Expression of dNK cells and their cytokines in twin pregnancies with preeclampsia
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/164111 |
Resumo: | OBJECTIVES: To assess the expression of decidual natural killer (dNK) cells and their cytokines in twin pregnancies with preeclampsia. METHODS: This was a prospective case-control study. The inclusion criteria were diamniotic (monochorionic or dichorionic) twin pregnancies in the third trimester with negative serological results for infectious diseases; absence of major fetal abnormalities or twin-twin transfusion syndrome; and no history of administration of corticosteroids in this pregnancy. The control group (CG) included uncomplicated twin pregnancies, and the preeclampsia group (PEG) included twin gestations with clinical and laboratory confirmation of the disease according to well-established criteria. Samples of the decidua were obtained and analyzed by immunohistochemistry for the expression of dNK cells and interleukins (ILs) 10, 12 and 15. In addition, maternal serum samples were collected to determine the levels of these interleukins. RESULTS: Thirty twin pregnancies were selected: 20 in the control group (CG) and 10 in the preeclampsia group (PEG). The PEG showed strong placental immunostaining for IL-15 (p=0.001) and high maternal serum levels of IL-10 (22.7 vs. 11.9 pg/mL, p=0.024) and IL-15 (15.9 vs. 7.4 pg/mL, p=0.024). CONCLUSION: A higher maternal serum concentration of both pro- and anti-inflammatory factors was observed in the twin pregnancies in the PEG. However, no difference in placental expression of IL-10 was found between the groups. These findings may suggest that maternal attempts to balance these interleukins were not sufficient to cause a placental response, and this failure may contribute to the development of preeclampsia. |
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Clinics |
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Expression of dNK cells and their cytokines in twin pregnancies with preeclampsiaTwin PregnancyPreeclampsiaDecidual Natural Killer CellsInterleukinsOBJECTIVES: To assess the expression of decidual natural killer (dNK) cells and their cytokines in twin pregnancies with preeclampsia. METHODS: This was a prospective case-control study. The inclusion criteria were diamniotic (monochorionic or dichorionic) twin pregnancies in the third trimester with negative serological results for infectious diseases; absence of major fetal abnormalities or twin-twin transfusion syndrome; and no history of administration of corticosteroids in this pregnancy. The control group (CG) included uncomplicated twin pregnancies, and the preeclampsia group (PEG) included twin gestations with clinical and laboratory confirmation of the disease according to well-established criteria. Samples of the decidua were obtained and analyzed by immunohistochemistry for the expression of dNK cells and interleukins (ILs) 10, 12 and 15. In addition, maternal serum samples were collected to determine the levels of these interleukins. RESULTS: Thirty twin pregnancies were selected: 20 in the control group (CG) and 10 in the preeclampsia group (PEG). The PEG showed strong placental immunostaining for IL-15 (p=0.001) and high maternal serum levels of IL-10 (22.7 vs. 11.9 pg/mL, p=0.024) and IL-15 (15.9 vs. 7.4 pg/mL, p=0.024). CONCLUSION: A higher maternal serum concentration of both pro- and anti-inflammatory factors was observed in the twin pregnancies in the PEG. However, no difference in placental expression of IL-10 was found between the groups. These findings may suggest that maternal attempts to balance these interleukins were not sufficient to cause a placental response, and this failure may contribute to the development of preeclampsia.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2019-11-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/clinics/article/view/16411110.6061/clinics/2019/e1200Clinics; Vol. 74 (2019); e1200Clinics; v. 74 (2019); e1200Clinics; Vol. 74 (2019); e12001980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/164111/157547https://www.revistas.usp.br/clinics/article/view/164111/157548Copyright (c) 2019 Clinicsinfo:eu-repo/semantics/openAccessAgra, Isabela K.R.Liao, Adolfo W.Hoshida, Mara S.Schultz, ReginaToscano, Marcello P.Francisco, Rossana P.V.Zugaib, MarceloBrizot, Maria L.2019-11-14T18:19:34Zoai:revistas.usp.br:article/164111Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2019-11-14T18:19:34Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Expression of dNK cells and their cytokines in twin pregnancies with preeclampsia |
