Invariant Natural Killer T-cells and their subtypes may play a role in the pathogenesis of endometriosis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/213316 |
Resumo: | Objective: To evaluate the frequencies of iNKT cells and their subsets in patients with deep endometriosis. Methods: A case-control study was conducted between 2013 and 2015, with 73 patients distributed into two groups: 47 women with a histological diagnosis of endometriosis and 26 controls. Peripheral blood, endometriosis lesions, and healthy peritoneal samples were collected on the day of surgery to determine the frequencies of iNKT cells and subtypes via flow cytometry analysis. Results: The authors observed a lower number of iNKT (p = 0.01) and Double-Negative (DN) iNKT cells (p = 0.02) in the blood of patients with endometriosis than in the control group. The number of DN iNKT IL-17+ cells in the secretory phase was lower in the endometriosis group (p = 0.049). There was an increase in the secretion of IL-17 by CD4+ iNKT cells in the blood of patients with endometriosis and severe dysmenorrhea (p = 0.038), and severe acyclic pelvic pain (p = 0.048). Patients with severe dysmenorrhea also had a decreased number of CD4+ CCR7+ cells (p = 0.022). Conclusion: The decreased number of total iNKT and DN iNKT cells in patients with endometriosis suggests that iNKT cells play a role in the pathogenesis of endometriosis and can be used to develop new diagnostic and therapeutic agents. |
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Clinics |
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Invariant Natural Killer T-cells and their subtypes may play a role in the pathogenesis of endometriosisDeep endometriosisInvariant natural Killer T-cellsCytokinesFlow cytometryObjective: To evaluate the frequencies of iNKT cells and their subsets in patients with deep endometriosis. Methods: A case-control study was conducted between 2013 and 2015, with 73 patients distributed into two groups: 47 women with a histological diagnosis of endometriosis and 26 controls. Peripheral blood, endometriosis lesions, and healthy peritoneal samples were collected on the day of surgery to determine the frequencies of iNKT cells and subtypes via flow cytometry analysis. Results: The authors observed a lower number of iNKT (p = 0.01) and Double-Negative (DN) iNKT cells (p = 0.02) in the blood of patients with endometriosis than in the control group. The number of DN iNKT IL-17+ cells in the secretory phase was lower in the endometriosis group (p = 0.049). There was an increase in the secretion of IL-17 by CD4+ iNKT cells in the blood of patients with endometriosis and severe dysmenorrhea (p = 0.038), and severe acyclic pelvic pain (p = 0.048). Patients with severe dysmenorrhea also had a decreased number of CD4+ CCR7+ cells (p = 0.022). Conclusion: The decreased number of total iNKT and DN iNKT cells in patients with endometriosis suggests that iNKT cells play a role in the pathogenesis of endometriosis and can be used to develop new diagnostic and therapeutic agents.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2022-05-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/21331610.1016/j.clinsp.2022.100032Clinics; Vol. 77 (2022); 100032Clinics; v. 77 (2022); 100032Clinics; Vol. 77 (2022); 1000321980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/213316/195265Copyright (c) 2023 Clinicsinfo:eu-repo/semantics/openAccessCorrea, Frederico J.S.Andres, Marina PaulaRocha, Tainá PezzinCarvalho, Ana Eduarda Z.Aloia, Thiago P.A.Corpa, Marcus V.N.Kallas, Esper G.Mangueira, Cristóvão L.P.Baracat, Edmund C.Carvalho, Karina I.Abrão, Mauricio S.2023-07-06T13:04:56Zoai:revistas.usp.br:article/213316Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2023-07-06T13:04:56Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Invariant Natural Killer T-cells and their subtypes may play a role in the pathogenesis of endometriosis |
title |
Invariant Natural Killer T-cells and their subtypes may play a role in the pathogenesis of endometriosis |
spellingShingle |
Invariant Natural Killer T-cells and their subtypes may play a role in the pathogenesis of endometriosis Correa, Frederico J.S. Deep endometriosis Invariant natural Killer T-cells Cytokines Flow cytometry |
title_short |
Invariant Natural Killer T-cells and their subtypes may play a role in the pathogenesis of endometriosis |
title_full |
Invariant Natural Killer T-cells and their subtypes may play a role in the pathogenesis of endometriosis |
title_fullStr |
Invariant Natural Killer T-cells and their subtypes may play a role in the pathogenesis of endometriosis |
title_full_unstemmed |
Invariant Natural Killer T-cells and their subtypes may play a role in the pathogenesis of endometriosis |
title_sort |
Invariant Natural Killer T-cells and their subtypes may play a role in the pathogenesis of endometriosis |
author |
Correa, Frederico J.S. |
author_facet |
Correa, Frederico J.S. Andres, Marina Paula Rocha, Tainá Pezzin Carvalho, Ana Eduarda Z. Aloia, Thiago P.A. Corpa, Marcus V.N. Kallas, Esper G. Mangueira, Cristóvão L.P. Baracat, Edmund C. Carvalho, Karina I. Abrão, Mauricio S. |
author_role |
author |
author2 |
Andres, Marina Paula Rocha, Tainá Pezzin Carvalho, Ana Eduarda Z. Aloia, Thiago P.A. Corpa, Marcus V.N. Kallas, Esper G. Mangueira, Cristóvão L.P. Baracat, Edmund C. Carvalho, Karina I. Abrão, Mauricio S. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Correa, Frederico J.S. Andres, Marina Paula Rocha, Tainá Pezzin Carvalho, Ana Eduarda Z. Aloia, Thiago P.A. Corpa, Marcus V.N. Kallas, Esper G. Mangueira, Cristóvão L.P. Baracat, Edmund C. Carvalho, Karina I. Abrão, Mauricio S. |
dc.subject.por.fl_str_mv |
Deep endometriosis Invariant natural Killer T-cells Cytokines Flow cytometry |
topic |
Deep endometriosis Invariant natural Killer T-cells Cytokines Flow cytometry |
description |
Objective: To evaluate the frequencies of iNKT cells and their subsets in patients with deep endometriosis. Methods: A case-control study was conducted between 2013 and 2015, with 73 patients distributed into two groups: 47 women with a histological diagnosis of endometriosis and 26 controls. Peripheral blood, endometriosis lesions, and healthy peritoneal samples were collected on the day of surgery to determine the frequencies of iNKT cells and subtypes via flow cytometry analysis. Results: The authors observed a lower number of iNKT (p = 0.01) and Double-Negative (DN) iNKT cells (p = 0.02) in the blood of patients with endometriosis than in the control group. The number of DN iNKT IL-17+ cells in the secretory phase was lower in the endometriosis group (p = 0.049). There was an increase in the secretion of IL-17 by CD4+ iNKT cells in the blood of patients with endometriosis and severe dysmenorrhea (p = 0.038), and severe acyclic pelvic pain (p = 0.048). Patients with severe dysmenorrhea also had a decreased number of CD4+ CCR7+ cells (p = 0.022). Conclusion: The decreased number of total iNKT and DN iNKT cells in patients with endometriosis suggests that iNKT cells play a role in the pathogenesis of endometriosis and can be used to develop new diagnostic and therapeutic agents. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/213316 10.1016/j.clinsp.2022.100032 |
url |
https://www.revistas.usp.br/clinics/article/view/213316 |
identifier_str_mv |
10.1016/j.clinsp.2022.100032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/213316/195265 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 77 (2022); 100032 Clinics; v. 77 (2022); 100032 Clinics; Vol. 77 (2022); 100032 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222766601863168 |