Standardization of a spinal cord lesion model and neurologic evaluation using mice
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/144532 |
Resumo: | OBJECTIVE: To standardize a spinal cord lesion mouse model. METHODS: Thirty BALB/c mice were divided into five groups: four experimental groups and one control group (sham). The experimental groups were subjected to spinal cord lesion by a weight drop from different heights after laminectomy whereas the sham group only underwent laminectomy. Mice were observed for six weeks, and functional behavior scales were applied. The mice were then euthanized, and histological investigations were performed to confirm and score spinal cord lesion. The findings were evaluated to prove whether the method of administering spinal cord lesion was effective and different among the groups. Additionally, we correlated the results of the functional scales with the results from the histology evaluations to identify which scale is more reliable. RESULTS: One mouse presented autophagia, and six mice died during the experiment. Because four of the mice that died were in Group 5, Group 5 was excluded from the study. All the functional scales assessed proved to be significantly different from each other, and mice presented functional evolution during the experiment. Spinal cord lesion was confirmed by histology, and the results showed a high correlation between the Basso, Beattie, Bresnahan Locomotor Rating Scale and the Basso Mouse Scale. The mouse function scale showed a moderate to high correlation with the histological findings, and the horizontal ladder test had a high correlation with neurologic degeneration but no correlation with the other histological parameters evaluated. CONCLUSION: This spinal cord lesion mouse model proved to be effective and reliable with exception of lesions caused by a 10-g drop from 50 mm, which resulted in unacceptable mortality. The Basso, Beattie, Bresnahan Locomotor Rating Scale and Basso Mouse Scale are the most reliable functional assessments, and but the horizontal ladder test is not recommended. |
id |
USP-19_374cf41bea82a813a73808a17c5407fb |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/144532 |
network_acronym_str |
USP-19 |
network_name_str |
Clinics |
repository_id_str |
|
spelling |
Standardization of a spinal cord lesion model and neurologic evaluation using miceReference StandardsMiceSpinal CordSpinal Cord CompressionOBJECTIVE: To standardize a spinal cord lesion mouse model. METHODS: Thirty BALB/c mice were divided into five groups: four experimental groups and one control group (sham). The experimental groups were subjected to spinal cord lesion by a weight drop from different heights after laminectomy whereas the sham group only underwent laminectomy. Mice were observed for six weeks, and functional behavior scales were applied. The mice were then euthanized, and histological investigations were performed to confirm and score spinal cord lesion. The findings were evaluated to prove whether the method of administering spinal cord lesion was effective and different among the groups. Additionally, we correlated the results of the functional scales with the results from the histology evaluations to identify which scale is more reliable. RESULTS: One mouse presented autophagia, and six mice died during the experiment. Because four of the mice that died were in Group 5, Group 5 was excluded from the study. All the functional scales assessed proved to be significantly different from each other, and mice presented functional evolution during the experiment. Spinal cord lesion was confirmed by histology, and the results showed a high correlation between the Basso, Beattie, Bresnahan Locomotor Rating Scale and the Basso Mouse Scale. The mouse function scale showed a moderate to high correlation with the histological findings, and the horizontal ladder test had a high correlation with neurologic degeneration but no correlation with the other histological parameters evaluated. CONCLUSION: This spinal cord lesion mouse model proved to be effective and reliable with exception of lesions caused by a 10-g drop from 50 mm, which resulted in unacceptable mortality. The Basso, Beattie, Bresnahan Locomotor Rating Scale and Basso Mouse Scale are the most reliable functional assessments, and but the horizontal ladder test is not recommended.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/14453210.6061/clinics/2018/e293Clinics; Vol. 73 (2018); e293Clinics; v. 73 (2018); e293Clinics; Vol. 73 (2018); e2931980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/144532/138859Copyright (c) 2018 Clinicsinfo:eu-repo/semantics/openAccessBorges, Paulo AlvimCristante, Alexandre FogaçaBarros-Filho, Tarcísio Eloy Pessoa deNatalino, Renato Jose MendonçaSantos, Gustavo Bispo dosMarcon, Raphael Marcus2019-05-14T11:48:50Zoai:revistas.usp.br:article/144532Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2019-05-14T11:48:50Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Standardization of a spinal cord lesion model and neurologic evaluation using mice |