title |
Expression of dNK cells and their cytokines in twin pregnancies with preeclampsia |
spellingShingle |
Expression of dNK cells and their cytokines in twin pregnancies with preeclampsia Agra, Isabela K.R. Twin Pregnancy Preeclampsia Decidual Natural Killer Cells Interleukins |
title_short |
Expression of dNK cells and their cytokines in twin pregnancies with preeclampsia |
title_full |
Expression of dNK cells and their cytokines in twin pregnancies with preeclampsia |
title_fullStr |
Expression of dNK cells and their cytokines in twin pregnancies with preeclampsia |
title_full_unstemmed |
Expression of dNK cells and their cytokines in twin pregnancies with preeclampsia |
title_sort |
Expression of dNK cells and their cytokines in twin pregnancies with preeclampsia |
author |
Agra, Isabela K.R. |
author_facet |
Agra, Isabela K.R. Liao, Adolfo W. Hoshida, Mara S. Schultz, Regina Toscano, Marcello P. Francisco, Rossana P.V. Zugaib, Marcelo Brizot, Maria L. |
author_role |
author |
author2 |
Liao, Adolfo W. Hoshida, Mara S. Schultz, Regina Toscano, Marcello P. Francisco, Rossana P.V. Zugaib, Marcelo Brizot, Maria L. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Agra, Isabela K.R. Liao, Adolfo W. Hoshida, Mara S. Schultz, Regina Toscano, Marcello P. Francisco, Rossana P.V. Zugaib, Marcelo Brizot, Maria L. |
dc.subject.por.fl_str_mv |
Twin Pregnancy Preeclampsia Decidual Natural Killer Cells Interleukins |
topic |
Twin Pregnancy Preeclampsia Decidual Natural Killer Cells Interleukins |
description |
OBJECTIVES: To assess the expression of decidual natural killer (dNK) cells and their cytokines in twin pregnancies with preeclampsia. METHODS: This was a prospective case-control study. The inclusion criteria were diamniotic (monochorionic or dichorionic) twin pregnancies in the third trimester with negative serological results for infectious diseases; absence of major fetal abnormalities or twin-twin transfusion syndrome; and no history of administration of corticosteroids in this pregnancy. The control group (CG) included uncomplicated twin pregnancies, and the preeclampsia group (PEG) included twin gestations with clinical and laboratory confirmation of the disease according to well-established criteria. Samples of the decidua were obtained and analyzed by immunohistochemistry for the expression of dNK cells and interleukins (ILs) 10, 12 and 15. In addition, maternal serum samples were collected to determine the levels of these interleukins. RESULTS: Thirty twin pregnancies were selected: 20 in the control group (CG) and 10 in the preeclampsia group (PEG). The PEG showed strong placental immunostaining for IL-15 (p=0.001) and high maternal serum levels of IL-10 (22.7 vs. 11.9 pg/mL, p=0.024) and IL-15 (15.9 vs. 7.4 pg/mL, p=0.024). CONCLUSION: A higher maternal serum concentration of both pro- and anti-inflammatory factors was observed in the twin pregnancies in the PEG. However, no difference in placental expression of IL-10 was found between the groups. These findings may suggest that maternal attempts to balance these interleukins were not sufficient to cause a placental response, and this failure may contribute to the development of preeclampsia. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/164111 10.6061/clinics/2019/e1200 |
url |
https://www.revistas.usp.br/clinics/article/view/164111 |
identifier_str_mv |
10.6061/clinics/2019/e1200 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/164111/157547 https://www.revistas.usp.br/clinics/article/view/164111/157548 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/xml |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 74 (2019); e1200 Clinics; v. 74 (2019); e1200 Clinics; Vol. 74 (2019); e1200 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222764646268928 |