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Evaluation of the effects of erythropoietin and interleukin-6 in rats submitted to acute spinal cord injury

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Autor(a) principal: Barros, Alderico Girão Campos de
Data de Publicação: 2019
Outros Autores: Cristante, Alexandre Fogaça, Santos, Gustavo Bispo dos, Natalino, Renato José Mendonça, Ferreira, Ricardo José Rodriguez, Barros-Filho, Tarcísio Eloy Pessoa de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/161429
Resumo: OBJECTIVE: To evaluate the effects of interleukin-6 (IL-6) and erythropoietin (EPO) in experimental acute spinal cord injury (SCI) in rats. METHODS: Using standardized equipment, namely, a New York University (NYU) Impactor, a SCI was produced in 50 Wistar rats using a 10-g weight drop from a 12.5-mm height. The rats were divided into the following 5 groups of 10 animals each: ‘‘Group EPO’’, treated with erythropoietin only; ‘‘Group EPO + IL-6’’, treated with both substances; ‘‘Group IL-6’’, receiving IL-6 administration only; ‘‘Group Placebo’’, receiving a placebo solution; and ‘‘Group Sham’’, submitted to an incomplete procedure (only laminectomy, without SCI). All drugs and the placebo solution were administered intraperitoneally for three weeks. The animals were followed up for 42 days. Functional motor recovery was monitored by the Basso, Beattie, and Bresnahan (BBB) scale on days 2, 7, 14, 21, 28, 35 and 42. Motor-evoked potential tests were performed on the 42nd day. Histological analysis was performed after euthanasia. RESULTS: The group receiving EPO exhibited superior functional motor results on the BBB scale. IL-6 administration alone was not superior to the placebo treatment, and the IL-6 combination with EPO yielded worse results than did EPO alone. CONCLUSIONS: Using EPO after acute SCI in rats yielded benefits in functional recovery. The combination of EPO and IL-6 showed benefits, but with inferior results compared to those of isolated EPO; moreover, isolated use of IL-6 resulted in no benefit.
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spelling Evaluation of the effects of erythropoietin and interleukin-6 in rats submitted to acute spinal cord injuryInterleukin-6ErythropoietinSpinal Cord InjuriesCentral Nervous System/InjuriesRatsOBJECTIVE: To evaluate the effects of interleukin-6 (IL-6) and erythropoietin (EPO) in experimental acute spinal cord injury (SCI) in rats. METHODS: Using standardized equipment, namely, a New York University (NYU) Impactor, a SCI was produced in 50 Wistar rats using a 10-g weight drop from a 12.5-mm height. The rats were divided into the following 5 groups of 10 animals each: ‘‘Group EPO’’, treated with erythropoietin only; ‘‘Group EPO + IL-6’’, treated with both substances; ‘‘Group IL-6’’, receiving IL-6 administration only; ‘‘Group Placebo’’, receiving a placebo solution; and ‘‘Group Sham’’, submitted to an incomplete procedure (only laminectomy, without SCI). All drugs and the placebo solution were administered intraperitoneally for three weeks. The animals were followed up for 42 days. Functional motor recovery was monitored by the Basso, Beattie, and Bresnahan (BBB) scale on days 2, 7, 14, 21, 28, 35 and 42. Motor-evoked potential tests were performed on the 42nd day. Histological analysis was performed after euthanasia. RESULTS: The group receiving EPO exhibited superior functional motor results on the BBB scale. IL-6 administration alone was not superior to the placebo treatment, and the IL-6 combination with EPO yielded worse results than did EPO alone. CONCLUSIONS: Using EPO after acute SCI in rats yielded benefits in functional recovery. The combination of EPO and IL-6 showed benefits, but with inferior results compared to those of isolated EPO; moreover, isolated use of IL-6 resulted in no benefit.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2019-08-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/clinics/article/view/16142910.6061/clinics/2019/e674Clinics; Vol. 74 (2019); e674Clinics; v. 74 (2019); e674Clinics; Vol. 74 (2019); e6741980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/161429/155373https://www.revistas.usp.br/clinics/article/view/161429/155374Copyright (c) 2019 Clinicsinfo:eu-repo/semantics/openAccessBarros, Alderico Girão Campos deCristante, Alexandre FogaçaSantos, Gustavo Bispo dosNatalino, Renato José MendonçaFerreira, Ricardo José RodriguezBarros-Filho, Tarcísio Eloy Pessoa de2019-08-26T11:48:12Zoai:revistas.usp.br:article/161429Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2019-08-26T11:48:12Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Evaluation of the effects of erythropoietin and interleukin-6 in rats submitted to acute spinal cord injury
