Quantification of chlorophyll fluorescence in soybean seeds by multispectral images and their relationship with physiological potential
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of Seed Science |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372022000100122 |
Resumo: | Abstract: The multispectral image analysis technique to detect chlorophyll fluorescence (CF) in soybean seeds was studied to assess the relationship between CF signals and seed physiological potential. Eight treatments, corresponding to 0%, 2%, 4%, 6%, 8%, 10%, 12%, and 14% green seeds, were used on two cultivars, BMX Desafio RR 8473 RSF and 96R10 IPRO, which passed through different seed quality tests. Initially, the CF of the seeds was determined using 660 nm and 730 nm spectra, and then the germination, electrical conductivity, accelerated aging with saturated NaCl solution, tetrazolium, and computerized seedling image analysis (Vigor-S) tests were performed on the same seeds. A completely randomized design was used, as well as replications of each treatment. Analysis of variance (ANOVA) was performed on the data from germination, vigor, and CF tests using the R® software, and the means were grouped by the Scott-Knott test (p ≤ 0.05). Pearson’s linear correlation coefficients (r) were calculated for all combinations among the evaluations with significance of the r values determined by the t-test (p ≤ 0.05), and multivariate analysis of the principal components was performed. Proportional increases in green seeds contribute to an increase in chlorophyll fluorescence signals and have a negative correlation with seed physiological quality; levels above 4% green seeds in the samples result in marked losses in physiological potential. Therefore, the chlorophyll fluorescence detected through multispectral images is inversely related to the physiological potential of soybean seeds. |
id |
ABRATES-1_5c6302c792874a5386c368f1c378f2d4 |
---|---|
oai_identifier_str |
oai:scielo:S2317-15372022000100122 |
network_acronym_str |
ABRATES-1 |
network_name_str |
Journal of Seed Science |
repository_id_str |
|
spelling |
Quantification of chlorophyll fluorescence in soybean seeds by multispectral images and their relationship with physiological potentialchlorophyll retentionGlycine max (L.) Merrillgreen seedsseed vigorspectroscopyAbstract: The multispectral image analysis technique to detect chlorophyll fluorescence (CF) in soybean seeds was studied to assess the relationship between CF signals and seed physiological potential. Eight treatments, corresponding to 0%, 2%, 4%, 6%, 8%, 10%, 12%, and 14% green seeds, were used on two cultivars, BMX Desafio RR 8473 RSF and 96R10 IPRO, which passed through different seed quality tests. Initially, the CF of the seeds was determined using 660 nm and 730 nm spectra, and then the germination, electrical conductivity, accelerated aging with saturated NaCl solution, tetrazolium, and computerized seedling image analysis (Vigor-S) tests were performed on the same seeds. A completely randomized design was used, as well as replications of each treatment. Analysis of variance (ANOVA) was performed on the data from germination, vigor, and CF tests using the R® software, and the means were grouped by the Scott-Knott test (p ≤ 0.05). Pearson’s linear correlation coefficients (r) were calculated for all combinations among the evaluations with significance of the r values determined by the t-test (p ≤ 0.05), and multivariate analysis of the principal components was performed. Proportional increases in green seeds contribute to an increase in chlorophyll fluorescence signals and have a negative correlation with seed physiological quality; levels above 4% green seeds in the samples result in marked losses in physiological potential. Therefore, the chlorophyll fluorescence detected through multispectral images is inversely related to the physiological potential of soybean seeds.ABRATES - Associação Brasileira de Tecnologia de Sementes2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372022000100122Journal of Seed Science v.44 2022reponame:Journal of Seed Scienceinstname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABRATES10.1590/2317-1545v44258703info:eu-repo/semantics/openAccessFrança-Silva,FabianoCicero,Silvio MoureGomes-Junior,Francisco GuilhienMedeiros,André DantasFrança-Neto,José de BarrosDias,Denise Cunha Fernandes Santoseng2022-07-05T00:00:00Zoai:scielo:S2317-15372022000100122Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2317-1537&lng=en&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br2317-15452317-1537opendoar:2022-07-05T00:00Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)false |
