Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chicken
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500002 |
Resumo: | Acute heat stress (AHS) modifies the structure of myofibrils affecting functional properties of meat, mainly the water holding capacity. This experiment aimed to identify changes in proteolysis and migration between the myofibrillar and sarcoplasmatic fractions due to pre-slaughter AHS. Myofibrillar fragmentation index (MFI), SDS-PAGE, western blot of vinculin (WB) and shear force (SF) were determined. Six hundred broilers (Gallus gallus) were slaughtered in three different days (ST). In each ST, groups of ten animals were placed in transport crates and submitted to AHS (35ºC, 75 - 85% RH) for 2 hours. Simultaneously, the non-stressed broilers (NS) were kept in thermoneutral environment (22ºC, 83 ± 6.6% RH) within the crates in the same density. After slaughter, the breast muscles were kept refrigerated until the withdrawal of all samples (0, 1, 2, 6 and 24 hours after slaughter). Sampling within AHS and NS birds was collected according to lightness value (normal L* < 49, and high > 51), except for determination of MFI and SF. The lightness was used later to perform SDS-PAGE and WB analyses. MFI kinetics showed that the fragmentation rate was superior in animals NS, indicating that AHS can harm proteolysis and rate of myofibrillar fragmentation. However, the extent of fragmentation did not change, as well as SF values. SDS-PAGE for Troponin fragments indicated a differentiated pattern between AHS and NS. The WB did not show alterations in vinculin fragmentation. Modifications in sarcoplasmatic fraction are observed in meat with high L*values, independent of environmental condition. |
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Scientia Agrícola (Online) |
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Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chickenbroilerheat stresslightnessfragmentation indexshear forceAcute heat stress (AHS) modifies the structure of myofibrils affecting functional properties of meat, mainly the water holding capacity. This experiment aimed to identify changes in proteolysis and migration between the myofibrillar and sarcoplasmatic fractions due to pre-slaughter AHS. Myofibrillar fragmentation index (MFI), SDS-PAGE, western blot of vinculin (WB) and shear force (SF) were determined. Six hundred broilers (Gallus gallus) were slaughtered in three different days (ST). In each ST, groups of ten animals were placed in transport crates and submitted to AHS (35ºC, 75 - 85% RH) for 2 hours. Simultaneously, the non-stressed broilers (NS) were kept in thermoneutral environment (22ºC, 83 ± 6.6% RH) within the crates in the same density. After slaughter, the breast muscles were kept refrigerated until the withdrawal of all samples (0, 1, 2, 6 and 24 hours after slaughter). Sampling within AHS and NS birds was collected according to lightness value (normal L* < 49, and high > 51), except for determination of MFI and SF. The lightness was used later to perform SDS-PAGE and WB analyses. MFI kinetics showed that the fragmentation rate was superior in animals NS, indicating that AHS can harm proteolysis and rate of myofibrillar fragmentation. However, the extent of fragmentation did not change, as well as SF values. SDS-PAGE for Troponin fragments indicated a differentiated pattern between AHS and NS. The WB did not show alterations in vinculin fragmentation. Modifications in sarcoplasmatic fraction are observed in meat with high L*values, independent of environmental condition.Escola Superior de Agricultura "Luiz de Queiroz"2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500002Scientia Agricola v.65 n.5 2008reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162008000500002info:eu-repo/semantics/openAccessSantos,Carolina de CastroDelgado,Eduardo FrancisquineMenten,José Fernando MachadoPedreira,Aparecida Carla de MouraCastillo,Carmen Josefina ContrerasMourão,Gérson BarretoBrossi,CamilaSilva,Iran José Oliveira daeng2008-09-16T00:00:00Zoai:scielo:S0103-90162008000500002Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2008-09-16T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chicken |
title |
Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chicken |
spellingShingle |
Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chicken Santos,Carolina de Castro broiler heat stress lightness fragmentation index shear force |
title_short |
Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chicken |
title_full |
Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chicken |
title_fullStr |
Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chicken |
title_full_unstemmed |
Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chicken |
title_sort |
Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chicken |
author |
Santos,Carolina de Castro |
author_facet |
Santos,Carolina de Castro Delgado,Eduardo Francisquine Menten,José Fernando Machado Pedreira,Aparecida Carla de Moura Castillo,Carmen Josefina Contreras Mourão,Gérson Barreto Brossi,Camila Silva,Iran José Oliveira da |
author_role |
author |
author2 |
Delgado,Eduardo Francisquine Menten,José Fernando Machado Pedreira,Aparecida Carla de Moura Castillo,Carmen Josefina Contreras Mourão,Gérson Barreto Brossi,Camila Silva,Iran José Oliveira da |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos,Carolina de Castro Delgado,Eduardo Francisquine Menten,José Fernando Machado Pedreira,Aparecida Carla de Moura Castillo,Carmen Josefina Contreras Mourão,Gérson Barreto Brossi,Camila Silva,Iran José Oliveira da |
dc.subject.por.fl_str_mv |
broiler heat stress lightness fragmentation index shear force |
topic |
broiler heat stress lightness fragmentation index shear force |
description |
Acute heat stress (AHS) modifies the structure of myofibrils affecting functional properties of meat, mainly the water holding capacity. This experiment aimed to identify changes in proteolysis and migration between the myofibrillar and sarcoplasmatic fractions due to pre-slaughter AHS. Myofibrillar fragmentation index (MFI), SDS-PAGE, western blot of vinculin (WB) and shear force (SF) were determined. Six hundred broilers (Gallus gallus) were slaughtered in three different days (ST). In each ST, groups of ten animals were placed in transport crates and submitted to AHS (35ºC, 75 - 85% RH) for 2 hours. Simultaneously, the non-stressed broilers (NS) were kept in thermoneutral environment (22ºC, 83 ± 6.6% RH) within the crates in the same density. After slaughter, the breast muscles were kept refrigerated until the withdrawal of all samples (0, 1, 2, 6 and 24 hours after slaughter). Sampling within AHS and NS birds was collected according to lightness value (normal L* < 49, and high > 51), except for determination of MFI and SF. The lightness was used later to perform SDS-PAGE and WB analyses. MFI kinetics showed that the fragmentation rate was superior in animals NS, indicating that AHS can harm proteolysis and rate of myofibrillar fragmentation. However, the extent of fragmentation did not change, as well as SF values. SDS-PAGE for Troponin fragments indicated a differentiated pattern between AHS and NS. The WB did not show alterations in vinculin fragmentation. Modifications in sarcoplasmatic fraction are observed in meat with high L*values, independent of environmental condition. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90162008000500002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.65 n.5 2008 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936461020823552 |