://www.revistas.usp.br/sa/article/view/119295 |
identifier_str_mv |
10.1590/0103-9016-2015-0196 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/119295/116672 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 Scientia Agricola info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 Scientia Agricola |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
dc.source.none.fl_str_mv |
Scientia Agricola; v. 73 n. 5 (2016); 429-433 Scientia Agricola; Vol. 73 Núm. 5 (2016); 429-433 Scientia Agricola; Vol. 73 No. 5 (2016); 429-433 1678-992X 0103-9016 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1800222792878129152 |