dc.title.none.fl_str_mv |
Quantification of chlorophyll fluorescence in soybean seeds by multispectral images and their relationship with physiological potential |
title |
Quantification of chlorophyll fluorescence in soybean seeds by multispectral images and their relationship with physiological potential |
spellingShingle |
Quantification of chlorophyll fluorescence in soybean seeds by multispectral images and their relationship with physiological potential França-Silva,Fabiano chlorophyll retention Glycine max (L.) Merrill green seeds seed vigor spectroscopy |
title_short |
Quantification of chlorophyll fluorescence in soybean seeds by multispectral images and their relationship with physiological potential |
title_full |
Quantification of chlorophyll fluorescence in soybean seeds by multispectral images and their relationship with physiological potential |
title_fullStr |
Quantification of chlorophyll fluorescence in soybean seeds by multispectral images and their relationship with physiological potential |
title_full_unstemmed |
Quantification of chlorophyll fluorescence in soybean seeds by multispectral images and their relationship with physiological potential |
title_sort |
Quantification of chlorophyll fluorescence in soybean seeds by multispectral images and their relationship with physiological potential |
author |
França-Silva,Fabiano |
author_facet |
França-Silva,Fabiano Cicero,Silvio Moure Gomes-Junior,Francisco Guilhien Medeiros,André Dantas França-Neto,José de Barros Dias,Denise Cunha Fernandes Santos |
author_role |
author |
author2 |
Cicero,Silvio Moure Gomes-Junior,Francisco Guilhien Medeiros,André Dantas França-Neto,José de Barros Dias,Denise Cunha Fernandes Santos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
França-Silva,Fabiano Cicero,Silvio Moure Gomes-Junior,Francisco Guilhien Medeiros,André Dantas França-Neto,José de Barros Dias,Denise Cunha Fernandes Santos |
dc.subject.por.fl_str_mv |
chlorophyll retention Glycine max (L.) Merrill green seeds seed vigor spectroscopy |
topic |
chlorophyll retention Glycine max (L.) Merrill green seeds seed vigor spectroscopy |
description |
Abstract: The multispectral image analysis technique to detect chlorophyll fluorescence (CF) in soybean seeds was studied to assess the relationship between CF signals and seed physiological potential. Eight treatments, corresponding to 0%, 2%, 4%, 6%, 8%, 10%, 12%, and 14% green seeds, were used on two cultivars, BMX Desafio RR 8473 RSF and 96R10 IPRO, which passed through different seed quality tests. Initially, the CF of the seeds was determined using 660 nm and 730 nm spectra, and then the germination, electrical conductivity, accelerated aging with saturated NaCl solution, tetrazolium, and computerized seedling image analysis (Vigor-S) tests were performed on the same seeds. A completely randomized design was used, as well as replications of each treatment. Analysis of variance (ANOVA) was performed on the data from germination, vigor, and CF tests using the R® software, and the means were grouped by the Scott-Knott test (p ≤ 0.05). Pearson’s linear correlation coefficients (r) were calculated for all combinations among the evaluations with significance of the r values determined by the t-test (p ≤ 0.05), and multivariate analysis of the principal components was performed. Proportional increases in green seeds contribute to an increase in chlorophyll fluorescence signals and have a negative correlation with seed physiological quality; levels above 4% green seeds in the samples result in marked losses in physiological potential. Therefore, the chlorophyll fluorescence detected through multispectral images is inversely related to the physiological potential of soybean seeds. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372022000100122 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372022000100122 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2317-1545v44258703 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
dc.source.none.fl_str_mv |
Journal of Seed Science v.44 2022 reponame:Journal of Seed Science instname:Associação Brasileira de Tecnologia de Sementes (ABRATES) instacron:ABRATES |
instname_str |
Associação Brasileira de Tecnologia de Sementes (ABRATES) |
instacron_str |
ABRATES |
institution |
ABRATES |
reponame_str |
Journal of Seed Science |
collection |
Journal of Seed Science |
repository.name.fl_str_mv |
Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES) |
repository.mail.fl_str_mv |
||abrates@abrates.org.br |
_version_ |
1754212983352852480 |