Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis

Detalhes bibliográficos
Autor(a) principal: Monteiro,Mariza
Data de Publicação: 2003
Outros Autores: Appezzato-da-Glória,Beatriz, Valarini,Maria José, Oliveira,Carlos Alberto de, Vieira,Maria Lucia Carneiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400012
Resumo: Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.
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spelling Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesisplant biotechnologyin vitro culturemorphogenesisforage legumeAlfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.Escola Superior de Agricultura "Luiz de Queiroz"2003-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400012Scientia Agricola v.60 n.4 2003reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162003000400012info:eu-repo/semantics/openAccessMonteiro,MarizaAppezzato-da-Glória,BeatrizValarini,Maria JoséOliveira,Carlos Alberto deVieira,Maria Lucia Carneiroeng2003-10-24T00:00:00Zoai:scielo:S0103-90162003000400012Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2003-10-24T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis
title Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis
spellingShingle Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis
Monteiro,Mariza
plant biotechnology
in vitro culture
morphogenesis
forage legume
title_short Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis
title_full Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis
title_fullStr Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis
title_full_unstemmed Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis
title_sort Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis
author Monteiro,Mariza
author_facet Monteiro,Mariza
Appezzato-da-Glória,Beatriz
Valarini,Maria José
Oliveira,Carlos Alberto de
Vieira,Maria Lucia Carneiro
author_role author
author2 Appezzato-da-Glória,Beatriz
Valarini,Maria José
Oliveira,Carlos Alberto de
Vieira,Maria Lucia Carneiro
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Monteiro,Mariza
Appezzato-da-Glória,Beatriz
Valarini,Maria José
Oliveira,Carlos Alberto de
Vieira,Maria Lucia Carneiro
dc.subject.por.fl_str_mv plant biotechnology
in vitro culture
morphogenesis
forage legume
topic plant biotechnology
in vitro culture
morphogenesis
forage legume
description Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.
publishDate 2003
dc.date.none.fl_str_mv 2003-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-90162003000400012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.60 n.4 2003
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
_version_ 1748936459185815552

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