Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens

Detalhes bibliográficos
Autor(a) principal: Perniconi, Sergio Eduardo
Data de Publicação: 2008
Outros Autores: Simões, Manuel de Jesus, Simões, Ricardo dos Santos, Haidar, Mauro Abi, Baracat, Edmund C, Soares Jr, Jose Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/17810
Resumo: OBJECTIVE: To evaluate the effect of different concentrations of estrogen on the ovarian superficial epithelium in senile female rats. Design: Fifty female rats at 15 months of age and with irregular estrous cycles were selected and randomly divided into five experimental groups containing equal numbers of animals in each: GPROP, control group receiving vehicle only; GE0.05mg, group receiving conjugated equine estrogens (CEE) at a dose of 50 µg/kg; GE0.5mg, group receiving CEE at 500 µg/kg; GE1mg, group receiving CEE at 1 mg/kg; and GE2mg, receiving CEE at 2 mg/kg. The length of treatment was 21 days. After this period, the animals were anesthetized and the ovaries were fixed in 10% formaldehyde and processed for routine histology. Histomorphology was analyzed by light microscopy, and histomorphometrics were evaluated using the Imagelab program. RESULTS: In the GPROP and GE0.05mg groups, the superficial epithelium of the ovary had a simple cuboidal shape, and as the estrogen dose increased, the epithelium thickened, with pseudo-stratified or stratified epithelium appearing in the GE2mg group. The animals in the group given the highest estrogen dose (GE2mg) showed the thickest ovarian epithelium and the largest perimeter and surface area of the surface ovarian epithelium (P < 0.01). However, the difference in epithelium thickness between the GE0.5mg and GE1mg groups was only slight. CONCLUSION: Our data suggest that CEE at a dose of 2 mg/kg may induce marked proliferation of rat ovarian epithelium.
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spelling Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens EstrogensSuperficial epitheliumOvaryAnimal Model OBJECTIVE: To evaluate the effect of different concentrations of estrogen on the ovarian superficial epithelium in senile female rats. Design: Fifty female rats at 15 months of age and with irregular estrous cycles were selected and randomly divided into five experimental groups containing equal numbers of animals in each: GPROP, control group receiving vehicle only; GE0.05mg, group receiving conjugated equine estrogens (CEE) at a dose of 50 µg/kg; GE0.5mg, group receiving CEE at 500 µg/kg; GE1mg, group receiving CEE at 1 mg/kg; and GE2mg, receiving CEE at 2 mg/kg. The length of treatment was 21 days. After this period, the animals were anesthetized and the ovaries were fixed in 10% formaldehyde and processed for routine histology. Histomorphology was analyzed by light microscopy, and histomorphometrics were evaluated using the Imagelab program. RESULTS: In the GPROP and GE0.05mg groups, the superficial epithelium of the ovary had a simple cuboidal shape, and as the estrogen dose increased, the epithelium thickened, with pseudo-stratified or stratified epithelium appearing in the GE2mg group. The animals in the group given the highest estrogen dose (GE2mg) showed the thickest ovarian epithelium and the largest perimeter and surface area of the surface ovarian epithelium (P < 0.01). However, the difference in epithelium thickness between the GE0.5mg and GE1mg groups was only slight. CONCLUSION: Our data suggest that CEE at a dose of 2 mg/kg may induce marked proliferation of rat ovarian epithelium. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1781010.1590/S1807-59322008000300016Clinics; Vol. 63 No. 3 (2008); 381-388 Clinics; v. 63 n. 3 (2008); 381-388 Clinics; Vol. 63 Núm. 3 (2008); 381-388 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/17810/19875Perniconi, Sergio EduardoSimões, Manuel de JesusSimões, Ricardo dos SantosHaidar, Mauro AbiBaracat, Edmund CSoares Jr, Jose Mariainfo:eu-repo/semantics/openAccess2012-05-22T18:35:23Zoai:revistas.usp.br:article/17810Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-22T18:35:23Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens
title Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens
spellingShingle Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens
Perniconi, Sergio Eduardo
Estrogens
Superficial epithelium
Ovary
Animal Model
title_short Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens
title_full Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens
title_fullStr Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens
title_full_unstemmed Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens
title_sort Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens
author Perniconi, Sergio Eduardo
author_facet Perniconi, Sergio Eduardo
Simões, Manuel de Jesus
Simões, Ricardo dos Santos
Haidar, Mauro Abi
Baracat, Edmund C
Soares Jr, Jose Maria
author_role author
author2 Simões, Manuel de Jesus
Simões, Ricardo dos Santos
Haidar, Mauro Abi
Baracat, Edmund C
Soares Jr, Jose Maria
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Perniconi, Sergio Eduardo
Simões, Manuel de Jesus
Simões, Ricardo dos Santos
Haidar, Mauro Abi
Baracat, Edmund C
Soares Jr, Jose Maria
dc.subject.por.fl_str_mv Estrogens
Superficial epithelium
Ovary
Animal Model
topic Estrogens
Superficial epithelium
Ovary
Animal Model
description OBJECTIVE: To evaluate the effect of different concentrations of estrogen on the ovarian superficial epithelium in senile female rats. Design: Fifty female rats at 15 months of age and with irregular estrous cycles were selected and randomly divided into five experimental groups containing equal numbers of animals in each: GPROP, control group receiving vehicle only; GE0.05mg, group receiving conjugated equine estrogens (CEE) at a dose of 50 µg/kg; GE0.5mg, group receiving CEE at 500 µg/kg; GE1mg, group receiving CEE at 1 mg/kg; and GE2mg, receiving CEE at 2 mg/kg. The length of treatment was 21 days. After this period, the animals were anesthetized and the ovaries were fixed in 10% formaldehyde and processed for routine histology. Histomorphology was analyzed by light microscopy, and histomorphometrics were evaluated using the Imagelab program. RESULTS: In the GPROP and GE0.05mg groups, the superficial epithelium of the ovary had a simple cuboidal shape, and as the estrogen dose increased, the epithelium thickened, with pseudo-stratified or stratified epithelium appearing in the GE2mg group. The animals in the group given the highest estrogen dose (GE2mg) showed the thickest ovarian epithelium and the largest perimeter and surface area of the surface ovarian epithelium (P < 0.01). However, the difference in epithelium thickness between the GE0.5mg and GE1mg groups was only slight. CONCLUSION: Our data suggest that CEE at a dose of 2 mg/kg may induce marked proliferation of rat ovarian epithelium.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/17810
10.1590/S1807-59322008000300016
url https://www.revistas.usp.br/clinics/article/view/17810
identifier_str_mv 10.1590/S1807-59322008000300016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/17810/19875
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 63 No. 3 (2008); 381-388
Clinics; v. 63 n. 3 (2008); 381-388
Clinics; Vol. 63 Núm. 3 (2008); 381-388
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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