Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats

Detalhes bibliográficos
Autor(a) principal: Jorge, Daniel de Moraes Ferreira
Data de Publicação: 2023
Outros Autores: Marcon, Raphael Martus, Cristante, Alexandre Fogaça, Barros Filho, Tarcísio Eloy Pessoa, Santos, Gustavo Bispo dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/214051
Resumo: Objective: The aim of this study was to evaluate the best timing and feasibility of intrathecal application of sodium monosialoganglioside (GM1) after spinal cord contusion in Wistar rats as an experimental model. Methods: Forty Wistar rats were submitted to contusion spinal cord injury after laminectomy. The animals were randomized and divided into four groups: Group 1 – Intrathecal application of GM1 24 hours after contusion; Group 2 – Intrathecal application of GM1 48 hours after contusion; Group 3 – intrathecal application of GM1 72 hours after contusion; Group 4 – Sham, with laminectomy and intrathecal application of 0.5 mL of 0.9% saline solution, without contusion. The recovery of locomotor function was evaluated at seven different moments by the Basso, Beattie, and Bresnahan (BBB) test. They were also assessed by the horizontal ladder, with sensory-motor behavioral assessment criteria, pre-and postoperatively. Results: This experimental study showed better functional scores in the group submitted to the application of GM1, with statistically significant results, showing a mean increase when evaluated on known motor tests like the horizontal ladder and BBB, at all times of evaluation (p < 0.05), especially in group 2 (48 hours after spinal cord injury). Also, fewer mistakes and slips over the horizontal ladder were observed, and many points were achieved at the BBB scale analysis. Conclusion: The study demonstrated that the intrathecal application of GM1 after spinal cord contusion in Wistar rats is feasible. The application 48 hours after the injury presented the best functional results.
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spelling Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in ratsSpinal cord injuriesG(M1) gangliosideInjectionsSpinalRatsWistarObjective: The aim of this study was to evaluate the best timing and feasibility of intrathecal application of sodium monosialoganglioside (GM1) after spinal cord contusion in Wistar rats as an experimental model. Methods: Forty Wistar rats were submitted to contusion spinal cord injury after laminectomy. The animals were randomized and divided into four groups: Group 1 – Intrathecal application of GM1 24 hours after contusion; Group 2 – Intrathecal application of GM1 48 hours after contusion; Group 3 – intrathecal application of GM1 72 hours after contusion; Group 4 – Sham, with laminectomy and intrathecal application of 0.5 mL of 0.9% saline solution, without contusion. The recovery of locomotor function was evaluated at seven different moments by the Basso, Beattie, and Bresnahan (BBB) test. They were also assessed by the horizontal ladder, with sensory-motor behavioral assessment criteria, pre-and postoperatively. Results: This experimental study showed better functional scores in the group submitted to the application of GM1, with statistically significant results, showing a mean increase when evaluated on known motor tests like the horizontal ladder and BBB, at all times of evaluation (p < 0.05), especially in group 2 (48 hours after spinal cord injury). Also, fewer mistakes and slips over the horizontal ladder were observed, and many points were achieved at the BBB scale analysis. Conclusion: The study demonstrated that the intrathecal application of GM1 after spinal cord contusion in Wistar rats is feasible. The application 48 hours after the injury presented the best functional results.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2023-07-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/21405110.1016/j.clinsp.2023.100228Clinics; Vol. 78 (2023); 100228Clinics; v. 78 (2023); 100228Clinics; Vol. 78 (2023); 1002281980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/214051/196282Copyright (c) 2023 Clinicsinfo:eu-repo/semantics/openAccessJorge, Daniel de Moraes FerreiraMarcon, Raphael MartusCristante, Alexandre FogaçaBarros Filho, Tarcísio Eloy PessoaSantos, Gustavo Bispo dos2023-07-06T13:05:40Zoai:revistas.usp.br:article/214051Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2023-07-06T13:05:40Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats
title Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats
spellingShingle Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats
Jorge, Daniel de Moraes Ferreira
Spinal cord injuries
G(M1) ganglioside
Injections
Spinal
Rats
Wistar
title_short Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats
title_full Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats
title_fullStr Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats
title_full_unstemmed Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats
title_sort Evaluation of the effect of intrathecal GM1 in 24, 48, and 72 hours after acute spinal cord injury in rats
author Jorge, Daniel de Moraes Ferreira
author_facet Jorge, Daniel de Moraes Ferreira
Marcon, Raphael Martus
Cristante, Alexandre Fogaça
Barros Filho, Tarcísio Eloy Pessoa
Santos, Gustavo Bispo dos
author_role author
author2 Marcon, Raphael Martus
Cristante, Alexandre Fogaça
Barros Filho, Tarcísio Eloy Pessoa
Santos, Gustavo Bispo dos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Jorge, Daniel de Moraes Ferreira
Marcon, Raphael Martus
Cristante, Alexandre Fogaça
Barros Filho, Tarcísio Eloy Pessoa
Santos, Gustavo Bispo dos
dc.subject.por.fl_str_mv Spinal cord injuries
G(M1) ganglioside
Injections
Spinal
Rats
Wistar
topic Spinal cord injuries
G(M1) ganglioside
Injections
Spinal
Rats
Wistar
description Objective: The aim of this study was to evaluate the best timing and feasibility of intrathecal application of sodium monosialoganglioside (GM1) after spinal cord contusion in Wistar rats as an experimental model. Methods: Forty Wistar rats were submitted to contusion spinal cord injury after laminectomy. The animals were randomized and divided into four groups: Group 1 – Intrathecal application of GM1 24 hours after contusion; Group 2 – Intrathecal application of GM1 48 hours after contusion; Group 3 – intrathecal application of GM1 72 hours after contusion; Group 4 – Sham, with laminectomy and intrathecal application of 0.5 mL of 0.9% saline solution, without contusion. The recovery of locomotor function was evaluated at seven different moments by the Basso, Beattie, and Bresnahan (BBB) test. They were also assessed by the horizontal ladder, with sensory-motor behavioral assessment criteria, pre-and postoperatively. Results: This experimental study showed better functional scores in the group submitted to the application of GM1, with statistically significant results, showing a mean increase when evaluated on known motor tests like the horizontal ladder and BBB, at all times of evaluation (p < 0.05), especially in group 2 (48 hours after spinal cord injury). Also, fewer mistakes and slips over the horizontal ladder were observed, and many points were achieved at the BBB scale analysis. Conclusion: The study demonstrated that the intrathecal application of GM1 after spinal cord contusion in Wistar rats is feasible. The application 48 hours after the injury presented the best functional results.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-05
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/214051
10.1016/j.clinsp.2023.100228
url https://www.revistas.usp.br/clinics/article/view/214051
identifier_str_mv 10.1016/j.clinsp.2023.100228
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/214051/196282
dc.rights.driver.fl_str_mv Copyright (c) 2023 Clinics
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Clinics
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 78 (2023); 100228
Clinics; v. 78 (2023); 100228
Clinics; Vol. 78 (2023); 100228
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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