Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/157954 |
Resumo: | OBJECTIVE: To report our experience using conventional culture methods (CM) and pediatric blood culture bottles (PBCBs) for vitreous sample culture of acute postoperative endophthalmitis. METHODS: A retrospective study was conducted at the Department of Ophthalmology, Hospital das Clinicas, HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, BR, from January 2010 to December 2015, and it included 54 patients with clinically suspected acute postoperative endophthalmitis. Vitreous samples were obtained by vitreous tap or vitrectomy. Samples from January 2010 to December 2011 were cultivated in CM, whereas samples from January 2012 to December 2015 were inoculated in PBCBs. The measured outcome was the yield of positive cultures. RESULTS: Twenty cases were included in the CM group, and 34 cases were included in the PBCB group. The yield of positive cultures in PBCBs (64.7%) was significantly higher than that in conventional CM (35%, p=0.034). Staphylococcus epidermidis and Streptococcus viridans were the two most commonly found agents. CONCLUSION: PBCBs can be used successfully in clinically suspected endophthalmitis. The method showed a higher yield of positive cultures than the conventional method. This technique appears to have several advantages over the traditional method: it saves time, as only one medium needs to be inoculated; transportation to a laboratory is easier than in the traditional method, and there is no need to maintain a supply of fresh agar media. The use of PBCBs may be recommended as the primary method for microbiological diagnosis and is especially suitable for office settings and remote clinics. |
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Clinics |
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Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitisEndophthalmitisConventional CultureBlood Culture BottlePostoperative ComplicationsOBJECTIVE: To report our experience using conventional culture methods (CM) and pediatric blood culture bottles (PBCBs) for vitreous sample culture of acute postoperative endophthalmitis. METHODS: A retrospective study was conducted at the Department of Ophthalmology, Hospital das Clinicas, HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, BR, from January 2010 to December 2015, and it included 54 patients with clinically suspected acute postoperative endophthalmitis. Vitreous samples were obtained by vitreous tap or vitrectomy. Samples from January 2010 to December 2011 were cultivated in CM, whereas samples from January 2012 to December 2015 were inoculated in PBCBs. The measured outcome was the yield of positive cultures. RESULTS: Twenty cases were included in the CM group, and 34 cases were included in the PBCB group. The yield of positive cultures in PBCBs (64.7%) was significantly higher than that in conventional CM (35%, p=0.034). Staphylococcus epidermidis and Streptococcus viridans were the two most commonly found agents. CONCLUSION: PBCBs can be used successfully in clinically suspected endophthalmitis. The method showed a higher yield of positive cultures than the conventional method. This technique appears to have several advantages over the traditional method: it saves time, as only one medium needs to be inoculated; transportation to a laboratory is easier than in the traditional method, and there is no need to maintain a supply of fresh agar media. The use of PBCBs may be recommended as the primary method for microbiological diagnosis and is especially suitable for office settings and remote clinics.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2019-05-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/clinics/article/view/15795410.6061/clinics/2019/e837Clinics; Vol. 74 (2019); e837Clinics; v. 74 (2019); e837Clinics; Vol. 74 (2019); e8371980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/157954/153235https://www.revistas.usp.br/clinics/article/view/157954/153572Copyright (c) 2019 Clinicsinfo:eu-repo/semantics/openAccessTanaka, TatianaFerreira, Bruno Fortaleza de AquinoOliveira, Luiza Manhezi Shin deKato, Juliana MikaDi Gioia, Thais Sabato RomanoRossi, FlaviaNakashima, YoshitakaPimentel, Sergio Luis GianottiYamamoto, Joyce HisaeAlmeida Junior, João Nobrega de2019-05-24T13:16:00Zoai:revistas.usp.br:article/157954Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2019-05-24T13:16Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis |
title |
Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis |
spellingShingle |
Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis Tanaka, Tatiana Endophthalmitis Conventional Culture Blood Culture Bottle Postoperative Complications |
title_short |
Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis |
title_full |
Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis |
title_fullStr |
Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis |
title_full_unstemmed |
Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis |
title_sort |
Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis |
author |
Tanaka, Tatiana |
author_facet |
Tanaka, Tatiana Ferreira, Bruno Fortaleza de Aquino Oliveira, Luiza Manhezi Shin de Kato, Juliana Mika Di Gioia, Thais Sabato Romano Rossi, Flavia Nakashima, Yoshitaka Pimentel, Sergio Luis Gianotti Yamamoto, Joyce Hisae Almeida Junior, João Nobrega de |
author_role |
author |
author2 |
Ferreira, Bruno Fortaleza de Aquino Oliveira, Luiza Manhezi Shin de Kato, Juliana Mika Di Gioia, Thais Sabato Romano Rossi, Flavia Nakashima, Yoshitaka Pimentel, Sergio Luis Gianotti Yamamoto, Joyce Hisae Almeida Junior, João Nobrega de |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Tanaka, Tatiana Ferreira, Bruno Fortaleza de Aquino Oliveira, Luiza Manhezi Shin de Kato, Juliana Mika Di Gioia, Thais Sabato Romano Rossi, Flavia Nakashima, Yoshitaka Pimentel, Sergio Luis Gianotti Yamamoto, Joyce Hisae Almeida Junior, João Nobrega de |
dc.subject.por.fl_str_mv |
Endophthalmitis Conventional Culture Blood Culture Bottle Postoperative Complications |
topic |
Endophthalmitis Conventional Culture Blood Culture Bottle Postoperative Complications |
description |
OBJECTIVE: To report our experience using conventional culture methods (CM) and pediatric blood culture bottles (PBCBs) for vitreous sample culture of acute postoperative endophthalmitis. METHODS: A retrospective study was conducted at the Department of Ophthalmology, Hospital das Clinicas, HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, BR, from January 2010 to December 2015, and it included 54 patients with clinically suspected acute postoperative endophthalmitis. Vitreous samples were obtained by vitreous tap or vitrectomy. Samples from January 2010 to December 2011 were cultivated in CM, whereas samples from January 2012 to December 2015 were inoculated in PBCBs. The measured outcome was the yield of positive cultures. RESULTS: Twenty cases were included in the CM group, and 34 cases were included in the PBCB group. The yield of positive cultures in PBCBs (64.7%) was significantly higher than that in conventional CM (35%, p=0.034). Staphylococcus epidermidis and Streptococcus viridans were the two most commonly found agents. CONCLUSION: PBCBs can be used successfully in clinically suspected endophthalmitis. The method showed a higher yield of positive cultures than the conventional method. This technique appears to have several advantages over the traditional method: it saves time, as only one medium needs to be inoculated; transportation to a laboratory is easier than in the traditional method, and there is no need to maintain a supply of fresh agar media. The use of PBCBs may be recommended as the primary method for microbiological diagnosis and is especially suitable for office settings and remote clinics. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/157954 10.6061/clinics/2019/e837 |
url |
https://www.revistas.usp.br/clinics/article/view/157954 |
identifier_str_mv |
10.6061/clinics/2019/e837 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/157954/153235 https://www.revistas.usp.br/clinics/article/view/157954/153572 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/xml |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 74 (2019); e837 Clinics; v. 74 (2019); e837 Clinics; Vol. 74 (2019); e837 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222764128272384 |