Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/79415 |
Resumo: | OBJECTIVE: Fibrin glues have not been consistently successful in preventing the dehiscence of high-risk colonic anastomoses. Fibrinogen and thrombin concentrations in glues determine their ability to function as sealants, healers, and/or adhesives. The objective of the current study was to compare the effects of different concentrations of fibrinogen and thrombin on bursting pressure, leaks, dehiscence, and morphology of high-risk ischemic colonic anastomoses using fibrin glue in rats. METHODS: Colonic anastomoses in adult female Sprague-Dawley rats (weight, 250-350 g) treated with fibrin glue containing different concentrations of fibrinogen and thrombin were evaluated at post-operative day 5. The interventions were low-risk (normal) or high-risk (ischemic) end-to-end colonic anastomoses using polypropylene sutures and topical application of fibrinogen at high (120 mg/mL) or low (40 mg/mL) concentrations and thrombin at high (1000 IU/mL) or low (500 IU/mL) concentrations. RESULTS: Ischemia alone, anastomosis alone, or both together reduced the bursting pressure. Glues containing a low fibrinogen concentration improved this parameter in all cases. High thrombin in combination with low fibrinogen also improved adherence exclusively in low-risk anastomoses. No differences were detected with respect to macroscopic parameters, histopathology, or hydroxyproline content at 5 days post-anastomosis. CONCLUSIONS: Fibrin glue with a low fibrinogen content normalizes the bursting pressure of high-risk ischemic left-colon anastomoses in rats at day 5 after surgery. |
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oai:revistas.usp.br:article/79415 |
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USP-19 |
network_name_str |
Clinics |
repository_id_str |
|
spelling |
Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses OBJECTIVE: Fibrin glues have not been consistently successful in preventing the dehiscence of high-risk colonic anastomoses. Fibrinogen and thrombin concentrations in glues determine their ability to function as sealants, healers, and/or adhesives. The objective of the current study was to compare the effects of different concentrations of fibrinogen and thrombin on bursting pressure, leaks, dehiscence, and morphology of high-risk ischemic colonic anastomoses using fibrin glue in rats. METHODS: Colonic anastomoses in adult female Sprague-Dawley rats (weight, 250-350 g) treated with fibrin glue containing different concentrations of fibrinogen and thrombin were evaluated at post-operative day 5. The interventions were low-risk (normal) or high-risk (ischemic) end-to-end colonic anastomoses using polypropylene sutures and topical application of fibrinogen at high (120 mg/mL) or low (40 mg/mL) concentrations and thrombin at high (1000 IU/mL) or low (500 IU/mL) concentrations. RESULTS: Ischemia alone, anastomosis alone, or both together reduced the bursting pressure. Glues containing a low fibrinogen concentration improved this parameter in all cases. High thrombin in combination with low fibrinogen also improved adherence exclusively in low-risk anastomoses. No differences were detected with respect to macroscopic parameters, histopathology, or hydroxyproline content at 5 days post-anastomosis. CONCLUSIONS: Fibrin glue with a low fibrinogen content normalizes the bursting pressure of high-risk ischemic left-colon anastomoses in rats at day 5 after surgery. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/7941510.6061/clinics/2014(04)07Clinics; Vol. 69 No. 4 (2014); 259-264Clinics; v. 69 n. 4 (2014); 259-264Clinics; Vol. 69 Núm. 4 (2014); 259-2641980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/79415/83470Buen, Eliseo Portilla-de Orozco-Mosqueda, Abel Leal-Cortés, Caridad Vázquez-Camacho, Gonzalo Fuentes-Orozco, Clotilde Alvarez-Villaseñor, Andrea Socorro Macías-Amezcua, Michel Dassaejv González-Ojeda, Alejandro info:eu-repo/semantics/openAccess2014-04-11T19:29:03Zoai:revistas.usp.br:article/79415Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2014-04-11T19:29:03Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses |
title |
Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses |
spellingShingle |
Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses Buen, Eliseo Portilla-de |
title_short |
Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses |
title_full |
Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses |
title_fullStr |
Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses |
title_full_unstemmed |
Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses |
title_sort |
Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses |
author |
Buen, Eliseo Portilla-de |
author_facet |
Buen, Eliseo Portilla-de Orozco-Mosqueda, Abel Leal-Cortés, Caridad Vázquez-Camacho, Gonzalo Fuentes-Orozco, Clotilde Alvarez-Villaseñor, Andrea Socorro Macías-Amezcua, Michel Dassaejv González-Ojeda, Alejandro |
author_role |
author |
author2 |
Orozco-Mosqueda, Abel Leal-Cortés, Caridad Vázquez-Camacho, Gonzalo Fuentes-Orozco, Clotilde Alvarez-Villaseñor, Andrea Socorro Macías-Amezcua, Michel Dassaejv González-Ojeda, Alejandro |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Buen, Eliseo Portilla-de Orozco-Mosqueda, Abel Leal-Cortés, Caridad Vázquez-Camacho, Gonzalo Fuentes-Orozco, Clotilde Alvarez-Villaseñor, Andrea Socorro Macías-Amezcua, Michel Dassaejv González-Ojeda, Alejandro |
description |
OBJECTIVE: Fibrin glues have not been consistently successful in preventing the dehiscence of high-risk colonic anastomoses. Fibrinogen and thrombin concentrations in glues determine their ability to function as sealants, healers, and/or adhesives. The objective of the current study was to compare the effects of different concentrations of fibrinogen and thrombin on bursting pressure, leaks, dehiscence, and morphology of high-risk ischemic colonic anastomoses using fibrin glue in rats. METHODS: Colonic anastomoses in adult female Sprague-Dawley rats (weight, 250-350 g) treated with fibrin glue containing different concentrations of fibrinogen and thrombin were evaluated at post-operative day 5. The interventions were low-risk (normal) or high-risk (ischemic) end-to-end colonic anastomoses using polypropylene sutures and topical application of fibrinogen at high (120 mg/mL) or low (40 mg/mL) concentrations and thrombin at high (1000 IU/mL) or low (500 IU/mL) concentrations. RESULTS: Ischemia alone, anastomosis alone, or both together reduced the bursting pressure. Glues containing a low fibrinogen concentration improved this parameter in all cases. High thrombin in combination with low fibrinogen also improved adherence exclusively in low-risk anastomoses. No differences were detected with respect to macroscopic parameters, histopathology, or hydroxyproline content at 5 days post-anastomosis. CONCLUSIONS: Fibrin glue with a low fibrinogen content normalizes the bursting pressure of high-risk ischemic left-colon anastomoses in rats at day 5 after surgery. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/79415 10.6061/clinics/2014(04)07 |
url |
https://www.revistas.usp.br/clinics/article/view/79415 |
identifier_str_mv |
10.6061/clinics/2014(04)07 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/79415/83470 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 69 No. 4 (2014); 259-264 Clinics; v. 69 n. 4 (2014); 259-264 Clinics; Vol. 69 Núm. 4 (2014); 259-264 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222761169190912 |