Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?

Detalhes bibliográficos
Autor(a) principal: Rossi, Flavia
Data de Publicação: 2017
Outros Autores: Girardello, Raquel, Morais, Carlos, Cury, Ana Paula, Martins, Layla Farage, da Silva, Aline Maria, Abdala, Edson, Setubal, João Carlos, da Silva Duarte, Alberto José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
DOI: 10.6061/clinics/2017(10)09
Texto Completo: https://www.revistas.usp.br/clinics/article/view/141021
Resumo: OBJECTIVE: We describe an IncX4 pHC891/16mcr plasmid carrying mcr-1 in a colistin-resistant and carbapenem-susceptible E. coli isolate (HC891/16), ST156, which caused a blood infection in a Brazilian patient with gallbladder adenocarcinoma. METHODS: Strain HC891/16 was subjected to whole genome sequencing using the MiSeq Platform (Illumina, Inc., USA). Assembly was performed using Mira and ABACAS. RESULTS: The isolates showed resistance only to ciprofloxacin, ampicillin and cefoxitin, and whole-genome sequencing revealed the presence of aac(6’)Ib-cr and blaTEM1. CONCLUSION: Our findings warn of the possible silent dissemination of colistin resistance by carbapenem-susceptible mcr-1 producers, as colistin susceptibility is commonly tested only among carbapenem-resistant isolates.
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spelling Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?Colistin ResistancePolymyxin ResistanceEscherichia ColiCarbapenem-SusceptibleST156OBJECTIVE: We describe an IncX4 pHC891/16mcr plasmid carrying mcr-1 in a colistin-resistant and carbapenem-susceptible E. coli isolate (HC891/16), ST156, which caused a blood infection in a Brazilian patient with gallbladder adenocarcinoma. METHODS: Strain HC891/16 was subjected to whole genome sequencing using the MiSeq Platform (Illumina, Inc., USA). Assembly was performed using Mira and ABACAS. RESULTS: The isolates showed resistance only to ciprofloxacin, ampicillin and cefoxitin, and whole-genome sequencing revealed the presence of aac(6’)Ib-cr and blaTEM1. CONCLUSION: Our findings warn of the possible silent dissemination of colistin resistance by carbapenem-susceptible mcr-1 producers, as colistin susceptibility is commonly tested only among carbapenem-resistant isolates.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2017-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/14102110.6061/clinics/2017(10)09Clinics; Vol. 72 No. 10 (2017); 642-644Clinics; v. 72 n. 10 (2017); 642-644Clinics; Vol. 72 Núm. 10 (2017); 642-6441980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/141021/136087Copyright (c) 2017 Clinicsinfo:eu-repo/semantics/openAccessRossi, FlaviaGirardello, RaquelMorais, CarlosCury, Ana PaulaMartins, Layla Farageda Silva, Aline MariaAbdala, EdsonSetubal, João Carlosda Silva Duarte, Alberto José2017-11-27T13:14:34Zoai:revistas.usp.br:article/141021Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2017-11-27T13:14:34Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
title Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
spellingShingle Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
Rossi, Flavia
Colistin Resistance
Polymyxin Resistance
Escherichia Coli
Carbapenem-Susceptible
ST156
Rossi, Flavia
Colistin Resistance
Polymyxin Resistance
Escherichia Coli
Carbapenem-Susceptible
ST156
title_short Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
title_full Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
title_fullStr Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
title_full_unstemmed Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
title_sort Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
author Rossi, Flavia
author_facet Rossi, Flavia
Rossi, Flavia
Girardello, Raquel
Morais, Carlos
Cury, Ana Paula
Martins, Layla Farage
da Silva, Aline Maria
Abdala, Edson
Setubal, João Carlos
da Silva Duarte, Alberto José
Girardello, Raquel
Morais, Carlos
Cury, Ana Paula
Martins, Layla Farage
da Silva, Aline Maria
Abdala, Edson
Setubal, João Carlos
da Silva Duarte, Alberto José
author_role author
author2 Girardello, Raquel
Morais, Carlos
Cury, Ana Paula
Martins, Layla Farage
da Silva, Aline Maria
Abdala, Edson
Setubal, João Carlos
da Silva Duarte, Alberto José
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rossi, Flavia
Girardello, Raquel
Morais, Carlos
Cury, Ana Paula
Martins, Layla Farage
da Silva, Aline Maria
Abdala, Edson
Setubal, João Carlos
da Silva Duarte, Alberto José
dc.subject.por.fl_str_mv Colistin Resistance
Polymyxin Resistance
Escherichia Coli
Carbapenem-Susceptible
ST156
topic Colistin Resistance
Polymyxin Resistance
Escherichia Coli
Carbapenem-Susceptible
ST156
description OBJECTIVE: We describe an IncX4 pHC891/16mcr plasmid carrying mcr-1 in a colistin-resistant and carbapenem-susceptible E. coli isolate (HC891/16), ST156, which caused a blood infection in a Brazilian patient with gallbladder adenocarcinoma. METHODS: Strain HC891/16 was subjected to whole genome sequencing using the MiSeq Platform (Illumina, Inc., USA). Assembly was performed using Mira and ABACAS. RESULTS: The isolates showed resistance only to ciprofloxacin, ampicillin and cefoxitin, and whole-genome sequencing revealed the presence of aac(6’)Ib-cr and blaTEM1. CONCLUSION: Our findings warn of the possible silent dissemination of colistin resistance by carbapenem-susceptible mcr-1 producers, as colistin susceptibility is commonly tested only among carbapenem-resistant isolates.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/141021
10.6061/clinics/2017(10)09
url https://www.revistas.usp.br/clinics/article/view/141021
identifier_str_mv 10.6061/clinics/2017(10)09
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/141021/136087
dc.rights.driver.fl_str_mv Copyright (c) 2017 Clinics
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Clinics
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 72 No. 10 (2017); 642-644
Clinics; v. 72 n. 10 (2017); 642-644
Clinics; Vol. 72 Núm. 10 (2017); 642-644
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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dc.identifier.doi.none.fl_str_mv 10.6061/clinics/2017(10)09

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