Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/19274 |
Resumo: | OBJECTIVE: To study the effects of mycophenolate sodium on mucociliary clearance. INTRODUCTION: Mycophenolate is one of the most commonly used immunosuppressive drugs in lung transplantation. Although its pharmacokinetic properties are well defined, its side effects on mucociliary clearance have not yet been studied. METHODS: Sixty rats were subjected to left bronchial section and anastomosis. The right bronchus was used as a control. After surgery, the rats were assigned to two groups based on whether they received saline solution (n = 30) or mycophenolate sodium (n = 30). After 7, 15, or 30 days of treatment, 10 animals from each group were sacrificed, and in vitro mucus transportability, in situ mucociliary transport velocity and ciliary beat frequency were measured. RESULTS: The analysis of mucus transportability revealed that neither mycophenolate nor bronchial section altered any transportability related property for up to 30 days of treatment after surgery (p>;0.05). With regard to ciliary beat frequency, the operated left bronchi from the mycophenolate group showed a significant decrease on post-surgical day 30 (p = 0.003). In addition, we found a significant reduction in the in situ mucociliary transport velocity in the mycophenolate-treated group (p = 0.0001). DISCUSSION: These data add important information regarding mucociliary clearance dysfunction following mycophenolate therapy and suggest that mycophenolate might contribute to the high incidence of respiratory tract infections in lung transplant patients. Further studies are needed to investigate the combined action of mycophenolate with other immunosuppressive drugs and to establish methods to protect and recover mucociliary clearance, an important airway defense mechanism. |
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Clinics |
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Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model Immunosuppressive AgentsLung TransplantationMucociliary ClearanceAnimals OBJECTIVE: To study the effects of mycophenolate sodium on mucociliary clearance. INTRODUCTION: Mycophenolate is one of the most commonly used immunosuppressive drugs in lung transplantation. Although its pharmacokinetic properties are well defined, its side effects on mucociliary clearance have not yet been studied. METHODS: Sixty rats were subjected to left bronchial section and anastomosis. The right bronchus was used as a control. After surgery, the rats were assigned to two groups based on whether they received saline solution (n = 30) or mycophenolate sodium (n = 30). After 7, 15, or 30 days of treatment, 10 animals from each group were sacrificed, and in vitro mucus transportability, in situ mucociliary transport velocity and ciliary beat frequency were measured. RESULTS: The analysis of mucus transportability revealed that neither mycophenolate nor bronchial section altered any transportability related property for up to 30 days of treatment after surgery (p>;0.05). With regard to ciliary beat frequency, the operated left bronchi from the mycophenolate group showed a significant decrease on post-surgical day 30 (p = 0.003). In addition, we found a significant reduction in the in situ mucociliary transport velocity in the mycophenolate-treated group (p = 0.0001). DISCUSSION: These data add important information regarding mucociliary clearance dysfunction following mycophenolate therapy and suggest that mycophenolate might contribute to the high incidence of respiratory tract infections in lung transplant patients. Further studies are needed to investigate the combined action of mycophenolate with other immunosuppressive drugs and to establish methods to protect and recover mucociliary clearance, an important airway defense mechanism. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1927410.1590/S1807-59322011000800024Clinics; Vol. 66 No. 8 (2011); 1451-1456 Clinics; v. 66 n. 8 (2011); 1451-1456 Clinics; Vol. 66 Núm. 8 (2011); 1451-1456 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/19274/21337Silva, Viviane Ferreira Paes ePazetti, RogerioSoto, Sonia de FatimaSiqueira, Mariana Moreira QuinhonesCorreia, Aristides TadeuJatene, Fabio BiscegliPego-Fernandes, Paulo Manuelinfo:eu-repo/semantics/openAccess2012-05-23T16:31:32Zoai:revistas.usp.br:article/19274Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-23T16:31:32Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model |
title |
Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model |
spellingShingle |
Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model Silva, Viviane Ferreira Paes e Immunosuppressive Agents Lung Transplantation Mucociliary Clearance Animals |
title_short |
Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model |
title_full |
Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model |
title_fullStr |
Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model |
title_full_unstemmed |
Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model |
title_sort |
Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model |
author |
Silva, Viviane Ferreira Paes e |
author_facet |
Silva, Viviane Ferreira Paes e Pazetti, Rogerio Soto, Sonia de Fatima Siqueira, Mariana Moreira Quinhones Correia, Aristides Tadeu Jatene, Fabio Biscegli Pego-Fernandes, Paulo Manuel |
author_role |
author |
author2 |
Pazetti, Rogerio Soto, Sonia de Fatima Siqueira, Mariana Moreira Quinhones Correia, Aristides Tadeu Jatene, Fabio Biscegli Pego-Fernandes, Paulo Manuel |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Viviane Ferreira Paes e Pazetti, Rogerio Soto, Sonia de Fatima Siqueira, Mariana Moreira Quinhones Correia, Aristides Tadeu Jatene, Fabio Biscegli Pego-Fernandes, Paulo Manuel |
dc.subject.por.fl_str_mv |
Immunosuppressive Agents Lung Transplantation Mucociliary Clearance Animals |
topic |
Immunosuppressive Agents Lung Transplantation Mucociliary Clearance Animals |
description |
OBJECTIVE: To study the effects of mycophenolate sodium on mucociliary clearance. INTRODUCTION: Mycophenolate is one of the most commonly used immunosuppressive drugs in lung transplantation. Although its pharmacokinetic properties are well defined, its side effects on mucociliary clearance have not yet been studied. METHODS: Sixty rats were subjected to left bronchial section and anastomosis. The right bronchus was used as a control. After surgery, the rats were assigned to two groups based on whether they received saline solution (n = 30) or mycophenolate sodium (n = 30). After 7, 15, or 30 days of treatment, 10 animals from each group were sacrificed, and in vitro mucus transportability, in situ mucociliary transport velocity and ciliary beat frequency were measured. RESULTS: The analysis of mucus transportability revealed that neither mycophenolate nor bronchial section altered any transportability related property for up to 30 days of treatment after surgery (p>;0.05). With regard to ciliary beat frequency, the operated left bronchi from the mycophenolate group showed a significant decrease on post-surgical day 30 (p = 0.003). In addition, we found a significant reduction in the in situ mucociliary transport velocity in the mycophenolate-treated group (p = 0.0001). DISCUSSION: These data add important information regarding mucociliary clearance dysfunction following mycophenolate therapy and suggest that mycophenolate might contribute to the high incidence of respiratory tract infections in lung transplant patients. Further studies are needed to investigate the combined action of mycophenolate with other immunosuppressive drugs and to establish methods to protect and recover mucociliary clearance, an important airway defense mechanism. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/19274 10.1590/S1807-59322011000800024 |
url |
https://www.revistas.usp.br/clinics/article/view/19274 |
identifier_str_mv |
10.1590/S1807-59322011000800024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/19274/21337 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 66 No. 8 (2011); 1451-1456 Clinics; v. 66 n. 8 (2011); 1451-1456 Clinics; Vol. 66 Núm. 8 (2011); 1451-1456 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222756345741312 |