Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model

Detalhes bibliográficos
Autor(a) principal: Silva, Viviane Ferreira Paes e
Data de Publicação: 2011
Outros Autores: Pazetti, Rogerio, Soto, Sonia de Fatima, Siqueira, Mariana Moreira Quinhones, Correia, Aristides Tadeu, Jatene, Fabio Biscegli, Pego-Fernandes, Paulo Manuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/19274
Resumo: OBJECTIVE: To study the effects of mycophenolate sodium on mucociliary clearance. INTRODUCTION: Mycophenolate is one of the most commonly used immunosuppressive drugs in lung transplantation. Although its pharmacokinetic properties are well defined, its side effects on mucociliary clearance have not yet been studied. METHODS: Sixty rats were subjected to left bronchial section and anastomosis. The right bronchus was used as a control. After surgery, the rats were assigned to two groups based on whether they received saline solution (n = 30) or mycophenolate sodium (n = 30). After 7, 15, or 30 days of treatment, 10 animals from each group were sacrificed, and in vitro mucus transportability, in situ mucociliary transport velocity and ciliary beat frequency were measured. RESULTS: The analysis of mucus transportability revealed that neither mycophenolate nor bronchial section altered any transportability related property for up to 30 days of treatment after surgery (p>;0.05). With regard to ciliary beat frequency, the operated left bronchi from the mycophenolate group showed a significant decrease on post-surgical day 30 (p = 0.003). In addition, we found a significant reduction in the in situ mucociliary transport velocity in the mycophenolate-treated group (p = 0.0001). DISCUSSION: These data add important information regarding mucociliary clearance dysfunction following mycophenolate therapy and suggest that mycophenolate might contribute to the high incidence of respiratory tract infections in lung transplant patients. Further studies are needed to investigate the combined action of mycophenolate with other immunosuppressive drugs and to establish methods to protect and recover mucociliary clearance, an important airway defense mechanism.
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spelling Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model Immunosuppressive AgentsLung TransplantationMucociliary ClearanceAnimals OBJECTIVE: To study the effects of mycophenolate sodium on mucociliary clearance. INTRODUCTION: Mycophenolate is one of the most commonly used immunosuppressive drugs in lung transplantation. Although its pharmacokinetic properties are well defined, its side effects on mucociliary clearance have not yet been studied. METHODS: Sixty rats were subjected to left bronchial section and anastomosis. The right bronchus was used as a control. After surgery, the rats were assigned to two groups based on whether they received saline solution (n = 30) or mycophenolate sodium (n = 30). After 7, 15, or 30 days of treatment, 10 animals from each group were sacrificed, and in vitro mucus transportability, in situ mucociliary transport velocity and ciliary beat frequency were measured. RESULTS: The analysis of mucus transportability revealed that neither mycophenolate nor bronchial section altered any transportability related property for up to 30 days of treatment after surgery (p>;0.05). With regard to ciliary beat frequency, the operated left bronchi from the mycophenolate group showed a significant decrease on post-surgical day 30 (p = 0.003). In addition, we found a significant reduction in the in situ mucociliary transport velocity in the mycophenolate-treated group (p = 0.0001). DISCUSSION: These data add important information regarding mucociliary clearance dysfunction following mycophenolate therapy and suggest that mycophenolate might contribute to the high incidence of respiratory tract infections in lung transplant patients. Further studies are needed to investigate the combined action of mycophenolate with other immunosuppressive drugs and to establish methods to protect and recover mucociliary clearance, an important airway defense mechanism. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1927410.1590/S1807-59322011000800024Clinics; Vol. 66 No. 8 (2011); 1451-1456 Clinics; v. 66 n. 8 (2011); 1451-1456 Clinics; Vol. 66 Núm. 8 (2011); 1451-1456 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/19274/21337Silva, Viviane Ferreira Paes ePazetti, RogerioSoto, Sonia de FatimaSiqueira, Mariana Moreira QuinhonesCorreia, Aristides TadeuJatene, Fabio BiscegliPego-Fernandes, Paulo Manuelinfo:eu-repo/semantics/openAccess2012-05-23T16:31:32Zoai:revistas.usp.br:article/19274Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-23T16:31:32Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model
title Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model
spellingShingle Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model
Silva, Viviane Ferreira Paes e
Immunosuppressive Agents
Lung Transplantation
Mucociliary Clearance
Animals
title_short Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model
title_full Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model
title_fullStr Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model
title_full_unstemmed Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model
title_sort Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model
author Silva, Viviane Ferreira Paes e
author_facet Silva, Viviane Ferreira Paes e
Pazetti, Rogerio
Soto, Sonia de Fatima
Siqueira, Mariana Moreira Quinhones
Correia, Aristides Tadeu
Jatene, Fabio Biscegli
Pego-Fernandes, Paulo Manuel
author_role author
author2 Pazetti, Rogerio
Soto, Sonia de Fatima
Siqueira, Mariana Moreira Quinhones
Correia, Aristides Tadeu
Jatene, Fabio Biscegli
Pego-Fernandes, Paulo Manuel
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Viviane Ferreira Paes e
Pazetti, Rogerio
Soto, Sonia de Fatima
Siqueira, Mariana Moreira Quinhones
Correia, Aristides Tadeu
Jatene, Fabio Biscegli
Pego-Fernandes, Paulo Manuel
dc.subject.por.fl_str_mv Immunosuppressive Agents
Lung Transplantation
Mucociliary Clearance
Animals
topic Immunosuppressive Agents
Lung Transplantation
Mucociliary Clearance
Animals
description OBJECTIVE: To study the effects of mycophenolate sodium on mucociliary clearance. INTRODUCTION: Mycophenolate is one of the most commonly used immunosuppressive drugs in lung transplantation. Although its pharmacokinetic properties are well defined, its side effects on mucociliary clearance have not yet been studied. METHODS: Sixty rats were subjected to left bronchial section and anastomosis. The right bronchus was used as a control. After surgery, the rats were assigned to two groups based on whether they received saline solution (n = 30) or mycophenolate sodium (n = 30). After 7, 15, or 30 days of treatment, 10 animals from each group were sacrificed, and in vitro mucus transportability, in situ mucociliary transport velocity and ciliary beat frequency were measured. RESULTS: The analysis of mucus transportability revealed that neither mycophenolate nor bronchial section altered any transportability related property for up to 30 days of treatment after surgery (p>;0.05). With regard to ciliary beat frequency, the operated left bronchi from the mycophenolate group showed a significant decrease on post-surgical day 30 (p = 0.003). In addition, we found a significant reduction in the in situ mucociliary transport velocity in the mycophenolate-treated group (p = 0.0001). DISCUSSION: These data add important information regarding mucociliary clearance dysfunction following mycophenolate therapy and suggest that mycophenolate might contribute to the high incidence of respiratory tract infections in lung transplant patients. Further studies are needed to investigate the combined action of mycophenolate with other immunosuppressive drugs and to establish methods to protect and recover mucociliary clearance, an important airway defense mechanism.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/19274
10.1590/S1807-59322011000800024
url https://www.revistas.usp.br/clinics/article/view/19274
identifier_str_mv 10.1590/S1807-59322011000800024
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/19274/21337
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 66 No. 8 (2011); 1451-1456
Clinics; v. 66 n. 8 (2011); 1451-1456
Clinics; Vol. 66 Núm. 8 (2011); 1451-1456
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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