Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/18340 |
Resumo: | OBJECTIVE: Nitric oxide produced by endothelial nitric oxide synthase (eNOS) possesses multiple anti-atherosclerotic properties. Hence, enhanced expression of eNOS and increased Nitric oxide levels may protect against the development of atherosclerosis. Piper sarmentosum is a tropical plant with antioxidant and anti-inflammatory activities. This study aimed to investigate the effects of Piper sarmentosum on the eNOS and Nitric oxide pathway in cultured human umbilical vein endothelial cells (HUVECs). METHODS: HUVECs were divided into four groups: control, treatment with 180 μM hydrogen peroxide (H2O2), treatment with 150 μg/mL aqueous extract of Piper sarmentosum, and concomitant treatment with aqueous extract of PS and H2O2 for 24 hours. Subsequently, HUVECs were harvested and eNOS mRNA expression was determined using qPCR. The eNOS protein level was measured using ELISA, and the eNOS activity and Nitric oxide level were determined by the Griess reaction. RESULTS: Human umbilical vein endothelial cells treated with aqueous extract of Piper sarmentosum showed a marked induction of Nitric oxide. Treatment with PS also resulted in increased eNOS mRNA expression, eNOS protein level and eNOS activity in HUVECs. CONCLUSION: Aqueous extract of Piper sarmentosum may improve endothelial function by promoting NO production in HUVECs. |
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Clinics |
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Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells Piper sarmentosumNitric oxideEndothelial nitric oxide synthaseOxidative stressHuman umbilical vein endothelial cells OBJECTIVE: Nitric oxide produced by endothelial nitric oxide synthase (eNOS) possesses multiple anti-atherosclerotic properties. Hence, enhanced expression of eNOS and increased Nitric oxide levels may protect against the development of atherosclerosis. Piper sarmentosum is a tropical plant with antioxidant and anti-inflammatory activities. This study aimed to investigate the effects of Piper sarmentosum on the eNOS and Nitric oxide pathway in cultured human umbilical vein endothelial cells (HUVECs). METHODS: HUVECs were divided into four groups: control, treatment with 180 μM hydrogen peroxide (H2O2), treatment with 150 μg/mL aqueous extract of Piper sarmentosum, and concomitant treatment with aqueous extract of PS and H2O2 for 24 hours. Subsequently, HUVECs were harvested and eNOS mRNA expression was determined using qPCR. The eNOS protein level was measured using ELISA, and the eNOS activity and Nitric oxide level were determined by the Griess reaction. RESULTS: Human umbilical vein endothelial cells treated with aqueous extract of Piper sarmentosum showed a marked induction of Nitric oxide. Treatment with PS also resulted in increased eNOS mRNA expression, eNOS protein level and eNOS activity in HUVECs. CONCLUSION: Aqueous extract of Piper sarmentosum may improve endothelial function by promoting NO production in HUVECs. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1834010.1590/S1807-59322010000700010Clinics; Vol. 65 No. 7 (2010); 709-714 Clinics; v. 65 n. 7 (2010); 709-714 Clinics; Vol. 65 Núm. 7 (2010); 709-714 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/18340/20403Ugusman, AzizahZakaria, ZaitonHui, Chua KienNordin, Nor Anita Megat Mohdinfo:eu-repo/semantics/openAccess2012-05-23T11:14:47Zoai:revistas.usp.br:article/18340Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-23T11:14:47Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells |
title |
Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells |
spellingShingle |
Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells Ugusman, Azizah Piper sarmentosum Nitric oxide Endothelial nitric oxide synthase Oxidative stress Human umbilical vein endothelial cells |
title_short |
Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells |
title_full |
Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells |
title_fullStr |
Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells |
title_full_unstemmed |
Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells |
title_sort |
Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells |
author |
Ugusman, Azizah |
author_facet |
Ugusman, Azizah Zakaria, Zaiton Hui, Chua Kien Nordin, Nor Anita Megat Mohd |
author_role |
author |
author2 |
Zakaria, Zaiton Hui, Chua Kien Nordin, Nor Anita Megat Mohd |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Ugusman, Azizah Zakaria, Zaiton Hui, Chua Kien Nordin, Nor Anita Megat Mohd |
dc.subject.por.fl_str_mv |
Piper sarmentosum Nitric oxide Endothelial nitric oxide synthase Oxidative stress Human umbilical vein endothelial cells |
topic |
Piper sarmentosum Nitric oxide Endothelial nitric oxide synthase Oxidative stress Human umbilical vein endothelial cells |
description |
OBJECTIVE: Nitric oxide produced by endothelial nitric oxide synthase (eNOS) possesses multiple anti-atherosclerotic properties. Hence, enhanced expression of eNOS and increased Nitric oxide levels may protect against the development of atherosclerosis. Piper sarmentosum is a tropical plant with antioxidant and anti-inflammatory activities. This study aimed to investigate the effects of Piper sarmentosum on the eNOS and Nitric oxide pathway in cultured human umbilical vein endothelial cells (HUVECs). METHODS: HUVECs were divided into four groups: control, treatment with 180 μM hydrogen peroxide (H2O2), treatment with 150 μg/mL aqueous extract of Piper sarmentosum, and concomitant treatment with aqueous extract of PS and H2O2 for 24 hours. Subsequently, HUVECs were harvested and eNOS mRNA expression was determined using qPCR. The eNOS protein level was measured using ELISA, and the eNOS activity and Nitric oxide level were determined by the Griess reaction. RESULTS: Human umbilical vein endothelial cells treated with aqueous extract of Piper sarmentosum showed a marked induction of Nitric oxide. Treatment with PS also resulted in increased eNOS mRNA expression, eNOS protein level and eNOS activity in HUVECs. CONCLUSION: Aqueous extract of Piper sarmentosum may improve endothelial function by promoting NO production in HUVECs. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/18340 10.1590/S1807-59322010000700010 |
url |
https://www.revistas.usp.br/clinics/article/view/18340 |
identifier_str_mv |
10.1590/S1807-59322010000700010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/18340/20403 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 65 No. 7 (2010); 709-714 Clinics; v. 65 n. 7 (2010); 709-714 Clinics; Vol. 65 Núm. 7 (2010); 709-714 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222754831597568 |