Expression of tumor suppressors miR-195 and let-7a as potential biomarkers of invasive breast cancer
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/154702 |
Resumo: | OBJECTIVE: MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the posttranscriptional level. Some miRNAs, including let-7a and miR-195, have been described as tumor suppressors. However, the roles of these microRNAs in breast cancer progression remain controversial. The aim of this study is to evaluate miR-195 and let-7a expression as potential biomarkers of invasive breast cancer. METHODS: In the present study, 200 individuals were separated into three groups: (i) 72 women constituting the control group who were selected according to rigorous and well-established criteria; (ii) 56 patients with benign breast tumors; and (iii) 72 patients with malignant breast cancers of different clinical stages. The miR-195 and let-7a expression levels in serum were evaluated by real-time PCR. The results were assessed alone and in combination, and the analysis included an estimation of sensitivity and specificity in ROC curves. RESULTS: Compared with the benign and control groups, both microRNAs were downregulated in the malignant breast cancer patient group. Compared with the malignant group, the combination of both biomarkers in the control and benign groups showed good sensitivity and specificity in the serum with AUCs of 0.75 and 0.72, respectively. The biomarker combination for the control group versus the malignant group exhibited a better sensitivity and specificity than for the benign group versus the malignant group. CONCLUSION: These findings support the evidence that the analysis of miR-195 and let-7a can be used as a noninvasive biomarker for breast cancer detection. |
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oai:revistas.usp.br:article/154702 |
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Clinics |
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Expression of tumor suppressors miR-195 and let-7a as potential biomarkers of invasive breast cancerBreast CancerBiomarkersTumor SuppressorsEarly DetectionmicroRNAsOBJECTIVE: MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the posttranscriptional level. Some miRNAs, including let-7a and miR-195, have been described as tumor suppressors. However, the roles of these microRNAs in breast cancer progression remain controversial. The aim of this study is to evaluate miR-195 and let-7a expression as potential biomarkers of invasive breast cancer. METHODS: In the present study, 200 individuals were separated into three groups: (i) 72 women constituting the control group who were selected according to rigorous and well-established criteria; (ii) 56 patients with benign breast tumors; and (iii) 72 patients with malignant breast cancers of different clinical stages. The miR-195 and let-7a expression levels in serum were evaluated by real-time PCR. The results were assessed alone and in combination, and the analysis included an estimation of sensitivity and specificity in ROC curves. RESULTS: Compared with the benign and control groups, both microRNAs were downregulated in the malignant breast cancer patient group. Compared with the malignant group, the combination of both biomarkers in the control and benign groups showed good sensitivity and specificity in the serum with AUCs of 0.75 and 0.72, respectively. The biomarker combination for the control group versus the malignant group exhibited a better sensitivity and specificity than for the benign group versus the malignant group. CONCLUSION: These findings support the evidence that the analysis of miR-195 and let-7a can be used as a noninvasive biomarker for breast cancer detection.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2019-02-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/15470210.6061/clinics/2018/e184Clinics; Vol. 73 (2018); e184Clinics; v. 73 (2018); e184Clinics; Vol. 73 (2018); e1841980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/154702/150757Copyright (c) 2019 Clinicsinfo:eu-repo/semantics/openAccessMarques, Marcia M.Evangelista, Adriane F.Macedo, TacianeVieira, Rene Aloisio da CostaScapulatempo-Neto, CristovamReis, Rui M.Carvalho, André L.Silva, Ismael Dale Cotrim Guerreiro da2019-05-14T11:48:50Zoai:revistas.usp.br:article/154702Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2019-05-14T11:48:50Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Expression of tumor suppressors miR-195 and let-7a as potential biomarkers of invasive breast cancer |
title |
Expression of tumor suppressors miR-195 and let-7a as potential biomarkers of invasive breast cancer |
spellingShingle |
Expression of tumor suppressors miR-195 and let-7a as potential biomarkers of invasive breast cancer Marques, Marcia M. Breast Cancer Biomarkers Tumor Suppressors Early Detection microRNAs |
title_short |
Expression of tumor suppressors miR-195 and let-7a as potential biomarkers of invasive breast cancer |
title_full |
Expression of tumor suppressors miR-195 and let-7a as potential biomarkers of invasive breast cancer |
title_fullStr |
Expression of tumor suppressors miR-195 and let-7a as potential biomarkers of invasive breast cancer |
title_full_unstemmed |
Expression of tumor suppressors miR-195 and let-7a as potential biomarkers of invasive breast cancer |
title_sort |
Expression of tumor suppressors miR-195 and let-7a as potential biomarkers of invasive breast cancer |
author |
Marques, Marcia M. |
author_facet |
Marques, Marcia M. Evangelista, Adriane F. Macedo, Taciane Vieira, Rene Aloisio da Costa Scapulatempo-Neto, Cristovam Reis, Rui M. Carvalho, André L. Silva, Ismael Dale Cotrim Guerreiro da |
author_role |
author |
author2 |
Evangelista, Adriane F. Macedo, Taciane Vieira, Rene Aloisio da Costa Scapulatempo-Neto, Cristovam Reis, Rui M. Carvalho, André L. Silva, Ismael Dale Cotrim Guerreiro da |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Marques, Marcia M. Evangelista, Adriane F. Macedo, Taciane Vieira, Rene Aloisio da Costa Scapulatempo-Neto, Cristovam Reis, Rui M. Carvalho, André L. Silva, Ismael Dale Cotrim Guerreiro da |
dc.subject.por.fl_str_mv |
Breast Cancer Biomarkers Tumor Suppressors Early Detection microRNAs |
topic |
Breast Cancer Biomarkers Tumor Suppressors Early Detection microRNAs |
description |
OBJECTIVE: MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the posttranscriptional level. Some miRNAs, including let-7a and miR-195, have been described as tumor suppressors. However, the roles of these microRNAs in breast cancer progression remain controversial. The aim of this study is to evaluate miR-195 and let-7a expression as potential biomarkers of invasive breast cancer. METHODS: In the present study, 200 individuals were separated into three groups: (i) 72 women constituting the control group who were selected according to rigorous and well-established criteria; (ii) 56 patients with benign breast tumors; and (iii) 72 patients with malignant breast cancers of different clinical stages. The miR-195 and let-7a expression levels in serum were evaluated by real-time PCR. The results were assessed alone and in combination, and the analysis included an estimation of sensitivity and specificity in ROC curves. RESULTS: Compared with the benign and control groups, both microRNAs were downregulated in the malignant breast cancer patient group. Compared with the malignant group, the combination of both biomarkers in the control and benign groups showed good sensitivity and specificity in the serum with AUCs of 0.75 and 0.72, respectively. The biomarker combination for the control group versus the malignant group exhibited a better sensitivity and specificity than for the benign group versus the malignant group. CONCLUSION: These findings support the evidence that the analysis of miR-195 and let-7a can be used as a noninvasive biomarker for breast cancer detection. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-02-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/154702 10.6061/clinics/2018/e184 |
url |
https://www.revistas.usp.br/clinics/article/view/154702 |
identifier_str_mv |
10.6061/clinics/2018/e184 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/154702/150757 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 73 (2018); e184 Clinics; v. 73 (2018); e184 Clinics; Vol. 73 (2018); e184 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222763724570624 |