title |
Standardization of a spinal cord lesion model and neurologic evaluation using mice |
spellingShingle |
Standardization of a spinal cord lesion model and neurologic evaluation using mice Borges, Paulo Alvim Reference Standards Mice Spinal Cord Spinal Cord Compression |
title_short |
Standardization of a spinal cord lesion model and neurologic evaluation using mice |
title_full |
Standardization of a spinal cord lesion model and neurologic evaluation using mice |
title_fullStr |
Standardization of a spinal cord lesion model and neurologic evaluation using mice |
title_full_unstemmed |
Standardization of a spinal cord lesion model and neurologic evaluation using mice |
title_sort |
Standardization of a spinal cord lesion model and neurologic evaluation using mice |
author |
Borges, Paulo Alvim |
author_facet |
Borges, Paulo Alvim Cristante, Alexandre Fogaça Barros-Filho, Tarcísio Eloy Pessoa de Natalino, Renato Jose Mendonça Santos, Gustavo Bispo dos Marcon, Raphael Marcus |
author_role |
author |
author2 |
Cristante, Alexandre Fogaça Barros-Filho, Tarcísio Eloy Pessoa de Natalino, Renato Jose Mendonça Santos, Gustavo Bispo dos Marcon, Raphael Marcus |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Borges, Paulo Alvim Cristante, Alexandre Fogaça Barros-Filho, Tarcísio Eloy Pessoa de Natalino, Renato Jose Mendonça Santos, Gustavo Bispo dos Marcon, Raphael Marcus |
dc.subject.por.fl_str_mv |
Reference Standards Mice Spinal Cord Spinal Cord Compression |
topic |
Reference Standards Mice Spinal Cord Spinal Cord Compression |
description |
OBJECTIVE: To standardize a spinal cord lesion mouse model. METHODS: Thirty BALB/c mice were divided into five groups: four experimental groups and one control group (sham). The experimental groups were subjected to spinal cord lesion by a weight drop from different heights after laminectomy whereas the sham group only underwent laminectomy. Mice were observed for six weeks, and functional behavior scales were applied. The mice were then euthanized, and histological investigations were performed to confirm and score spinal cord lesion. The findings were evaluated to prove whether the method of administering spinal cord lesion was effective and different among the groups. Additionally, we correlated the results of the functional scales with the results from the histology evaluations to identify which scale is more reliable. RESULTS: One mouse presented autophagia, and six mice died during the experiment. Because four of the mice that died were in Group 5, Group 5 was excluded from the study. All the functional scales assessed proved to be significantly different from each other, and mice presented functional evolution during the experiment. Spinal cord lesion was confirmed by histology, and the results showed a high correlation between the Basso, Beattie, Bresnahan Locomotor Rating Scale and the Basso Mouse Scale. The mouse function scale showed a moderate to high correlation with the histological findings, and the horizontal ladder test had a high correlation with neurologic degeneration but no correlation with the other histological parameters evaluated. CONCLUSION: This spinal cord lesion mouse model proved to be effective and reliable with exception of lesions caused by a 10-g drop from 50 mm, which resulted in unacceptable mortality. The Basso, Beattie, Bresnahan Locomotor Rating Scale and Basso Mouse Scale are the most reliable functional assessments, and but the horizontal ladder test is not recommended. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/144532 10.6061/clinics/2018/e293 |
url |
https://www.revistas.usp.br/clinics/article/view/144532 |
identifier_str_mv |
10.6061/clinics/2018/e293 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/144532/138859 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 73 (2018); e293 Clinics; v. 73 (2018); e293 Clinics; Vol. 73 (2018); e293 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222763660607488 |