Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2003 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400012 |
Resumo: | Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa. |
id |
USP-18_cb0dc74efac2b7eba2e6395ed9d47209 |
---|---|
oai_identifier_str |
oai:scielo:S0103-90162003000400012 |
network_acronym_str |
USP-18 |
network_name_str |
Scientia Agrícola (Online) |
repository_id_str |
|
spelling |
Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesisplant biotechnologyin vitro culturemorphogenesisforage legumeAlfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.Escola Superior de Agricultura "Luiz de Queiroz"2003-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400012Scientia Agricola v.60 n.4 2003reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162003000400012info:eu-repo/semantics/openAccessMonteiro,MarizaAppezzato-da-Glória,BeatrizValarini,Maria JoséOliveira,Carlos Alberto deVieira,Maria Lucia Carneiroeng2003-10-24T00:00:00Zoai:scielo:S0103-90162003000400012Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2003-10-24T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis |
title |
Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis |
spellingShingle |
Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis Monteiro,Mariza plant biotechnology in vitro culture morphogenesis forage legume |
title_short |
Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis |
title_full |
Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis |
title_fullStr |
Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis |
title_full_unstemmed |
Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis |
title_sort |
Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis |
author |
Monteiro,Mariza |
author_facet |
Monteiro,Mariza Appezzato-da-Glória,Beatriz Valarini,Maria José Oliveira,Carlos Alberto de Vieira,Maria Lucia Carneiro |
author_role |
author |
author2 |
Appezzato-da-Glória,Beatriz Valarini,Maria José Oliveira,Carlos Alberto de Vieira,Maria Lucia Carneiro |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Monteiro,Mariza Appezzato-da-Glória,Beatriz Valarini,Maria José Oliveira,Carlos Alberto de Vieira,Maria Lucia Carneiro |
dc.subject.por.fl_str_mv |
plant biotechnology in vitro culture morphogenesis forage legume |
topic |
plant biotechnology in vitro culture morphogenesis forage legume |
description |
Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90162003000400012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.60 n.4 2003 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936459185815552 |