Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation

Detalhes bibliográficos
Autor(a) principal: Hamid, Adila A
Data de Publicação: 2012
Outros Autores: Idrus, Ruszymah Bt Hj, Saim, Aminuddin Bin, Sathappan, Somasumdaram, Chua, Kien-Hui
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/19674
Resumo: OBJECTIVES: Understanding the changes in chondrogenic gene expression that are involved in the differentiation of human adipose-derived stem cells to chondrogenic cells is important prior to using this approach for cartilage repair. The aims of the study were to characterize human adipose-derived stem cells and to examine chondrogenic gene expression after one, two, and three weeks of induction. MATERIALS AND METHODS: Human adipose-derived stem cells at passage 4 were evaluated by flow cytometry to examine the expression of surface markers. These adipose-derived stem cells were tested for adipogenic and osteogenic differentiation capacity. Ribonucleic acid was extracted from the cells for quantitative polymerase chain reaction analysis to determine the expression levels of chondrogenic genes after chondrogenic induction. RESULTS: Human adipose-derived stem cells were strongly positive for the mesenchymal markers CD90, CD73, CD44, CD9, and histocompatibility antigen and successfully differentiated into adipogenic and osteogenic lineages. The human adipose-derived stem cells aggregated and formed a dense matrix after chondrogenic induction. The expression of chondrogenic genes (collagen type II, aggrecan core protein, collagen type XI, COMP, and ELASTIN) was significantly higher after the first week of induction. However, a significantly elevated expression of collagen type X was observed after three weeks of chondrogenic induction. CONCLUSION: Human adipose-derived stem cells retain stem cell characteristics after expansion in culture to passage 4 and serve as a feasible source of cells for cartilage regeneration. Chondrogenesis in human adiposederived stem cells was most prominent after one week of chondrogenic induction.
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spelling Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiationChondrogenesisCell-based therapyCartilageChondrocytesAdiposeStem cellsDifferentiationOBJECTIVES: Understanding the changes in chondrogenic gene expression that are involved in the differentiation of human adipose-derived stem cells to chondrogenic cells is important prior to using this approach for cartilage repair. The aims of the study were to characterize human adipose-derived stem cells and to examine chondrogenic gene expression after one, two, and three weeks of induction. MATERIALS AND METHODS: Human adipose-derived stem cells at passage 4 were evaluated by flow cytometry to examine the expression of surface markers. These adipose-derived stem cells were tested for adipogenic and osteogenic differentiation capacity. Ribonucleic acid was extracted from the cells for quantitative polymerase chain reaction analysis to determine the expression levels of chondrogenic genes after chondrogenic induction. RESULTS: Human adipose-derived stem cells were strongly positive for the mesenchymal markers CD90, CD73, CD44, CD9, and histocompatibility antigen and successfully differentiated into adipogenic and osteogenic lineages. The human adipose-derived stem cells aggregated and formed a dense matrix after chondrogenic induction. The expression of chondrogenic genes (collagen type II, aggrecan core protein, collagen type XI, COMP, and ELASTIN) was significantly higher after the first week of induction. However, a significantly elevated expression of collagen type X was observed after three weeks of chondrogenic induction. CONCLUSION: Human adipose-derived stem cells retain stem cell characteristics after expansion in culture to passage 4 and serve as a feasible source of cells for cartilage regeneration. Chondrogenesis in human adiposederived stem cells was most prominent after one week of chondrogenic induction.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1967410.6061/clinics/2012(02)03Clinics; Vol. 67 No. 2 (2012); 099-106Clinics; v. 67 n. 2 (2012); 099-106Clinics; Vol. 67 Núm. 2 (2012); 099-1061980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/19674/21738Hamid, Adila AIdrus, Ruszymah Bt HjSaim, Aminuddin BinSathappan, SomasumdaramChua, Kien-Huiinfo:eu-repo/semantics/openAccess2012-05-24T18:50:19Zoai:revistas.usp.br:article/19674Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-24T18:50:19Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation
title Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation
spellingShingle Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation
Hamid, Adila A
Chondrogenesis
Cell-based therapy
Cartilage
Chondrocytes
Adipose
Stem cells
Differentiation
title_short Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation
title_full Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation
title_fullStr Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation
title_full_unstemmed Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation
title_sort Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation
author Hamid, Adila A
author_facet Hamid, Adila A
Idrus, Ruszymah Bt Hj
Saim, Aminuddin Bin
Sathappan, Somasumdaram
Chua, Kien-Hui
author_role author
author2 Idrus, Ruszymah Bt Hj
Saim, Aminuddin Bin
Sathappan, Somasumdaram
Chua, Kien-Hui
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Hamid, Adila A
Idrus, Ruszymah Bt Hj
Saim, Aminuddin Bin
Sathappan, Somasumdaram
Chua, Kien-Hui
dc.subject.por.fl_str_mv Chondrogenesis
Cell-based therapy
Cartilage
Chondrocytes
Adipose
Stem cells
Differentiation
topic Chondrogenesis
Cell-based therapy
Cartilage
Chondrocytes
Adipose
Stem cells
Differentiation
description OBJECTIVES: Understanding the changes in chondrogenic gene expression that are involved in the differentiation of human adipose-derived stem cells to chondrogenic cells is important prior to using this approach for cartilage repair. The aims of the study were to characterize human adipose-derived stem cells and to examine chondrogenic gene expression after one, two, and three weeks of induction. MATERIALS AND METHODS: Human adipose-derived stem cells at passage 4 were evaluated by flow cytometry to examine the expression of surface markers. These adipose-derived stem cells were tested for adipogenic and osteogenic differentiation capacity. Ribonucleic acid was extracted from the cells for quantitative polymerase chain reaction analysis to determine the expression levels of chondrogenic genes after chondrogenic induction. RESULTS: Human adipose-derived stem cells were strongly positive for the mesenchymal markers CD90, CD73, CD44, CD9, and histocompatibility antigen and successfully differentiated into adipogenic and osteogenic lineages. The human adipose-derived stem cells aggregated and formed a dense matrix after chondrogenic induction. The expression of chondrogenic genes (collagen type II, aggrecan core protein, collagen type XI, COMP, and ELASTIN) was significantly higher after the first week of induction. However, a significantly elevated expression of collagen type X was observed after three weeks of chondrogenic induction. CONCLUSION: Human adipose-derived stem cells retain stem cell characteristics after expansion in culture to passage 4 and serve as a feasible source of cells for cartilage regeneration. Chondrogenesis in human adiposederived stem cells was most prominent after one week of chondrogenic induction.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/19674
10.6061/clinics/2012(02)03
url https://www.revistas.usp.br/clinics/article/view/19674
identifier_str_mv 10.6061/clinics/2012(02)03
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/19674/21738
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 67 No. 2 (2012); 099-106
Clinics; v. 67 n. 2 (2012); 099-106
Clinics; Vol. 67 Núm. 2 (2012); 099-106
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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