Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/214051 |
Resumo: | Objective: The aim of this study was to evaluate the best timing and feasibility of intrathecal application of sodium monosialoganglioside (GM1) after spinal cord contusion in Wistar rats as an experimental model. Methods: Forty Wistar rats were submitted to contusion spinal cord injury after laminectomy. The animals were randomized and divided into four groups: Group 1 – Intrathecal application of GM1 24 hours after contusion; Group 2 – Intrathecal application of GM1 48 hours after contusion; Group 3 – intrathecal application of GM1 72 hours after contusion; Group 4 – Sham, with laminectomy and intrathecal application of 0.5 mL of 0.9% saline solution, without contusion. The recovery of locomotor function was evaluated at seven different moments by the Basso, Beattie, and Bresnahan (BBB) test. They were also assessed by the horizontal ladder, with sensory-motor behavioral assessment criteria, pre-and postoperatively. Results: This experimental study showed better functional scores in the group submitted to the application of GM1, with statistically significant results, showing a mean increase when evaluated on known motor tests like the horizontal ladder and BBB, at all times of evaluation (p < 0.05), especially in group 2 (48 hours after spinal cord injury). Also, fewer mistakes and slips over the horizontal ladder were observed, and many points were achieved at the BBB scale analysis. Conclusion: The study demonstrated that the intrathecal application of GM1 after spinal cord contusion in Wistar rats is feasible. The application 48 hours after the injury presented the best functional results. |
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Clinics |
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Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in ratsSpinal cord injuriesG(M1) gangliosideInjectionsSpinalRatsWistarObjective: The aim of this study was to evaluate the best timing and feasibility of intrathecal application of sodium monosialoganglioside (GM1) after spinal cord contusion in Wistar rats as an experimental model. Methods: Forty Wistar rats were submitted to contusion spinal cord injury after laminectomy. The animals were randomized and divided into four groups: Group 1 – Intrathecal application of GM1 24 hours after contusion; Group 2 – Intrathecal application of GM1 48 hours after contusion; Group 3 – intrathecal application of GM1 72 hours after contusion; Group 4 – Sham, with laminectomy and intrathecal application of 0.5 mL of 0.9% saline solution, without contusion. The recovery of locomotor function was evaluated at seven different moments by the Basso, Beattie, and Bresnahan (BBB) test. They were also assessed by the horizontal ladder, with sensory-motor behavioral assessment criteria, pre-and postoperatively. Results: This experimental study showed better functional scores in the group submitted to the application of GM1, with statistically significant results, showing a mean increase when evaluated on known motor tests like the horizontal ladder and BBB, at all times of evaluation (p < 0.05), especially in group 2 (48 hours after spinal cord injury). Also, fewer mistakes and slips over the horizontal ladder were observed, and many points were achieved at the BBB scale analysis. Conclusion: The study demonstrated that the intrathecal application of GM1 after spinal cord contusion in Wistar rats is feasible. The application 48 hours after the injury presented the best functional results.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2023-07-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/21405110.1016/j.clinsp.2023.100228Clinics; Vol. 78 (2023); 100228Clinics; v. 78 (2023); 100228Clinics; Vol. 78 (2023); 1002281980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/214051/196282Copyright (c) 2023 Clinicsinfo:eu-repo/semantics/openAccessJorge, Daniel de Moraes FerreiraMarcon, Raphael MartusCristante, Alexandre FogaçaBarros Filho, Tarcísio Eloy PessoaSantos, Gustavo Bispo dos2023-07-06T13:05:40Zoai:revistas.usp.br:article/214051Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2023-07-06T13:05:40Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats |
title |
Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats |
spellingShingle |
Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats Jorge, Daniel de Moraes Ferreira Spinal cord injuries G(M1) ganglioside Injections Spinal Rats Wistar |
title_short |
Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats |
title_full |
Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats |
title_fullStr |
Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats |
title_full_unstemmed |
Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats |
title_sort |
Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats |
author |
Jorge, Daniel de Moraes Ferreira |
author_facet |
Jorge, Daniel de Moraes Ferreira Marcon, Raphael Martus Cristante, Alexandre Fogaça Barros Filho, Tarcísio Eloy Pessoa Santos, Gustavo Bispo dos |
author_role |
author |
author2 |
Marcon, Raphael Martus Cristante, Alexandre Fogaça Barros Filho, Tarcísio Eloy Pessoa Santos, Gustavo Bispo dos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Jorge, Daniel de Moraes Ferreira Marcon, Raphael Martus Cristante, Alexandre Fogaça Barros Filho, Tarcísio Eloy Pessoa Santos, Gustavo Bispo dos |
dc.subject.por.fl_str_mv |
Spinal cord injuries G(M1) ganglioside Injections Spinal Rats Wistar |
topic |
Spinal cord injuries G(M1) ganglioside Injections Spinal Rats Wistar |
description |
Objective: The aim of this study was to evaluate the best timing and feasibility of intrathecal application of sodium monosialoganglioside (GM1) after spinal cord contusion in Wistar rats as an experimental model. Methods: Forty Wistar rats were submitted to contusion spinal cord injury after laminectomy. The animals were randomized and divided into four groups: Group 1 – Intrathecal application of GM1 24 hours after contusion; Group 2 – Intrathecal application of GM1 48 hours after contusion; Group 3 – intrathecal application of GM1 72 hours after contusion; Group 4 – Sham, with laminectomy and intrathecal application of 0.5 mL of 0.9% saline solution, without contusion. The recovery of locomotor function was evaluated at seven different moments by the Basso, Beattie, and Bresnahan (BBB) test. They were also assessed by the horizontal ladder, with sensory-motor behavioral assessment criteria, pre-and postoperatively. Results: This experimental study showed better functional scores in the group submitted to the application of GM1, with statistically significant results, showing a mean increase when evaluated on known motor tests like the horizontal ladder and BBB, at all times of evaluation (p < 0.05), especially in group 2 (48 hours after spinal cord injury). Also, fewer mistakes and slips over the horizontal ladder were observed, and many points were achieved at the BBB scale analysis. Conclusion: The study demonstrated that the intrathecal application of GM1 after spinal cord contusion in Wistar rats is feasible. The application 48 hours after the injury presented the best functional results. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/214051 10.1016/j.clinsp.2023.100228 |
url |
https://www.revistas.usp.br/clinics/article/view/214051 |
identifier_str_mv |
10.1016/j.clinsp.2023.100228 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/214051/196282 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 78 (2023); 100228 Clinics; v. 78 (2023); 100228 Clinics; Vol. 78 (2023); 100228 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222767427092480 |