Histological study of fresh versus frozen semitendinous muscle tendon allografts

Detalhes bibliográficos
Autor(a) principal: Bitar, Alexandre Carneiro
Data de Publicação: 2010
Outros Autores: Santos, Luiz Augusto Ubirajara, Croci, Alberto Tesconi, Pereira, João Alberto Ramos Maradei, França Bisneto, Edgard N., Giovani, Arlete Mazzini Miranda, Oliveira, Claudia Regina G. C. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/18387
Resumo: OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at -80°C in comparison to a control group kept at only -4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures. METHODS: In a tissue bank, 10 semitendinous tendons from 10 cadavers were frozen at -80ºC as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still "fresh". RESULTS: There was no histological difference between the fresh and frozen samples in relation to seven variables. CONCLUSIONS: Semitendinous muscle tendon allografts can be submitted to cryopreservation at -80ºC without suffering histological modifications.
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spelling Histological study of fresh versus frozen semitendinous muscle tendon allografts CryopreservationTissue banksHistologyTendonsTissue transplantation OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at -80°C in comparison to a control group kept at only -4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures. METHODS: In a tissue bank, 10 semitendinous tendons from 10 cadavers were frozen at -80ºC as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still "fresh". RESULTS: There was no histological difference between the fresh and frozen samples in relation to seven variables. CONCLUSIONS: Semitendinous muscle tendon allografts can be submitted to cryopreservation at -80ºC without suffering histological modifications. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1838710.1590/S1807-59322010000300010Clinics; Vol. 65 No. 3 (2010); 297-303 Clinics; v. 65 n. 3 (2010); 297-303 Clinics; Vol. 65 Núm. 3 (2010); 297-303 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/18387/20450Bitar, Alexandre CarneiroSantos, Luiz Augusto UbirajaraCroci, Alberto TesconiPereira, João Alberto Ramos MaradeiFrança Bisneto, Edgard N.Giovani, Arlete Mazzini MirandaOliveira, Claudia Regina G. C. M.info:eu-repo/semantics/openAccess2012-05-23T11:19:19Zoai:revistas.usp.br:article/18387Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-23T11:19:19Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Histological study of fresh versus frozen semitendinous muscle tendon allografts
title Histological study of fresh versus frozen semitendinous muscle tendon allografts
spellingShingle Histological study of fresh versus frozen semitendinous muscle tendon allografts
Bitar, Alexandre Carneiro
Cryopreservation
Tissue banks
Histology
Tendons
Tissue transplantation
title_short Histological study of fresh versus frozen semitendinous muscle tendon allografts
title_full Histological study of fresh versus frozen semitendinous muscle tendon allografts
title_fullStr Histological study of fresh versus frozen semitendinous muscle tendon allografts
title_full_unstemmed Histological study of fresh versus frozen semitendinous muscle tendon allografts
title_sort Histological study of fresh versus frozen semitendinous muscle tendon allografts
author Bitar, Alexandre Carneiro
author_facet Bitar, Alexandre Carneiro
Santos, Luiz Augusto Ubirajara
Croci, Alberto Tesconi
Pereira, João Alberto Ramos Maradei
França Bisneto, Edgard N.
Giovani, Arlete Mazzini Miranda
Oliveira, Claudia Regina G. C. M.
author_role author
author2 Santos, Luiz Augusto Ubirajara
Croci, Alberto Tesconi
Pereira, João Alberto Ramos Maradei
França Bisneto, Edgard N.
Giovani, Arlete Mazzini Miranda
Oliveira, Claudia Regina G. C. M.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bitar, Alexandre Carneiro
Santos, Luiz Augusto Ubirajara
Croci, Alberto Tesconi
Pereira, João Alberto Ramos Maradei
França Bisneto, Edgard N.
Giovani, Arlete Mazzini Miranda
Oliveira, Claudia Regina G. C. M.
dc.subject.por.fl_str_mv Cryopreservation
Tissue banks
Histology
Tendons
Tissue transplantation
topic Cryopreservation
Tissue banks
Histology
Tendons
Tissue transplantation
description OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at -80°C in comparison to a control group kept at only -4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures. METHODS: In a tissue bank, 10 semitendinous tendons from 10 cadavers were frozen at -80ºC as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still "fresh". RESULTS: There was no histological difference between the fresh and frozen samples in relation to seven variables. CONCLUSIONS: Semitendinous muscle tendon allografts can be submitted to cryopreservation at -80ºC without suffering histological modifications.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/18387
10.1590/S1807-59322010000300010
url https://www.revistas.usp.br/clinics/article/view/18387
identifier_str_mv 10.1590/S1807-59322010000300010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/18387/20450
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 65 No. 3 (2010); 297-303
Clinics; v. 65 n. 3 (2010); 297-303
Clinics; Vol. 65 Núm. 3 (2010); 297-303
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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