Determination of aspartate kinase activity in maize tissues

Detalhes bibliográficos
Autor(a) principal: Ferreira,Renato Rodrigues
Data de Publicação: 2005
Outros Autores: Vendemiatti,Ariane, Gratão,Priscila Lupino, Lea,Peter John, Azevedo,Ricardo Antunes
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200015
Resumo: Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity.
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spelling Determination of aspartate kinase activity in maize tissuesaspartate pathwaymaizelysinethreoninemethionineLysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity.Escola Superior de Agricultura "Luiz de Queiroz"2005-04-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200015Scientia Agricola v.62 n.2 2005reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162005000200015info:eu-repo/semantics/openAccessFerreira,Renato RodriguesVendemiatti,ArianeGratão,Priscila LupinoLea,Peter JohnAzevedo,Ricardo Antuneseng2005-04-08T00:00:00Zoai:scielo:S0103-90162005000200015Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2005-04-08T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Determination of aspartate kinase activity in maize tissues
title Determination of aspartate kinase activity in maize tissues
spellingShingle Determination of aspartate kinase activity in maize tissues
Ferreira,Renato Rodrigues
aspartate pathway
maize
lysine
threonine
methionine
title_short Determination of aspartate kinase activity in maize tissues
title_full Determination of aspartate kinase activity in maize tissues
title_fullStr Determination of aspartate kinase activity in maize tissues
title_full_unstemmed Determination of aspartate kinase activity in maize tissues
title_sort Determination of aspartate kinase activity in maize tissues
author Ferreira,Renato Rodrigues
author_facet Ferreira,Renato Rodrigues
Vendemiatti,Ariane
Gratão,Priscila Lupino
Lea,Peter John
Azevedo,Ricardo Antunes
author_role author
author2 Vendemiatti,Ariane
Gratão,Priscila Lupino
Lea,Peter John
Azevedo,Ricardo Antunes
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ferreira,Renato Rodrigues
Vendemiatti,Ariane
Gratão,Priscila Lupino
Lea,Peter John
Azevedo,Ricardo Antunes
dc.subject.por.fl_str_mv aspartate pathway
maize
lysine
threonine
methionine
topic aspartate pathway
maize
lysine
threonine
methionine
description Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity.
publishDate 2005
dc.date.none.fl_str_mv 2005-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-90162005000200015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.62 n.2 2005
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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