Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/17810 |
Resumo: | OBJECTIVE: To evaluate the effect of different concentrations of estrogen on the ovarian superficial epithelium in senile female rats. Design: Fifty female rats at 15 months of age and with irregular estrous cycles were selected and randomly divided into five experimental groups containing equal numbers of animals in each: GPROP, control group receiving vehicle only; GE0.05mg, group receiving conjugated equine estrogens (CEE) at a dose of 50 µg/kg; GE0.5mg, group receiving CEE at 500 µg/kg; GE1mg, group receiving CEE at 1 mg/kg; and GE2mg, receiving CEE at 2 mg/kg. The length of treatment was 21 days. After this period, the animals were anesthetized and the ovaries were fixed in 10% formaldehyde and processed for routine histology. Histomorphology was analyzed by light microscopy, and histomorphometrics were evaluated using the Imagelab program. RESULTS: In the GPROP and GE0.05mg groups, the superficial epithelium of the ovary had a simple cuboidal shape, and as the estrogen dose increased, the epithelium thickened, with pseudo-stratified or stratified epithelium appearing in the GE2mg group. The animals in the group given the highest estrogen dose (GE2mg) showed the thickest ovarian epithelium and the largest perimeter and surface area of the surface ovarian epithelium (P < 0.01). However, the difference in epithelium thickness between the GE0.5mg and GE1mg groups was only slight. CONCLUSION: Our data suggest that CEE at a dose of 2 mg/kg may induce marked proliferation of rat ovarian epithelium. |
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Clinics |
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Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens EstrogensSuperficial epitheliumOvaryAnimal Model OBJECTIVE: To evaluate the effect of different concentrations of estrogen on the ovarian superficial epithelium in senile female rats. Design: Fifty female rats at 15 months of age and with irregular estrous cycles were selected and randomly divided into five experimental groups containing equal numbers of animals in each: GPROP, control group receiving vehicle only; GE0.05mg, group receiving conjugated equine estrogens (CEE) at a dose of 50 µg/kg; GE0.5mg, group receiving CEE at 500 µg/kg; GE1mg, group receiving CEE at 1 mg/kg; and GE2mg, receiving CEE at 2 mg/kg. The length of treatment was 21 days. After this period, the animals were anesthetized and the ovaries were fixed in 10% formaldehyde and processed for routine histology. Histomorphology was analyzed by light microscopy, and histomorphometrics were evaluated using the Imagelab program. RESULTS: In the GPROP and GE0.05mg groups, the superficial epithelium of the ovary had a simple cuboidal shape, and as the estrogen dose increased, the epithelium thickened, with pseudo-stratified or stratified epithelium appearing in the GE2mg group. The animals in the group given the highest estrogen dose (GE2mg) showed the thickest ovarian epithelium and the largest perimeter and surface area of the surface ovarian epithelium (P < 0.01). However, the difference in epithelium thickness between the GE0.5mg and GE1mg groups was only slight. CONCLUSION: Our data suggest that CEE at a dose of 2 mg/kg may induce marked proliferation of rat ovarian epithelium. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1781010.1590/S1807-59322008000300016Clinics; Vol. 63 No. 3 (2008); 381-388 Clinics; v. 63 n. 3 (2008); 381-388 Clinics; Vol. 63 Núm. 3 (2008); 381-388 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/17810/19875Perniconi, Sergio EduardoSimões, Manuel de JesusSimões, Ricardo dos SantosHaidar, Mauro AbiBaracat, Edmund CSoares Jr, Jose Mariainfo:eu-repo/semantics/openAccess2012-05-22T18:35:23Zoai:revistas.usp.br:article/17810Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-22T18:35:23Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens |
title |
Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens |
spellingShingle |
Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens Perniconi, Sergio Eduardo Estrogens Superficial epithelium Ovary Animal Model |
title_short |
Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens |
title_full |
Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens |
title_fullStr |
Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens |
title_full_unstemmed |
Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens |
title_sort |
Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens |
author |
Perniconi, Sergio Eduardo |
author_facet |
Perniconi, Sergio Eduardo Simões, Manuel de Jesus Simões, Ricardo dos Santos Haidar, Mauro Abi Baracat, Edmund C Soares Jr, Jose Maria |
author_role |
author |
author2 |
Simões, Manuel de Jesus Simões, Ricardo dos Santos Haidar, Mauro Abi Baracat, Edmund C Soares Jr, Jose Maria |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Perniconi, Sergio Eduardo Simões, Manuel de Jesus Simões, Ricardo dos Santos Haidar, Mauro Abi Baracat, Edmund C Soares Jr, Jose Maria |
dc.subject.por.fl_str_mv |
Estrogens Superficial epithelium Ovary Animal Model |
topic |
Estrogens Superficial epithelium Ovary Animal Model |
description |
OBJECTIVE: To evaluate the effect of different concentrations of estrogen on the ovarian superficial epithelium in senile female rats. Design: Fifty female rats at 15 months of age and with irregular estrous cycles were selected and randomly divided into five experimental groups containing equal numbers of animals in each: GPROP, control group receiving vehicle only; GE0.05mg, group receiving conjugated equine estrogens (CEE) at a dose of 50 µg/kg; GE0.5mg, group receiving CEE at 500 µg/kg; GE1mg, group receiving CEE at 1 mg/kg; and GE2mg, receiving CEE at 2 mg/kg. The length of treatment was 21 days. After this period, the animals were anesthetized and the ovaries were fixed in 10% formaldehyde and processed for routine histology. Histomorphology was analyzed by light microscopy, and histomorphometrics were evaluated using the Imagelab program. RESULTS: In the GPROP and GE0.05mg groups, the superficial epithelium of the ovary had a simple cuboidal shape, and as the estrogen dose increased, the epithelium thickened, with pseudo-stratified or stratified epithelium appearing in the GE2mg group. The animals in the group given the highest estrogen dose (GE2mg) showed the thickest ovarian epithelium and the largest perimeter and surface area of the surface ovarian epithelium (P < 0.01). However, the difference in epithelium thickness between the GE0.5mg and GE1mg groups was only slight. CONCLUSION: Our data suggest that CEE at a dose of 2 mg/kg may induce marked proliferation of rat ovarian epithelium. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/17810 10.1590/S1807-59322008000300016 |
url |
https://www.revistas.usp.br/clinics/article/view/17810 |
identifier_str_mv |
10.1590/S1807-59322008000300016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/17810/19875 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 63 No. 3 (2008); 381-388 Clinics; v. 63 n. 3 (2008); 381-388 Clinics; Vol. 63 Núm. 3 (2008); 381-388 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222753908850688 |