title Evaluation of the effects of erythropoietin and interleukin-6 in rats submitted to acute spinal cord injury
spellingShingle Evaluation of the effects of erythropoietin and interleukin-6 in rats submitted to acute spinal cord injury
Barros, Alderico Girão Campos de
Interleukin-6
Erythropoietin
Spinal Cord Injuries
Central Nervous System/Injuries
Rats
title_short Evaluation of the effects of erythropoietin and interleukin-6 in rats submitted to acute spinal cord injury
title_full Evaluation of the effects of erythropoietin and interleukin-6 in rats submitted to acute spinal cord injury
title_fullStr Evaluation of the effects of erythropoietin and interleukin-6 in rats submitted to acute spinal cord injury
title_full_unstemmed Evaluation of the effects of erythropoietin and interleukin-6 in rats submitted to acute spinal cord injury
title_sort Evaluation of the effects of erythropoietin and interleukin-6 in rats submitted to acute spinal cord injury
author Barros, Alderico Girão Campos de
author_facet Barros, Alderico Girão Campos de
Cristante, Alexandre Fogaça
Santos, Gustavo Bispo dos
Natalino, Renato José Mendonça
Ferreira, Ricardo José Rodriguez
Barros-Filho, Tarcísio Eloy Pessoa de
author_role author
author2 Cristante, Alexandre Fogaça
Santos, Gustavo Bispo dos
Natalino, Renato José Mendonça
Ferreira, Ricardo José Rodriguez
Barros-Filho, Tarcísio Eloy Pessoa de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Barros, Alderico Girão Campos de
Cristante, Alexandre Fogaça
Santos, Gustavo Bispo dos
Natalino, Renato José Mendonça
Ferreira, Ricardo José Rodriguez
Barros-Filho, Tarcísio Eloy Pessoa de
dc.subject.por.fl_str_mv Interleukin-6
Erythropoietin
Spinal Cord Injuries
Central Nervous System/Injuries
Rats
topic Interleukin-6
Erythropoietin
Spinal Cord Injuries
Central Nervous System/Injuries
Rats
description OBJECTIVE: To evaluate the effects of interleukin-6 (IL-6) and erythropoietin (EPO) in experimental acute spinal cord injury (SCI) in rats. METHODS: Using standardized equipment, namely, a New York University (NYU) Impactor, a SCI was produced in 50 Wistar rats using a 10-g weight drop from a 12.5-mm height. The rats were divided into the following 5 groups of 10 animals each: ‘‘Group EPO’’, treated with erythropoietin only; ‘‘Group EPO + IL-6’’, treated with both substances; ‘‘Group IL-6’’, receiving IL-6 administration only; ‘‘Group Placebo’’, receiving a placebo solution; and ‘‘Group Sham’’, submitted to an incomplete procedure (only laminectomy, without SCI). All drugs and the placebo solution were administered intraperitoneally for three weeks. The animals were followed up for 42 days. Functional motor recovery was monitored by the Basso, Beattie, and Bresnahan (BBB) scale on days 2, 7, 14, 21, 28, 35 and 42. Motor-evoked potential tests were performed on the 42nd day. Histological analysis was performed after euthanasia. RESULTS: The group receiving EPO exhibited superior functional motor results on the BBB scale. IL-6 administration alone was not superior to the placebo treatment, and the IL-6 combination with EPO yielded worse results than did EPO alone. CONCLUSIONS: Using EPO after acute SCI in rats yielded benefits in functional recovery. The combination of EPO and IL-6 showed benefits, but with inferior results compared to those of isolated EPO; moreover, isolated use of IL-6 resulted in no benefit.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/161429
10.6061/clinics/2019/e674
url https://www.revistas.usp.br/clinics/article/view/161429
identifier_str_mv 10.6061/clinics/2019/e674
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/161429/155373
https://www.revistas.usp.br/clinics/article/view/161429/155374
dc.rights.driver.fl_str_mv Copyright (c) 2019 Clinics
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Clinics
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/xml
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 74 (2019); e674
Clinics; v. 74 (2019); e674
Clinics; Vol. 74 (2019); e674
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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