Chlorophyll fluorescence of the testa of Brassica oleracea seeds as an indicator of seed maturity and seed quality
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000500016 |
Resumo: | Chlorophyll fluorescence of the testa of seeds is proposed as a non-invasive method for the determination of maturity and quality of seeds. In this study cabbage seeds (Brassica oleracea) were sorted individually based on the chlorophyll fluorescence signals into four subsamples labeled with respect to their chlorophyll fluorescence signal (low, medium, high and very high). The results show that the magnitude of the chlorophyll fluorescence signal was inversely related to the quality of the seeds, expressed as germination %, normal seedling %, germination rate (T50) and uniformity of germination (T75-T25). The seed lot could be improved from 90 to 97% normal seedlings by sorting out 13% of the seeds with very high chlorophyll fluorescence signals. Advantages of the chlorophyll fluorescence method for sorting seeds are the high sensitivity, the method being fully non-destructive, the high speed at which the fluorescence is generated and measured and the specificity for only chlorophyll. Other pigments or substances which can influence seed colour but do not fluoresce at the specific wavelengths of excitation and emission of chlorophyll, will not contribute to the fluorescence signal. These characteristics make chlorophyll fluorescence highly suitable as a new sorting technique. |
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Scientia Agrícola (Online) |
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Chlorophyll fluorescence of the testa of Brassica oleracea seeds as an indicator of seed maturity and seed qualitymaturationchlorophyll fluorescencecabbage seedsortingqualityChlorophyll fluorescence of the testa of seeds is proposed as a non-invasive method for the determination of maturity and quality of seeds. In this study cabbage seeds (Brassica oleracea) were sorted individually based on the chlorophyll fluorescence signals into four subsamples labeled with respect to their chlorophyll fluorescence signal (low, medium, high and very high). The results show that the magnitude of the chlorophyll fluorescence signal was inversely related to the quality of the seeds, expressed as germination %, normal seedling %, germination rate (T50) and uniformity of germination (T75-T25). The seed lot could be improved from 90 to 97% normal seedlings by sorting out 13% of the seeds with very high chlorophyll fluorescence signals. Advantages of the chlorophyll fluorescence method for sorting seeds are the high sensitivity, the method being fully non-destructive, the high speed at which the fluorescence is generated and measured and the specificity for only chlorophyll. Other pigments or substances which can influence seed colour but do not fluoresce at the specific wavelengths of excitation and emission of chlorophyll, will not contribute to the fluorescence signal. These characteristics make chlorophyll fluorescence highly suitable as a new sorting technique.Escola Superior de Agricultura "Luiz de Queiroz"1998-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000500016Scientia Agricola v.55 n.spe 1998reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90161998000500016info:eu-repo/semantics/openAccessJalink,H.Frandas,A.Schoor,R. van derBino,J.B.eng2001-12-11T00:00:00Zoai:scielo:S0103-90161998000500016Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2001-12-11T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Chlorophyll fluorescence of the testa of Brassica oleracea seeds as an indicator of seed maturity and seed quality |
title |
Chlorophyll fluorescence of the testa of Brassica oleracea seeds as an indicator of seed maturity and seed quality |
spellingShingle |
Chlorophyll fluorescence of the testa of Brassica oleracea seeds as an indicator of seed maturity and seed quality Jalink,H. maturation chlorophyll fluorescence cabbage seed sorting quality |
title_short |
Chlorophyll fluorescence of the testa of Brassica oleracea seeds as an indicator of seed maturity and seed quality |
title_full |
Chlorophyll fluorescence of the testa of Brassica oleracea seeds as an indicator of seed maturity and seed quality |
title_fullStr |
Chlorophyll fluorescence of the testa of Brassica oleracea seeds as an indicator of seed maturity and seed quality |
title_full_unstemmed |
Chlorophyll fluorescence of the testa of Brassica oleracea seeds as an indicator of seed maturity and seed quality |
title_sort |
Chlorophyll fluorescence of the testa of Brassica oleracea seeds as an indicator of seed maturity and seed quality |
author |
Jalink,H. |
author_facet |
Jalink,H. Frandas,A. Schoor,R. van der Bino,J.B. |
author_role |
author |
author2 |
Frandas,A. Schoor,R. van der Bino,J.B. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Jalink,H. Frandas,A. Schoor,R. van der Bino,J.B. |
dc.subject.por.fl_str_mv |
maturation chlorophyll fluorescence cabbage seed sorting quality |
topic |
maturation chlorophyll fluorescence cabbage seed sorting quality |
description |
Chlorophyll fluorescence of the testa of seeds is proposed as a non-invasive method for the determination of maturity and quality of seeds. In this study cabbage seeds (Brassica oleracea) were sorted individually based on the chlorophyll fluorescence signals into four subsamples labeled with respect to their chlorophyll fluorescence signal (low, medium, high and very high). The results show that the magnitude of the chlorophyll fluorescence signal was inversely related to the quality of the seeds, expressed as germination %, normal seedling %, germination rate (T50) and uniformity of germination (T75-T25). The seed lot could be improved from 90 to 97% normal seedlings by sorting out 13% of the seeds with very high chlorophyll fluorescence signals. Advantages of the chlorophyll fluorescence method for sorting seeds are the high sensitivity, the method being fully non-destructive, the high speed at which the fluorescence is generated and measured and the specificity for only chlorophyll. Other pigments or substances which can influence seed colour but do not fluoresce at the specific wavelengths of excitation and emission of chlorophyll, will not contribute to the fluorescence signal. These characteristics make chlorophyll fluorescence highly suitable as a new sorting technique. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000500016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000500016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90161998000500016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.55 n.spe 1998 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936456173256704 |